Highlighting the Crude Oil Bioremediation Potential of Marine Fungi Isolated from the Port of Oran (Algeria)
While over hundreds of terrestrial fungal genera have been shown to play important roles in the biodegradation of hydrocarbons, few studies have so far focused on the fungal bioremediation potential of petroleum in the marine environment. In this study, the culturable fungal communities occurring in...
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MDPI AG
2020-05-01
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| Online Access: | https://www.mdpi.com/1424-2818/12/5/196 |
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| author | Ahlem Maamar Marie-Elisabeth Lucchesi Stella Debaets Nicolas Nguyen van Long Maxence Quemener Emmanuel Coton Mohammed Bouderbala Gaëtan Burgaud Amaria Matallah-Boutiba |
| author_facet | Ahlem Maamar Marie-Elisabeth Lucchesi Stella Debaets Nicolas Nguyen van Long Maxence Quemener Emmanuel Coton Mohammed Bouderbala Gaëtan Burgaud Amaria Matallah-Boutiba |
| author_sort | Ahlem Maamar |
| collection | DOAJ |
| description | While over hundreds of terrestrial fungal genera have been shown to play important roles in the biodegradation of hydrocarbons, few studies have so far focused on the fungal bioremediation potential of petroleum in the marine environment. In this study, the culturable fungal communities occurring in the port of Oran in Algeria, considered here as a chronically-contaminated site, have been mainly analyzed in terms of species richness. A collection of 84 filamentous fungi has been established from seawater samples and then the fungi were screened for their ability to utilize and degrade crude oil. A total of 12 isolates were able to utilize crude oil as a unique carbon source, from which 4 were defined as the most promising biodegrading isolates based on a screening test using 2,6-dichlorophenol indophenol as a proxy to highlight their ability to metabolize crude oil. The biosurfactant production capability was also tested and, interestingly, the oil spreading and drop-collapse tests highlighted that the 4 most promising isolates were also those able to produce the highest quantity of biosurfactants. The results generated in this study demonstrate that the most promising fungal isolates, namely <i>Penicillium polonicum</i> AMF16, <i>P. chrysogenum</i> AMF47 and 2 isolates (AMF40 and AMF74) affiliated to <i>P. cyclopium</i>, appear to be interesting candidates for bioremediation of crude oil pollution in the marine environment within the frame of bioaugmentation or biostimulation processes. |
| first_indexed | 2024-03-10T19:48:52Z |
| format | Article |
| id | doaj.art-b287fa6bdce24b868b6e812e4425fc05 |
| institution | Directory Open Access Journal |
| issn | 1424-2818 |
| language | English |
| last_indexed | 2024-03-10T19:48:52Z |
| publishDate | 2020-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Diversity |
| spelling | doaj.art-b287fa6bdce24b868b6e812e4425fc052023-11-20T00:34:29ZengMDPI AGDiversity1424-28182020-05-0112519610.3390/d12050196Highlighting the Crude Oil Bioremediation Potential of Marine Fungi Isolated from the Port of Oran (Algeria)Ahlem Maamar0Marie-Elisabeth Lucchesi1Stella Debaets2Nicolas Nguyen van Long3Maxence Quemener4Emmanuel Coton5Mohammed Bouderbala6Gaëtan Burgaud7Amaria Matallah-Boutiba8Laboratoire Réseau de Surveillance Environnementale (LRSE), Department of Biology, Faculty of Life and Nature, University of Oran, Oran 31000, AlgeriaLaboratoire Universitaire de Biodiversité et Ecologie Microbienne, Université de Brest, F-29280 Plouzané, FranceLaboratoire Universitaire de Biodiversité et Ecologie Microbienne, Université de Brest, F-29280 Plouzané, FranceUMT19.03.01 ALTER’iX, Food Quality & Safety Unit, ADRIA Food Technology Institute, 29000 Quimper, FranceLaboratoire Universitaire de Biodiversité et Ecologie Microbienne, Université de Brest, F-29280 Plouzané, FranceLaboratoire Universitaire de Biodiversité et Ecologie Microbienne, Université de Brest, F-29280 Plouzané, FranceLaboratoire Réseau de Surveillance Environnementale (LRSE), Department of Biology, Faculty of Life and Nature, University of Oran, Oran 31000, AlgeriaLaboratoire Universitaire de Biodiversité et Ecologie Microbienne, Université de Brest, F-29280 Plouzané, FranceLaboratoire Réseau de Surveillance Environnementale (LRSE), Department of Biology, Faculty of Life and Nature, University of Oran, Oran 31000, AlgeriaWhile over hundreds of terrestrial fungal genera have been shown to play important roles in the biodegradation of hydrocarbons, few studies have so far focused on the fungal bioremediation potential of petroleum in the marine environment. In this study, the culturable fungal communities occurring in the port of Oran in Algeria, considered here as a chronically-contaminated site, have been mainly analyzed in terms of species richness. A collection of 84 filamentous fungi has been established from seawater samples and then the fungi were screened for their ability to utilize and degrade crude oil. A total of 12 isolates were able to utilize crude oil as a unique carbon source, from which 4 were defined as the most promising biodegrading isolates based on a screening test using 2,6-dichlorophenol indophenol as a proxy to highlight their ability to metabolize crude oil. The biosurfactant production capability was also tested and, interestingly, the oil spreading and drop-collapse tests highlighted that the 4 most promising isolates were also those able to produce the highest quantity of biosurfactants. The results generated in this study demonstrate that the most promising fungal isolates, namely <i>Penicillium polonicum</i> AMF16, <i>P. chrysogenum</i> AMF47 and 2 isolates (AMF40 and AMF74) affiliated to <i>P. cyclopium</i>, appear to be interesting candidates for bioremediation of crude oil pollution in the marine environment within the frame of bioaugmentation or biostimulation processes.https://www.mdpi.com/1424-2818/12/5/196marine fungicrude oilbiodegradationDCPIPbiosurfactant |
| spellingShingle | Ahlem Maamar Marie-Elisabeth Lucchesi Stella Debaets Nicolas Nguyen van Long Maxence Quemener Emmanuel Coton Mohammed Bouderbala Gaëtan Burgaud Amaria Matallah-Boutiba Highlighting the Crude Oil Bioremediation Potential of Marine Fungi Isolated from the Port of Oran (Algeria) Diversity marine fungi crude oil biodegradation DCPIP biosurfactant |
| title | Highlighting the Crude Oil Bioremediation Potential of Marine Fungi Isolated from the Port of Oran (Algeria) |
| title_full | Highlighting the Crude Oil Bioremediation Potential of Marine Fungi Isolated from the Port of Oran (Algeria) |
| title_fullStr | Highlighting the Crude Oil Bioremediation Potential of Marine Fungi Isolated from the Port of Oran (Algeria) |
| title_full_unstemmed | Highlighting the Crude Oil Bioremediation Potential of Marine Fungi Isolated from the Port of Oran (Algeria) |
| title_short | Highlighting the Crude Oil Bioremediation Potential of Marine Fungi Isolated from the Port of Oran (Algeria) |
| title_sort | highlighting the crude oil bioremediation potential of marine fungi isolated from the port of oran algeria |
| topic | marine fungi crude oil biodegradation DCPIP biosurfactant |
| url | https://www.mdpi.com/1424-2818/12/5/196 |
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