Characterization of Phase I and Glucuronide Phase II Metabolites of 17 Mycotoxins Using Liquid Chromatography—High-Resolution Mass Spectrometry
Routine mycotoxin biomonitoring methods do not include many mycotoxin phase I and phase II metabolites, which may significantly underestimate mycotoxin exposure especially for heavily metabolized mycotoxins. Additional research efforts are also needed to measure metabolites in vivo after exposure an...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2019-07-01
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| Series: | Toxins |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2072-6651/11/8/433 |
| _version_ | 1811279119389818880 |
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| author | Irina Slobodchikova Reajean Sivakumar Md Samiur Rahman Dajana Vuckovic |
| author_facet | Irina Slobodchikova Reajean Sivakumar Md Samiur Rahman Dajana Vuckovic |
| author_sort | Irina Slobodchikova |
| collection | DOAJ |
| description | Routine mycotoxin biomonitoring methods do not include many mycotoxin phase I and phase II metabolites, which may significantly underestimate mycotoxin exposure especially for heavily metabolized mycotoxins. Additional research efforts are also needed to measure metabolites in vivo after exposure and to establish which mycotoxin metabolites should be prioritized for the inclusion during large-scale biomonitoring efforts. The objective of this study was to perform human in vitro microsomal incubations of 17 mycotoxins and systematically characterize all resulting metabolites using liquid chromatography−high-resolution mass spectrometry (LC-HRMS). The results obtained were then used to build a comprehensive LC-MS library and expand a validated 17-mycotoxin method for exposure monitoring to screening of additional 188 metabolites, including 100 metabolites reported for the first time. The final method represents one of the most comprehensive LC-HRMS methods for mycotoxin biomonitoring or metabolism/fate studies. |
| first_indexed | 2024-04-13T00:49:02Z |
| format | Article |
| id | doaj.art-b28f63085543428c84d4a87d11843ba7 |
| institution | Directory Open Access Journal |
| issn | 2072-6651 |
| language | English |
| last_indexed | 2024-04-13T00:49:02Z |
| publishDate | 2019-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Toxins |
| spelling | doaj.art-b28f63085543428c84d4a87d11843ba72022-12-22T03:09:55ZengMDPI AGToxins2072-66512019-07-0111843310.3390/toxins11080433toxins11080433Characterization of Phase I and Glucuronide Phase II Metabolites of 17 Mycotoxins Using Liquid Chromatography—High-Resolution Mass SpectrometryIrina Slobodchikova0Reajean Sivakumar1Md Samiur Rahman2Dajana Vuckovic3Department of Chemistry and Biochemistry, Concordia University, 7141 Sherbrooke Street West, Montreal, QC H4B 1R6, CanadaDepartment of Chemistry and Biochemistry, Concordia University, 7141 Sherbrooke Street West, Montreal, QC H4B 1R6, CanadaDepartment of Chemistry and Biochemistry, Concordia University, 7141 Sherbrooke Street West, Montreal, QC H4B 1R6, CanadaDepartment of Chemistry and Biochemistry, Concordia University, 7141 Sherbrooke Street West, Montreal, QC H4B 1R6, CanadaRoutine mycotoxin biomonitoring methods do not include many mycotoxin phase I and phase II metabolites, which may significantly underestimate mycotoxin exposure especially for heavily metabolized mycotoxins. Additional research efforts are also needed to measure metabolites in vivo after exposure and to establish which mycotoxin metabolites should be prioritized for the inclusion during large-scale biomonitoring efforts. The objective of this study was to perform human in vitro microsomal incubations of 17 mycotoxins and systematically characterize all resulting metabolites using liquid chromatography−high-resolution mass spectrometry (LC-HRMS). The results obtained were then used to build a comprehensive LC-MS library and expand a validated 17-mycotoxin method for exposure monitoring to screening of additional 188 metabolites, including 100 metabolites reported for the first time. The final method represents one of the most comprehensive LC-HRMS methods for mycotoxin biomonitoring or metabolism/fate studies.https://www.mdpi.com/2072-6651/11/8/433mycotoxinsmetabolismhigh-resolution mass spectrometry (HRMS)microsomal incubationglucuronidationhumanbiomonitoring |
| spellingShingle | Irina Slobodchikova Reajean Sivakumar Md Samiur Rahman Dajana Vuckovic Characterization of Phase I and Glucuronide Phase II Metabolites of 17 Mycotoxins Using Liquid Chromatography—High-Resolution Mass Spectrometry Toxins mycotoxins metabolism high-resolution mass spectrometry (HRMS) microsomal incubation glucuronidation human biomonitoring |
| title | Characterization of Phase I and Glucuronide Phase II Metabolites of 17 Mycotoxins Using Liquid Chromatography—High-Resolution Mass Spectrometry |
| title_full | Characterization of Phase I and Glucuronide Phase II Metabolites of 17 Mycotoxins Using Liquid Chromatography—High-Resolution Mass Spectrometry |
| title_fullStr | Characterization of Phase I and Glucuronide Phase II Metabolites of 17 Mycotoxins Using Liquid Chromatography—High-Resolution Mass Spectrometry |
| title_full_unstemmed | Characterization of Phase I and Glucuronide Phase II Metabolites of 17 Mycotoxins Using Liquid Chromatography—High-Resolution Mass Spectrometry |
| title_short | Characterization of Phase I and Glucuronide Phase II Metabolites of 17 Mycotoxins Using Liquid Chromatography—High-Resolution Mass Spectrometry |
| title_sort | characterization of phase i and glucuronide phase ii metabolites of 17 mycotoxins using liquid chromatography high resolution mass spectrometry |
| topic | mycotoxins metabolism high-resolution mass spectrometry (HRMS) microsomal incubation glucuronidation human biomonitoring |
| url | https://www.mdpi.com/2072-6651/11/8/433 |
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