Effects of Graphene Oxide-Gold Nanoparticles Nanocomposite on Highly Sensitive Foot-and-Mouth Disease Virus Detection
The polymerase chain reaction (PCR) has become a powerful molecular diagnostic technique over the past few decades, but remains somewhat impaired due to low specificity, poor sensitivity, and false positive results. Metal and carbon nanomaterials, quantum dots, and metal oxides, can improve the qual...
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MDPI AG
2020-09-01
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| Series: | Nanomaterials |
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| Online Access: | https://www.mdpi.com/2079-4991/10/10/1921 |
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| author | Jong-Won Kim Myeongkun Kim Kyung Kwan Lee Kwang Hyo Chung Chang-Soo Lee |
| author_facet | Jong-Won Kim Myeongkun Kim Kyung Kwan Lee Kwang Hyo Chung Chang-Soo Lee |
| author_sort | Jong-Won Kim |
| collection | DOAJ |
| description | The polymerase chain reaction (PCR) has become a powerful molecular diagnostic technique over the past few decades, but remains somewhat impaired due to low specificity, poor sensitivity, and false positive results. Metal and carbon nanomaterials, quantum dots, and metal oxides, can improve the quality and productivity of PCR assays. Here, we describe the ability of PCR assisted with nanomaterials (nano-PCR) comprising a nanocomposite of graphene oxide (GO) and gold nanoparticles (AuNPs) for sensitive detection of the foot-and-mouth disease virus (FMDV). Graphene oxide and AuNPs have been widely applied as biomedical materials for diagnosis, therapy, and drug delivery due to their unique chemical and physical properties. Foot-and-mouth disease (FMD) is highly contagious and fatal for cloven-hoofed animals including pigs, and it can thus seriously damage the swine industry. Therefore, a highly sensitive, specific, and practical method is needed to detect FMDV. The detection limit of real-time PCR improved by ~1000 fold when assisted by GO-AuNPs. We also designed a system of detecting serotypes in a single assay based on melting temperatures. Our sensitive and specific nano-PCR system can be applied to diagnose early FMDV infection, and thus may prove to be useful for clinical and biomedical applications. |
| first_indexed | 2024-03-10T16:02:01Z |
| format | Article |
| id | doaj.art-b28d5ea311d54f708797dcae3e80b48f |
| institution | Directory Open Access Journal |
| issn | 2079-4991 |
| language | English |
| last_indexed | 2024-03-10T16:02:01Z |
| publishDate | 2020-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nanomaterials |
| spelling | doaj.art-b28d5ea311d54f708797dcae3e80b48f2023-11-20T15:10:52ZengMDPI AGNanomaterials2079-49912020-09-011010192110.3390/nano10101921Effects of Graphene Oxide-Gold Nanoparticles Nanocomposite on Highly Sensitive Foot-and-Mouth Disease Virus DetectionJong-Won Kim0Myeongkun Kim1Kyung Kwan Lee2Kwang Hyo Chung3Chang-Soo Lee4Bionanotechnology Research Center, Korea Research Institute of Bioscience & Biotechnology (KRIBB) 125 Gwahak-ro, Yuseong-gu, Daejeon 34141, KoreaBionanotechnology Research Center, Korea Research Institute of Bioscience & Biotechnology (KRIBB) 125 Gwahak-ro, Yuseong-gu, Daejeon 34141, KoreaBionanotechnology Research Center, Korea Research Institute of Bioscience & Biotechnology (KRIBB) 125 Gwahak-ro, Yuseong-gu, Daejeon 34141, KoreaDignostics Platform Research Section, Electronics and Telecommunications Research Institute (ETRI) 218 Gajeong-ro, Yuseong-gu, Daejeon 34129, KoreaBionanotechnology Research Center, Korea Research Institute of Bioscience & Biotechnology (KRIBB) 125 Gwahak-ro, Yuseong-gu, Daejeon 34141, KoreaThe polymerase chain reaction (PCR) has become a powerful molecular diagnostic technique over the past few decades, but remains somewhat impaired due to low specificity, poor sensitivity, and false positive results. Metal and carbon nanomaterials, quantum dots, and metal oxides, can improve the quality and productivity of PCR assays. Here, we describe the ability of PCR assisted with nanomaterials (nano-PCR) comprising a nanocomposite of graphene oxide (GO) and gold nanoparticles (AuNPs) for sensitive detection of the foot-and-mouth disease virus (FMDV). Graphene oxide and AuNPs have been widely applied as biomedical materials for diagnosis, therapy, and drug delivery due to their unique chemical and physical properties. Foot-and-mouth disease (FMD) is highly contagious and fatal for cloven-hoofed animals including pigs, and it can thus seriously damage the swine industry. Therefore, a highly sensitive, specific, and practical method is needed to detect FMDV. The detection limit of real-time PCR improved by ~1000 fold when assisted by GO-AuNPs. We also designed a system of detecting serotypes in a single assay based on melting temperatures. Our sensitive and specific nano-PCR system can be applied to diagnose early FMDV infection, and thus may prove to be useful for clinical and biomedical applications.https://www.mdpi.com/2079-4991/10/10/1921polymerase chain reactionfoot-and-mouth disease virusgraphene oxidegold nanoparticle |
| spellingShingle | Jong-Won Kim Myeongkun Kim Kyung Kwan Lee Kwang Hyo Chung Chang-Soo Lee Effects of Graphene Oxide-Gold Nanoparticles Nanocomposite on Highly Sensitive Foot-and-Mouth Disease Virus Detection Nanomaterials polymerase chain reaction foot-and-mouth disease virus graphene oxide gold nanoparticle |
| title | Effects of Graphene Oxide-Gold Nanoparticles Nanocomposite on Highly Sensitive Foot-and-Mouth Disease Virus Detection |
| title_full | Effects of Graphene Oxide-Gold Nanoparticles Nanocomposite on Highly Sensitive Foot-and-Mouth Disease Virus Detection |
| title_fullStr | Effects of Graphene Oxide-Gold Nanoparticles Nanocomposite on Highly Sensitive Foot-and-Mouth Disease Virus Detection |
| title_full_unstemmed | Effects of Graphene Oxide-Gold Nanoparticles Nanocomposite on Highly Sensitive Foot-and-Mouth Disease Virus Detection |
| title_short | Effects of Graphene Oxide-Gold Nanoparticles Nanocomposite on Highly Sensitive Foot-and-Mouth Disease Virus Detection |
| title_sort | effects of graphene oxide gold nanoparticles nanocomposite on highly sensitive foot and mouth disease virus detection |
| topic | polymerase chain reaction foot-and-mouth disease virus graphene oxide gold nanoparticle |
| url | https://www.mdpi.com/2079-4991/10/10/1921 |
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