Transcriptomic analysis reveals key genes and pathways corresponding to Cd and Pb in the hyperaccumulator Arabis paniculata
Soil and water are increasingly at risk of contamination from the toxic heavy metals lead (Pb) and cadmium (Cd). Arabis paniculata (Brassicaceae) is a hyperaccumulator of heavy metals (HMs) found widely distributed in areas impacts by mining activities. However, the mechanism by which A. paniculata...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-04-01
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| Series: | Ecotoxicology and Environmental Safety |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0147651323002610 |
| _version_ | 1811154732343885824 |
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| author | Zhaochao Liu Lizhou Zhou Chenchen Gan Lijuan Hu Biao Pang Dan Zuo Guangyi Wang Hongcheng Wang Yingliang Liu |
| author_facet | Zhaochao Liu Lizhou Zhou Chenchen Gan Lijuan Hu Biao Pang Dan Zuo Guangyi Wang Hongcheng Wang Yingliang Liu |
| author_sort | Zhaochao Liu |
| collection | DOAJ |
| description | Soil and water are increasingly at risk of contamination from the toxic heavy metals lead (Pb) and cadmium (Cd). Arabis paniculata (Brassicaceae) is a hyperaccumulator of heavy metals (HMs) found widely distributed in areas impacts by mining activities. However, the mechanism by which A. paniculata tolerates HMs is still uncharacterized. For this experiment, we employed RNA sequencing (RNA-seq) in order to find Cd (0.25 mM)- and Pb (2.50 mM)-coresponsive genes A. paniculata. In total, 4490 and 1804 differentially expressed genes (DEGs) were identified in root tissue, and 955 and 2209 DEGs were identified in shoot tissue, after Cd and Pb exposure, respectively. Interestingly in root tissue, gene expression corresponded similarly to both Cd and Pd exposure, of which 27.48% were co-upregulated and 41.00% were co-downregulated. Kyoto Encyclopedia of Genes and Genomes (KEGG) and Gene Ontology (GO) analyses showed that the co-regulated genes were predominantly involved in transcription factors (TFs), cell wall biosynthesis, metal transport, plant hormone signal transduction, and antioxidant enzyme activity. Many critical Pb/Cd-induced DEGs involved in phytohormone biosynthesis and signal transduction, HM transport, and transcription factors were also identified. Especially the gene ABCC9 was co-downregulated in root tissues but co-upregulated in shoot tissues. The co-downregulation of ABCC9 in the roots prevented Cd and Pb from entering the vacuole rather than the cytoplasm for transporting HMs to shoots. While in shoots, the ABCC9 co-upregulated results in vacuolar Cd and Pb accumulation, which may explain why A. paniculata is a hyperaccumulator. These results will help to reveal the molecular and physiological processes underlying tolerance to HM exposure in the hyperaccumulator A. paniculata, and aid in future efforts to utilize this plant in phytoremediation. |
| first_indexed | 2024-04-10T04:22:02Z |
| format | Article |
| id | doaj.art-b28d9b4753c24da8a550551c558b495f |
| institution | Directory Open Access Journal |
| issn | 0147-6513 |
| language | English |
| last_indexed | 2024-04-10T04:22:02Z |
| publishDate | 2023-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ecotoxicology and Environmental Safety |
| spelling | doaj.art-b28d9b4753c24da8a550551c558b495f2023-03-11T04:19:12ZengElsevierEcotoxicology and Environmental Safety0147-65132023-04-01254114757Transcriptomic analysis reveals key genes and pathways corresponding to Cd and Pb in the hyperaccumulator Arabis paniculataZhaochao Liu0Lizhou Zhou1Chenchen Gan2Lijuan Hu3Biao Pang4Dan Zuo5Guangyi Wang6Hongcheng Wang7Yingliang Liu8School of Life Science, Guizhou Normal University, Guiyang 550025, Guizhou, ChinaSchool of Life Science, Guizhou Normal University, Guiyang 550025, Guizhou, ChinaSchool of Life Science, Guizhou Normal University, Guiyang 550025, Guizhou, ChinaSchool of Life Science, Guizhou