Overexpression of the Poplar <i>WRKY51</i> Transcription Factor Enhances Salt Tolerance in <i>Arabidopsis thaliana</i>
Salt is a severe environmental stressor that affects growth and development in plants. It is significant to enhance the salt tolerance in plants. In this study, a salt-responsive WRKY transcription factor <i>PtrWRKY51</i> was isolated from <i>Populus trichocarpa</i> (clone ‘N...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-01-01
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| Series: | Forests |
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| Online Access: | https://www.mdpi.com/1999-4907/14/2/191 |
| _version_ | 1797620974305148928 |
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| author | Yangyan Zhou Qing Li Yue Zhang |
| author_facet | Yangyan Zhou Qing Li Yue Zhang |
| author_sort | Yangyan Zhou |
| collection | DOAJ |
| description | Salt is a severe environmental stressor that affects growth and development in plants. It is significant to enhance the salt tolerance in plants. In this study, a salt-responsive WRKY transcription factor <i>PtrWRKY51</i> was isolated from <i>Populus trichocarpa</i> (clone ‘Nisqually-1′). <i>PtrWRKY51</i> was highly expressed in mature leaves and root and induced by salt stress. The <i>PtrWRKY51</i> was overexpressed in <i>Arabidopsis</i> to investigate its biological functions. Compared with Col-0 lines, Overexpressed lines had an increase in germination rate of seed, root length, higher photosynthetic rate, instantaneous leaf WUE, chlorophyll content to improve salt tolerance under salt stress conditions. In contrast, compared to overexpressed and Col-0 lines, the mutant <i>wrky51</i> was more sensitive to salt stress with lower photosynthetic rate and WUE. Additionally, it was found that the complementary lines (<i>wrky51/ PtrWRKY51</i>) had almost the same salt response as Col-0. In conclusion, <i>PtrWRKY51</i> is a potential target in the enhancement of poplar tolerance by genetic engineering strategies. |
| first_indexed | 2024-03-11T08:50:09Z |
| format | Article |
| id | doaj.art-b28c7763234e46ec9e7b268a7174dd7f |
| institution | Directory Open Access Journal |
| issn | 1999-4907 |
| language | English |
| last_indexed | 2024-03-11T08:50:09Z |
| publishDate | 2023-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Forests |
| spelling | doaj.art-b28c7763234e46ec9e7b268a7174dd7f2023-11-16T20:32:33ZengMDPI AGForests1999-49072023-01-0114219110.3390/f14020191Overexpression of the Poplar <i>WRKY51</i> Transcription Factor Enhances Salt Tolerance in <i>Arabidopsis thaliana</i>Yangyan Zhou0Qing Li1Yue Zhang2School of Advanced Agricultural Sciences, Peking University, Beijing 100871, ChinaKey Research Institute of Yellow River Civilization and Sustainable Development & Collaborative Innovation Center on Yellow River Civilization Jointly Built By Henan Province and Ministry of Education, Henan University, Kaifeng 475001, ChinaGrassland Agri-Husbandry Research Center, College of Grassland Science, Qingdao Agricultural University, Qingdao 266109, ChinaSalt is a severe environmental stressor that affects growth and development in plants. It is significant to enhance the salt tolerance in plants. In this study, a salt-responsive WRKY transcription factor <i>PtrWRKY51</i> was isolated from <i>Populus trichocarpa</i> (clone ‘Nisqually-1′). <i>PtrWRKY51</i> was highly expressed in mature leaves and root and induced by salt stress. The <i>PtrWRKY51</i> was overexpressed in <i>Arabidopsis</i> to investigate its biological functions. Compared with Col-0 lines, Overexpressed lines had an increase in germination rate of seed, root length, higher photosynthetic rate, instantaneous leaf WUE, chlorophyll content to improve salt tolerance under salt stress conditions. In contrast, compared to overexpressed and Col-0 lines, the mutant <i>wrky51</i> was more sensitive to salt stress with lower photosynthetic rate and WUE. Additionally, it was found that the complementary lines (<i>wrky51/ PtrWRKY51</i>) had almost the same salt response as Col-0. In conclusion, <i>PtrWRKY51</i> is a potential target in the enhancement of poplar tolerance by genetic engineering strategies.https://www.mdpi.com/1999-4907/14/2/191poplartranscription factor<i>PtrWRKY51</i>salt tolerancephotosynthetic ratewater-use efficiency |
| spellingShingle | Yangyan Zhou Qing Li Yue Zhang Overexpression of the Poplar <i>WRKY51</i> Transcription Factor Enhances Salt Tolerance in <i>Arabidopsis thaliana</i> Forests poplar transcription factor <i>PtrWRKY51</i> salt tolerance photosynthetic rate water-use efficiency |
| title | Overexpression of the Poplar <i>WRKY51</i> Transcription Factor Enhances Salt Tolerance in <i>Arabidopsis thaliana</i> |
| title_full | Overexpression of the Poplar <i>WRKY51</i> Transcription Factor Enhances Salt Tolerance in <i>Arabidopsis thaliana</i> |
| title_fullStr | Overexpression of the Poplar <i>WRKY51</i> Transcription Factor Enhances Salt Tolerance in <i>Arabidopsis thaliana</i> |
| title_full_unstemmed | Overexpression of the Poplar <i>WRKY51</i> Transcription Factor Enhances Salt Tolerance in <i>Arabidopsis thaliana</i> |
| title_short | Overexpression of the Poplar <i>WRKY51</i> Transcription Factor Enhances Salt Tolerance in <i>Arabidopsis thaliana</i> |
| title_sort | overexpression of the poplar i wrky51 i transcription factor enhances salt tolerance in i arabidopsis thaliana i |
| topic | poplar transcription factor <i>PtrWRKY51</i> salt tolerance photosynthetic rate water-use efficiency |
| url | https://www.mdpi.com/1999-4907/14/2/191 |
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