Differences in Anthocyanin Accumulation Patterns and Related Gene Expression in Two Varieties of Red Pear
Red pear is a popular fruit that is appreciated for its attractive and distinctive appearance and mild flavor. In this study, we investigated the mechanism underlying the red coloration of pear skin using the ‘Xinqihong’ cultivar—which was selected as a spontaneous bud sport mutant of the ‘Xinli 7′(...
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| Format: | Article |
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MDPI AG
2021-03-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/10/4/626 |
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| author | Jianlong Liu Zhiwei Deng Hongwei Sun Jiankun Song Dingli Li Shaoling Zhang Ran Wang |
| author_facet | Jianlong Liu Zhiwei Deng Hongwei Sun Jiankun Song Dingli Li Shaoling Zhang Ran Wang |
| author_sort | Jianlong Liu |
| collection | DOAJ |
| description | Red pear is a popular fruit that is appreciated for its attractive and distinctive appearance and mild flavor. In this study, we investigated the mechanism underlying the red coloration of pear skin using the ‘Xinqihong’ cultivar—which was selected as a spontaneous bud sport mutant of the ‘Xinli 7′(<i>Pyrus betulifolia</i> Rehd.) variety and has a stronger red color that is retained in the mature fruit—as an experimental model. We compared the phenotype and gene expression patterns of the two varieties and found no significant differences at the early stage of fruit development. However, although the red color of ‘Xinli 7′ fruits began to fade 107 days after full bloom, that of ‘Xinqihong’ fruits persisted until the time of harvest. Transcriptome sequencing identified 639 genes that were differentially expressed between the two varieties, including genes related to light, calcium, and hormone signaling (e.g., <i>PbPIF3</i>, <i>PbGA2ox</i>, and the calmodulin related genes). Moreover, anthocyanin biosynthesis genes were downregulated as the red color of ‘Xinli 7′ fruits faded. These results provide insight into the molecular basis of color differences in red pears that can be useful for improving its fruit quality and commercial value. |
| first_indexed | 2024-03-10T12:54:28Z |
| format | Article |
| id | doaj.art-b28e9cd2e5204300bdd35e783625c8f5 |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-10T12:54:28Z |
| publishDate | 2021-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-b28e9cd2e5204300bdd35e783625c8f52023-11-21T12:03:43ZengMDPI AGPlants2223-77472021-03-0110462610.3390/plants10040626Differences in Anthocyanin Accumulation Patterns and Related Gene Expression in Two Varieties of Red PearJianlong Liu0Zhiwei Deng1Hongwei Sun2Jiankun Song3Dingli Li4Shaoling Zhang5Ran Wang6Lab of Pear Genetic Improvement and Germplasm Innovation, Qingdao Agricultural University, Qingdao 266109, ChinaLab of Pear Genetic Improvement and Germplasm Innovation, Qingdao Agricultural University, Qingdao 266109, ChinaLab of Pear Genetic Improvement and Germplasm Innovation, Qingdao Agricultural University, Qingdao 266109, ChinaLab of Pear Genetic Improvement and Germplasm Innovation, Qingdao Agricultural University, Qingdao 266109, ChinaLab of Pear Genetic Improvement and Germplasm Innovation, Qingdao Agricultural University, Qingdao 266109, ChinaCentre of Pear Engineering Technology Research, Nanjing Agricultural University, Nanjing 210095, ChinaLab of Pear Genetic Improvement and Germplasm Innovation, Qingdao Agricultural University, Qingdao 266109, ChinaRed pear is a popular fruit that is appreciated for its attractive and distinctive appearance and mild flavor. In this study, we investigated the mechanism underlying the red coloration of pear skin using the ‘Xinqihong’ cultivar—which was selected as a spontaneous bud sport mutant of the ‘Xinli 7′(<i>Pyrus betulifolia</i> Rehd.) variety and has a stronger red color that is retained in the mature fruit—as an experimental model. We compared the phenotype and gene expression patterns of the two varieties and found no significant differences at the early stage of fruit development. However, although the red color of ‘Xinli 7′ fruits began to fade 107 days after full bloom, that of ‘Xinqihong’ fruits persisted until the time of harvest. Transcriptome sequencing identified 639 genes that were differentially expressed between the two varieties, including genes related to light, calcium, and hormone signaling (e.g., <i>PbPIF3</i>, <i>PbGA2ox</i>, and the calmodulin related genes). Moreover, anthocyanin biosynthesis genes were downregulated as the red color of ‘Xinli 7′ fruits faded. These results provide insight into the molecular basis of color differences in red pears that can be useful for improving its fruit quality and commercial value.https://www.mdpi.com/2223-7747/10/4/626anthocyanincolorationlight signalcalciumhormone |
| spellingShingle | Jianlong Liu Zhiwei Deng Hongwei Sun Jiankun Song Dingli Li Shaoling Zhang Ran Wang Differences in Anthocyanin Accumulation Patterns and Related Gene Expression in Two Varieties of Red Pear Plants anthocyanin coloration light signal calcium hormone |
| title | Differences in Anthocyanin Accumulation Patterns and Related Gene Expression in Two Varieties of Red Pear |
| title_full | Differences in Anthocyanin Accumulation Patterns and Related Gene Expression in Two Varieties of Red Pear |
| title_fullStr | Differences in Anthocyanin Accumulation Patterns and Related Gene Expression in Two Varieties of Red Pear |
| title_full_unstemmed | Differences in Anthocyanin Accumulation Patterns and Related Gene Expression in Two Varieties of Red Pear |
| title_short | Differences in Anthocyanin Accumulation Patterns and Related Gene Expression in Two Varieties of Red Pear |
| title_sort | differences in anthocyanin accumulation patterns and related gene expression in two varieties of red pear |
| topic | anthocyanin coloration light signal calcium hormone |
| url | https://www.mdpi.com/2223-7747/10/4/626 |
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