Normal University, Guiyang 550025, Guizhou, ChinaSchool of Life Science, Guizhou Normal University, Guiyang 550025, Guizhou, ChinaSchool of Life Science, Guizhou Normal University, Guiyang 550025, Guizhou, ChinaSchool of Life Science, Guizhou Normal University, Guiyang 550025, Guizhou, ChinaCorresponding authors.; School of Life Science, Guizhou Normal University, Guiyang 550025, Guizhou, ChinaCorresponding authors.; School of Life Science, Guizhou Normal University, Guiyang 550025, Guizhou, ChinaSoil and water are increasingly at risk of contamination from the toxic heavy metals lead (Pb) and cadmium (Cd). Arabis paniculata (Brassicaceae) is a hyperaccumulator of heavy metals (HMs) found widely distributed in areas impacts by mining activities. However, the mechanism by which A. paniculata tolerates HMs is still uncharacterized. For this experiment, we employed RNA sequencing (RNA-seq) in order to find Cd (0.25 mM)- and Pb (2.50 mM)-coresponsive genes A. paniculata. In total, 4490 and 1804 differentially expressed genes (DEGs) were identified in root tissue, and 955 and 2209 DEGs were identified in shoot tissue, after Cd and Pb exposure, respectively. Interestingly in root tissue, gene expression corresponded similarly to both Cd and Pd exposure, of which 27.48% were co-upregulated and 41.00% were co-downregulated. Kyoto Encyclopedia of Genes and Genomes (KEGG) and Gene Ontology (GO) analyses showed that the co-regulated genes were predominantly involved in transcription factors (TFs), cell wall biosynthesis, metal transport, plant hormone signal transduction, and antioxidant enzyme activity. Many critical Pb/Cd-induced DEGs involved in phytohormone biosynthesis and signal transduction, HM transport, and transcription factors were also identified. Especially the gene ABCC9 was co-downregulated in root tissues but co-upregulated in shoot tissues. The co-downregulation of ABCC9 in the roots prevented Cd and Pb from entering the vacuole rather than the cytoplasm for transporting HMs to shoots. While in shoots, the ABCC9 co-upregulated results in vacuolar Cd and Pb accumulation, which may explain why A. paniculata is a hyperaccumulator. These results will help to reveal the molecular and physiological processes underlying tolerance to HM exposure in the hyperaccumulator A. paniculata, and aid in future efforts to utilize this plant in phytoremediation.http://www.sciencedirect.com/science/article/pii/S0147651323002610Arabis paniculataHeavy metalsTranscriptome analysisDEGsPathway analysis |
| spellingShingle | Zhaochao Liu Lizhou Zhou Chenchen Gan Lijuan Hu Biao Pang Dan Zuo Guangyi Wang Hongcheng Wang Yingliang Liu Transcriptomic analysis reveals key genes and pathways corresponding to Cd and Pb in the hyperaccumulator Arabis paniculata Ecotoxicology and Environmental Safety Arabis paniculata Heavy metals Transcriptome analysis DEGs Pathway analysis |
| title | Transcriptomic analysis reveals key genes and pathways corresponding to Cd and Pb in the hyperaccumulator Arabis paniculata |
| title_full | Transcriptomic analysis reveals key genes and pathways corresponding to Cd and Pb in the hyperaccumulator Arabis paniculata |
| title_fullStr | Transcriptomic analysis reveals key genes and pathways corresponding to Cd and Pb in the hyperaccumulator Arabis paniculata |
| title_full_unstemmed | Transcriptomic analysis reveals key genes and pathways corresponding to Cd and Pb in the hyperaccumulator Arabis paniculata |
| title_short | Transcriptomic analysis reveals key genes and pathways corresponding to Cd and Pb in the hyperaccumulator Arabis paniculata |
| title_sort | transcriptomic analysis reveals key genes and pathways corresponding to cd and pb in the hyperaccumulator arabis paniculata |
| topic | Arabis paniculata Heavy metals Transcriptome analysis DEGs Pathway analysis |
| url | http://www.sciencedirect.com/science/article/pii/S0147651323002610 |
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