Molecular markers for Nassella trichotoma (Poaceae) to study genetic variation in New Zealand
Premise of The Study Simple sequence repeat (SSR) markers were developed for the study of genetic diversity of New Zealand Nassella trichotoma (Poaceae) and to support future studies in its native range. Methods and Results Genomic DNA was extracted from N. trichotoma leaf material and subjected to...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2018-09-01
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| Series: | Applications in Plant Sciences |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/aps3.1181 |
| _version_ | 1811273621706899456 |
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| author | Claudia Lange Dagmar F. Goeke Graeme W. Bourdôt Shona L. Lamoureaux Ana Podolyan Gary J. Houliston |
| author_facet | Claudia Lange Dagmar F. Goeke Graeme W. Bourdôt Shona L. Lamoureaux Ana Podolyan Gary J. Houliston |
| author_sort | Claudia Lange |
| collection | DOAJ |
| description | Premise of The Study Simple sequence repeat (SSR) markers were developed for the study of genetic diversity of New Zealand Nassella trichotoma (Poaceae) and to support future studies in its native range. Methods and Results Genomic DNA was extracted from N. trichotoma leaf material and subjected to Roche 454 sequencing. From a total of 745 putative SSRs, 48 with di‐ to pentanucleotide repeats were screened, 32 primer pairs were designed, and 15 polymorphic markers were optimized for multiplex PCR on 105 N. trichotoma samples from four New Zealand regions. Each locus resulted in two to six alleles per locus, and four of the loci cross‐amplified in N. tenuissima. The mean observed and expected heterozygosity ranged from 0.00 to 0.90 and 0.00 to 0.50 per locus, respectively. Conclusions The novel SSR markers are valuable for the study of genetic diversity of N. trichotoma and might also be useful for closely related species. |
| first_indexed | 2024-04-12T23:02:44Z |
| format | Article |
| id | doaj.art-b28f0c6f80f8455aab055421859a60a1 |
| institution | Directory Open Access Journal |
| issn | 2168-0450 |
| language | English |
| last_indexed | 2024-04-12T23:02:44Z |
| publishDate | 2018-09-01 |
| publisher | Wiley |
| record_format | Article |
| series | Applications in Plant Sciences |
| spelling | doaj.art-b28f0c6f80f8455aab055421859a60a12022-12-22T03:13:00ZengWileyApplications in Plant Sciences2168-04502018-09-0169n/an/a10.1002/aps3.1181Molecular markers for Nassella trichotoma (Poaceae) to study genetic variation in New ZealandClaudia Lange0Dagmar F. Goeke1Graeme W. Bourdôt2Shona L. Lamoureaux3Ana Podolyan4Gary J. Houliston5Manaaki Whenua Landcare Research 54 Gerald Street Lincoln 7608 New ZealandScion, 49 Sala Street Rotorua 3010 New ZealandAgResearch 1365 Springs Road Lincoln 7674 New ZealandAgResearch 1365 Springs Road Lincoln 7674 New ZealandManaaki Whenua Landcare Research 54 Gerald Street Lincoln 7608 New ZealandManaaki Whenua Landcare Research 54 Gerald Street Lincoln 7608 New ZealandPremise of The Study Simple sequence repeat (SSR) markers were developed for the study of genetic diversity of New Zealand Nassella trichotoma (Poaceae) and to support future studies in its native range. Methods and Results Genomic DNA was extracted from N. trichotoma leaf material and subjected to Roche 454 sequencing. From a total of 745 putative SSRs, 48 with di‐ to pentanucleotide repeats were screened, 32 primer pairs were designed, and 15 polymorphic markers were optimized for multiplex PCR on 105 N. trichotoma samples from four New Zealand regions. Each locus resulted in two to six alleles per locus, and four of the loci cross‐amplified in N. tenuissima. The mean observed and expected heterozygosity ranged from 0.00 to 0.90 and 0.00 to 0.50 per locus, respectively. Conclusions The novel SSR markers are valuable for the study of genetic diversity of N. trichotoma and might also be useful for closely related species.https://doi.org/10.1002/aps3.1181Nassella tenuissimaNassella trichotomanassella tussockNew ZealandPoaceaesimple sequence repeat (SSR) markers |
| spellingShingle | Claudia Lange Dagmar F. Goeke Graeme W. Bourdôt Shona L. Lamoureaux Ana Podolyan Gary J. Houliston Molecular markers for Nassella trichotoma (Poaceae) to study genetic variation in New Zealand Applications in Plant Sciences Nassella tenuissima Nassella trichotoma nassella tussock New Zealand Poaceae simple sequence repeat (SSR) markers |
| title | Molecular markers for Nassella trichotoma (Poaceae) to study genetic variation in New Zealand |
| title_full | Molecular markers for Nassella trichotoma (Poaceae) to study genetic variation in New Zealand |
| title_fullStr | Molecular markers for Nassella trichotoma (Poaceae) to study genetic variation in New Zealand |
| title_full_unstemmed | Molecular markers for Nassella trichotoma (Poaceae) to study genetic variation in New Zealand |
| title_short | Molecular markers for Nassella trichotoma (Poaceae) to study genetic variation in New Zealand |
| title_sort | molecular markers for nassella trichotoma poaceae to study genetic variation in new zealand |
| topic | Nassella tenuissima Nassella trichotoma nassella tussock New Zealand Poaceae simple sequence repeat (SSR) markers |
| url | https://doi.org/10.1002/aps3.1181 |
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