Silencing a Candidate Nematode Effector Gene Corresponding to the Tomato Resistance Gene Mi-1 Leads to Acquisition of Virulence
The Mi-1 gene in tomato confers effective resistance against several species of root-knot nematode, including Meloidogyne javanica. A strain of M. javanica that can reproduce on tomato with Mi-1 was obtained from a culture of an avirulent strain after greenhouse selection. DNA blots and amplified fr...
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| Format: | Article |
| Language: | English |
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The American Phytopathological Society
2008-05-01
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| Series: | Molecular Plant-Microbe Interactions |
| Subjects: | |
| Online Access: | https://apsjournals.apsnet.org/doi/10.1094/MPMI-21-5-0576 |
| _version_ | 1818647036257370112 |
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| author | Cynthia A. Gleason Qingli L. Liu Valerie M. Williamson |
| author_facet | Cynthia A. Gleason Qingli L. Liu Valerie M. Williamson |
| author_sort | Cynthia A. Gleason |
| collection | DOAJ |
| description | The Mi-1 gene in tomato confers effective resistance against several species of root-knot nematode, including Meloidogyne javanica. A strain of M. javanica that can reproduce on tomato with Mi-1 was obtained from a culture of an avirulent strain after greenhouse selection. DNA blots and amplified fragment length polymorphism (AFLP) analysis indicated that the two nematode strains are closely related. Expression patterns visualized as cDNA AFLPs were nearly identical except for a cDNA fragment, Cg-1, that was present in the avirulent strain but not in the virulent strain. DNA blots showed that Cg-1 corresponds to a member of a small gene family with one or more copies missing in the virulent strain compared with the avirulent strain. Except for the presence of a histone stem loop near the 3′ end of the transcript, Cg-1 shows no similarity to other sequences in GenBank. The longest open reading frame is 32 amino acids and initiates at the fourth AUG in the predicted transcript. When nematode juveniles of the Mi-1-avirulent strain were soaked in dsRNA corresponding to part of the predicted Cg-1 transcript, they produced progeny that were virulent on tomato carrying the Mi-1 gene, strongly suggesting that Cg-1 is required in the nematode for Mi-1-mediated resistance. |
| first_indexed | 2024-12-17T00:56:08Z |
| format | Article |
| id | doaj.art-b28b30ef36ae4b5382880b0abf2c86ba |
| institution | Directory Open Access Journal |
| issn | 0894-0282 1943-7706 |
| language | English |
| last_indexed | 2024-12-17T00:56:08Z |
| publishDate | 2008-05-01 |
| publisher | The American Phytopathological Society |
| record_format | Article |
| series | Molecular Plant-Microbe Interactions |
| spelling | doaj.art-b28b30ef36ae4b5382880b0abf2c86ba2022-12-21T22:09:36ZengThe American Phytopathological SocietyMolecular Plant-Microbe Interactions0894-02821943-77062008-05-0121557658510.1094/MPMI-21-5-0576Silencing a Candidate Nematode Effector Gene Corresponding to the Tomato Resistance Gene Mi-1 Leads to Acquisition of VirulenceCynthia A. GleasonQingli L. LiuValerie M. WilliamsonThe Mi-1 gene in tomato confers effective resistance against several species of root-knot nematode, including Meloidogyne javanica. A strain of M. javanica that can reproduce on tomato with Mi-1 was obtained from a culture of an avirulent strain after greenhouse selection. DNA blots and amplified fragment length polymorphism (AFLP) analysis indicated that the two nematode strains are closely related. Expression patterns visualized as cDNA AFLPs were nearly identical except for a cDNA fragment, Cg-1, that was present in the avirulent strain but not in the virulent strain. DNA blots showed that Cg-1 corresponds to a member of a small gene family with one or more copies missing in the virulent strain compared with the avirulent strain. Except for the presence of a histone stem loop near the 3′ end of the transcript, Cg-1 shows no similarity to other sequences in GenBank. The longest open reading frame is 32 amino acids and initiates at the fourth AUG in the predicted transcript. When nematode juveniles of the Mi-1-avirulent strain were soaked in dsRNA corresponding to part of the predicted Cg-1 transcript, they produced progeny that were virulent on tomato carrying the Mi-1 gene, strongly suggesting that Cg-1 is required in the nematode for Mi-1-mediated resistance.https://apsjournals.apsnet.org/doi/10.1094/MPMI-21-5-0576avirulencehistone hairpinR genesORF-mRNA |
| spellingShingle | Cynthia A. Gleason Qingli L. Liu Valerie M. Williamson Silencing a Candidate Nematode Effector Gene Corresponding to the Tomato Resistance Gene Mi-1 Leads to Acquisition of Virulence Molecular Plant-Microbe Interactions avirulence histone hairpin R gene sORF-mRNA |
| title | Silencing a Candidate Nematode Effector Gene Corresponding to the Tomato Resistance Gene Mi-1 Leads to Acquisition of Virulence |
| title_full | Silencing a Candidate Nematode Effector Gene Corresponding to the Tomato Resistance Gene Mi-1 Leads to Acquisition of Virulence |
| title_fullStr | Silencing a Candidate Nematode Effector Gene Corresponding to the Tomato Resistance Gene Mi-1 Leads to Acquisition of Virulence |
| title_full_unstemmed | Silencing a Candidate Nematode Effector Gene Corresponding to the Tomato Resistance Gene Mi-1 Leads to Acquisition of Virulence |
| title_short | Silencing a Candidate Nematode Effector Gene Corresponding to the Tomato Resistance Gene Mi-1 Leads to Acquisition of Virulence |
| title_sort | silencing a candidate nematode effector gene corresponding to the tomato resistance gene mi 1 leads to acquisition of virulence |
| topic | avirulence histone hairpin R gene sORF-mRNA |
| url | https://apsjournals.apsnet.org/doi/10.1094/MPMI-21-5-0576 |
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