(INVITED)Nanocoated fiber label-free biosensing for perfluorooctanoic acid detection by lossy mode resonance
The determination of per- and polyfluoroalkyl substances (PFAS) in environmental samples, such as drinking waters, requires the design of high performing and versatile sensing strategies. Label-free biosensing platforms based on specialty fiber optics are a valid option to face this challenge. Among...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2021-12-01
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| Series: | Results in Optics |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666950121000717 |
| _version_ | 1819319216577183744 |
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| author | Giulia Moro Francesco Chiavaioli Stefano Liberi Pablo Zubiate Ignacio Del Villar Alessandro Angelini Karolien De Wael Francesco Baldini Ligia Maria Moretto Ambra Giannetti |
| author_facet | Giulia Moro Francesco Chiavaioli Stefano Liberi Pablo Zubiate Ignacio Del Villar Alessandro Angelini Karolien De Wael Francesco Baldini Ligia Maria Moretto Ambra Giannetti |
| author_sort | Giulia Moro |
| collection | DOAJ |
| description | The determination of per- and polyfluoroalkyl substances (PFAS) in environmental samples, such as drinking waters, requires the design of high performing and versatile sensing strategies. Label-free biosensing platforms based on specialty fiber optics are a valid option to face this challenge. Among them, lossy mode resonance (LMR) fiber optic biosensors are showing remarkable performance in terms of detection limit, selectivity, and reproducibility. The detection of small molecules, such as perfluorooctanoic acid (PFOA), can be achieved with the help of well-designed biological recognition layers. In this study, the biosensing potentialities of a label-free LMR-assisted optical platform based on nanocoated fibers are investigated. Delipidated human serum albumin (hSA) was used as biological recognition layer for PFOA in aqueous solution. Different fiber functionalization protocols based on the covalent immobilization of hSA were tested. The conformational changes related to the formation of hSA/PFOA complex were followed via optical monitoring of LMR spectral shift, showing a trend that can be modeled with Langmuir adsorption isotherm. These results confirmed the potentiality of LMR-based fiber biosensors for the detection of small molecules, such as PFOA, in synthetic samples. |
| first_indexed | 2024-12-24T11:00:10Z |
| format | Article |
| id | doaj.art-b288b41736424b84998466bdb4482105 |
| institution | Directory Open Access Journal |
| issn | 2666-9501 |
| language | English |
| last_indexed | 2024-12-24T11:00:10Z |
| publishDate | 2021-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Results in Optics |
| spelling | doaj.art-b288b41736424b84998466bdb44821052022-12-21T16:58:44ZengElsevierResults in Optics2666-95012021-12-015100123(INVITED)Nanocoated fiber label-free biosensing for perfluorooctanoic acid detection by lossy mode resonanceGiulia Moro0Francesco Chiavaioli1Stefano Liberi2Pablo Zubiate3Ignacio Del Villar4Alessandro Angelini5Karolien De Wael6Francesco Baldini7Ligia Maria Moretto8Ambra Giannetti9Department of Molecular Sciences and Nanosystems, Ca’ Foscari University of Venice, Via Torino 155, 30172 Mestre, Italy; AXES Research Group, University of Antwerp, Groenenborgerlaan 171, 2020, Antwerp, Belgium; NanoLab Center of Excellence, University of Antwerp, Groenenborgerlaan 171, 2020 Antwerp, BelgiumInstitute of Applied Physics “Nello Carrara”, National Research Council of Italy (CNR), 50019 Sesto Fiorentino, Firenze, Italy; Corresponding author.Department of Biology, University of Padua, Via U. Bassi 58, 35131 Padova, ItalyElectrical and Electronic Engineering Department, Public University of Navarra, 31006 Pamplona, SpainElectrical and Electronic Engineering Department, Public University of Navarra, 31006 Pamplona, Spain; Institute of Smart Cities (ISC), Public University of Navarra, 31006 Pamplona, SpainDepartment of Molecular Sciences and Nanosystems, Ca’ Foscari University of Venice, Via Torino 155, 30172 Mestre, Italy; European Centre for Living Technology (ECLT), Ca’ Bottacin, Dorsoduro 3911, Calle Crosera, 30123 Venice, ItalyAXES Research Group, University of Antwerp, Groenenborgerlaan 171, 2020, Antwerp, Belgium; NanoLab Center of Excellence, University of Antwerp, Groenenborgerlaan 171, 2020 Antwerp, BelgiumInstitute of Applied Physics “Nello Carrara”, National Research Council of Italy (CNR), 50019 Sesto Fiorentino, Firenze, ItalyDepartment of Molecular Sciences and Nanosystems, Ca’ Foscari University of Venice, Via Torino 155, 30172 Mestre, ItalyInstitute of Applied Physics “Nello Carrara”, National Research Council of Italy (CNR), 50019 Sesto Fiorentino, Firenze, ItalyThe determination of per- and polyfluoroalkyl substances (PFAS) in environmental samples, such as drinking waters, requires the design of high performing and versatile sensing strategies. Label-free biosensing platforms based on specialty fiber optics are a valid option to face this challenge. Among them, lossy mode resonance (LMR) fiber optic biosensors are showing remarkable performance in terms of detection limit, selectivity, and reproducibility. The detection of small molecules, such as perfluorooctanoic acid (PFOA), can be achieved with the help of well-designed biological recognition layers. In this study, the biosensing potentialities of a label-free LMR-assisted optical platform based on nanocoated fibers are investigated. Delipidated human serum albumin (hSA) was used as biological recognition layer for PFOA in aqueous solution. Different fiber functionalization protocols based on the covalent immobilization of hSA were tested. The conformational changes related to the formation of hSA/PFOA complex were followed via optical monitoring of LMR spectral shift, showing a trend that can be modeled with Langmuir adsorption isotherm. These results confirmed the potentiality of LMR-based fiber biosensors for the detection of small molecules, such as PFOA, in synthetic samples.http://www.sciencedirect.com/science/article/pii/S2666950121000717Nanocoated fiber sensorLossy mode resonanceSmall target detectionDelipidated human serum albuminPerfluorooctanoic acidLabel-free biosensing |
| spellingShingle | Giulia Moro Francesco Chiavaioli Stefano Liberi Pablo Zubiate Ignacio Del Villar Alessandro Angelini Karolien De Wael Francesco Baldini Ligia Maria Moretto Ambra Giannetti (INVITED)Nanocoated fiber label-free biosensing for perfluorooctanoic acid detection by lossy mode resonance Results in Optics Nanocoated fiber sensor Lossy mode resonance Small target detection Delipidated human serum albumin Perfluorooctanoic acid Label-free biosensing |
| title | (INVITED)Nanocoated fiber label-free biosensing for perfluorooctanoic acid detection by lossy mode resonance |
| title_full | (INVITED)Nanocoated fiber label-free biosensing for perfluorooctanoic acid detection by lossy mode resonance |
| title_fullStr | (INVITED)Nanocoated fiber label-free biosensing for perfluorooctanoic acid detection by lossy mode resonance |
| title_full_unstemmed | (INVITED)Nanocoated fiber label-free biosensing for perfluorooctanoic acid detection by lossy mode resonance |
| title_short | (INVITED)Nanocoated fiber label-free biosensing for perfluorooctanoic acid detection by lossy mode resonance |
| title_sort | invited nanocoated fiber label free biosensing for perfluorooctanoic acid detection by lossy mode resonance |
| topic | Nanocoated fiber sensor Lossy mode resonance Small target detection Delipidated human serum albumin Perfluorooctanoic acid Label-free biosensing |
| url | http://www.sciencedirect.com/science/article/pii/S2666950121000717 |
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