MaHAK5, a Potassium Transporter of Banana, Enhanced Potassium Uptake in Transgenic <i>Arabidopsis</i> under Low Potassium Conditions
Potassium (K<sup>+</sup>) is one of the most important macronutrients for plant growth and development. It is generally accepted that the KUP/HAK/KT transporters play essential roles in K<sup>+</sup> uptake at low concentrations. However, their physiological functions in bana...
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MDPI AG
2022-12-01
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| Series: | Horticulturae |
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| Online Access: | https://www.mdpi.com/2311-7524/9/1/10 |
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| author | Bangting Wu Yanling Xie Dandan Xiang Ganjun Yi Hong Liu Chunyu Li Siwen Liu |
| author_facet | Bangting Wu Yanling Xie Dandan Xiang Ganjun Yi Hong Liu Chunyu Li Siwen Liu |
| author_sort | Bangting Wu |
| collection | DOAJ |
| description | Potassium (K<sup>+</sup>) is one of the most important macronutrients for plant growth and development. It is generally accepted that the KUP/HAK/KT transporters play essential roles in K<sup>+</sup> uptake at low concentrations. However, their physiological functions in bananas remain unknown. Here, we cloned MaHAK5 and analyzed its functions in banana (<i>Musa acuminata</i>). Gene expression analysis showed that MaHAK5 was upregulated in the roots and leaves in the early stage of low K<sup>+</sup> (LK) stress. MaHAK5 was localized in the cytomembrane. The expression of MaHAK5 improved the growth of the low K<sup>+</sup>-sensitive yeast mutant R5421 at different K<sup>+</sup> supply levels. Overexpression of <i>MaHAK5</i> in <i>Arabidopsis thaliana</i> significantly enhanced the ability for K<sup>+</sup> uptake and increased the chlorophyll content under LK stress. These results indicate that <i>MaHAK5</i> plays a crucial role in maintaining K<sup>+</sup> uptake in bananas. |
| first_indexed | 2024-03-09T12:30:59Z |
| format | Article |
| id | doaj.art-b284855147564f329ce6087d6d783d8f |
| institution | Directory Open Access Journal |
| issn | 2311-7524 |
| language | English |
| last_indexed | 2024-03-09T12:30:59Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Horticulturae |
| spelling | doaj.art-b284855147564f329ce6087d6d783d8f2023-11-30T22:29:34ZengMDPI AGHorticulturae2311-75242022-12-01911010.3390/horticulturae9010010MaHAK5, a Potassium Transporter of Banana, Enhanced Potassium Uptake in Transgenic <i>Arabidopsis</i> under Low Potassium ConditionsBangting Wu0Yanling Xie1Dandan Xiang2Ganjun Yi3Hong Liu4Chunyu Li5Siwen Liu6Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou 510640, ChinaKey Laboratory of South Subtropical Fruit Biology and Genetic Resource Utilization, Ministry of Agriculture, Key Laboratory of Tropical and Subtropical Fruit Tree Research of Guangdong Province, Institution of Fruit Tree Research, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, ChinaKey Laboratory of South Subtropical Fruit Biology and Genetic Resource Utilization, Ministry of Agriculture, Key Laboratory of Tropical and Subtropical Fruit Tree Research of Guangdong Province, Institution of Fruit Tree Research, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, ChinaGuangdong Laboratory for Lingnan Modern Agriculture, Guangzhou 510640, ChinaState Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, College of Resources and Environment, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaGuangdong Laboratory for Lingnan Modern Agriculture, Guangzhou 510640, ChinaGuangdong Laboratory for Lingnan Modern Agriculture, Guangzhou 510640, ChinaPotassium (K<sup>+</sup>) is one of the most important macronutrients for plant growth and development. It is generally accepted that the KUP/HAK/KT transporters play essential roles in K<sup>+</sup> uptake at low concentrations. However, their physiological functions in bananas remain unknown. Here, we cloned MaHAK5 and analyzed its functions in banana (<i>Musa acuminata</i>). Gene expression analysis showed that MaHAK5 was upregulated in the roots and leaves in the early stage of low K<sup>+</sup> (LK) stress. MaHAK5 was localized in the cytomembrane. The expression of MaHAK5 improved the growth of the low K<sup>+</sup>-sensitive yeast mutant R5421 at different K<sup>+</sup> supply levels. Overexpression of <i>MaHAK5</i> in <i>Arabidopsis thaliana</i> significantly enhanced the ability for K<sup>+</sup> uptake and increased the chlorophyll content under LK stress. These results indicate that <i>MaHAK5</i> plays a crucial role in maintaining K<sup>+</sup> uptake in bananas.https://www.mdpi.com/2311-7524/9/1/10bananaMaHAK5Potassium transporterK<sup>+</sup> uptakelow K<sup>+</sup> stress |
| spellingShingle | Bangting Wu Yanling Xie Dandan Xiang Ganjun Yi Hong Liu Chunyu Li Siwen Liu MaHAK5, a Potassium Transporter of Banana, Enhanced Potassium Uptake in Transgenic <i>Arabidopsis</i> under Low Potassium Conditions Horticulturae banana MaHAK5 Potassium transporter K<sup>+</sup> uptake low K<sup>+</sup> stress |
| title | MaHAK5, a Potassium Transporter of Banana, Enhanced Potassium Uptake in Transgenic <i>Arabidopsis</i> under Low Potassium Conditions |
| title_full | MaHAK5, a Potassium Transporter of Banana, Enhanced Potassium Uptake in Transgenic <i>Arabidopsis</i> under Low Potassium Conditions |
| title_fullStr | MaHAK5, a Potassium Transporter of Banana, Enhanced Potassium Uptake in Transgenic <i>Arabidopsis</i> under Low Potassium Conditions |
| title_full_unstemmed | MaHAK5, a Potassium Transporter of Banana, Enhanced Potassium Uptake in Transgenic <i>Arabidopsis</i> under Low Potassium Conditions |
| title_short | MaHAK5, a Potassium Transporter of Banana, Enhanced Potassium Uptake in Transgenic <i>Arabidopsis</i> under Low Potassium Conditions |
| title_sort | mahak5 a potassium transporter of banana enhanced potassium uptake in transgenic i arabidopsis i under low potassium conditions |
| topic | banana MaHAK5 Potassium transporter K<sup>+</sup> uptake low K<sup>+</sup> stress |
| url | https://www.mdpi.com/2311-7524/9/1/10 |
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