Bisphenol A triggers apoptosis in mouse pre-antral follicle granulosa cells via oxidative stress
Abstract Background Bisphenol A (BPA), an endocrine disrupting chemical with weak estrogenic and anti-androgenic activity, is widely present in various environmental media and organisms. It has certain reproductive toxicity and can cause a variety of female reproductive system diseases. Although BPA...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2024-01-01
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| Series: | Journal of Ovarian Research |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13048-023-01322-y |
| _version_ | 1797349801556180992 |
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| author | Chen Wang Chaofan He Shumin Xu Yuanyuan Gao Kaixian Wang Meng Liang Ke Hu |
| author_facet | Chen Wang Chaofan He Shumin Xu Yuanyuan Gao Kaixian Wang Meng Liang Ke Hu |
| author_sort | Chen Wang |
| collection | DOAJ |
| description | Abstract Background Bisphenol A (BPA), an endocrine disrupting chemical with weak estrogenic and anti-androgenic activity, is widely present in various environmental media and organisms. It has certain reproductive toxicity and can cause a variety of female reproductive system diseases. Although BPA-stimulated apoptosis of granulosa cells has been widely elaborated, the effect of BPA on mouse pre-antral follicle granulosa cells (mpGCs) has not been well elucidated. Results In this study, the results of live-dead cell staining showed that high concentrations of BPA severely impaired mpGCs growth viability and affected the cell cycle transition of mpGCs. We confirmed that BPA promotes the production of reactive oxygen species (ROS) and facilitates oxidative stress in mpGCs. In addition, immunofluorescence, transmission electron microscopy, and flow cytometry experiments demonstrated that BPA treatment for mpGCs resulted in apoptotic features, such as rounding, cytoplasmic crinkling, and mitochondrial damage. This was accompanied by a large production of ROS and apoptosis-inducing factor (AIF) translocation from the mitochondria to the nucleus. RNA-seq data showed that several apoptosis-related pathways were enriched in the high concentration BPA-treated group compared with the normal group, such as the p53 pathway, MAPK pathway, etc. Conclusions These results suggest that cells undergo oxidative stress effects and apoptosis after BPA treatment for mpGCs, which affects normal follicle development. The potential mechanism of BPA-induced female reproductive toxicity was elucidated, while providing a research basis for the prevention and treatment of female reproductive diseases. |
| first_indexed | 2024-03-08T12:35:41Z |
| format | Article |
| id | doaj.art-b29070166a734e818969e157471bdd26 |
| institution | Directory Open Access Journal |
| issn | 1757-2215 |
| language | English |
| last_indexed | 2024-03-08T12:35:41Z |
| publishDate | 2024-01-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Ovarian Research |
| spelling | doaj.art-b29070166a734e818969e157471bdd262024-01-21T12:30:36ZengBMCJournal of Ovarian Research1757-22152024-01-0117111110.1186/s13048-023-01322-yBisphenol A triggers apoptosis in mouse pre-antral follicle granulosa cells via oxidative stressChen Wang0Chaofan He1Shumin Xu2Yuanyuan Gao3Kaixian Wang4Meng Liang5Ke Hu6School of Life Science, Bengbu Medical UniversitySchool of Life Science, Bengbu Medical UniversitySchool of Life Science, Bengbu Medical UniversitySchool of Life Science, Bengbu Medical UniversitySchool of Life Science, Bengbu Medical UniversitySchool of Life Science, Bengbu Medical UniversitySchool of Life Science, Bengbu Medical UniversityAbstract Background Bisphenol A (BPA), an endocrine disrupting chemical with weak estrogenic and anti-androgenic activity, is widely present in various environmental media and organisms. It has certain reproductive toxicity and can cause a variety of female reproductive system diseases. Although BPA-stimulated apoptosis of granulosa cells has been widely elaborated, the effect of BPA on mouse pre-antral follicle granulosa cells (mpGCs) has not been well elucidated. Results In this study, the results of live-dead cell staining showed that high concentrations of BPA severely impaired mpGCs growth viability and affected the cell cycle transition of mpGCs. We confirmed that BPA promotes the production of reactive oxygen species (ROS) and facilitates oxidative stress in mpGCs. In addition, immunofluorescence, transmission electron microscopy, and flow cytometry experiments demonstrated that BPA treatment for mpGCs resulted in apoptotic features, such as rounding, cytoplasmic crinkling, and mitochondrial damage. This was accompanied by a large production of ROS and apoptosis-inducing factor (AIF) translocation from the mitochondria to the nucleus. RNA-seq data showed that several apoptosis-related pathways were enriched in the high concentration BPA-treated group compared with the normal group, such as the p53 pathway, MAPK pathway, etc. Conclusions These results suggest that cells undergo oxidative stress effects and apoptosis after BPA treatment for mpGCs, which affects normal follicle development. The potential mechanism of BPA-induced female reproductive toxicity was elucidated, while providing a research basis for the prevention and treatment of female reproductive diseases.https://doi.org/10.1186/s13048-023-01322-yBisphenol AmpGCsReactive oxygen speciesOxidative stressApoptosis |
| spellingShingle | Chen Wang Chaofan He Shumin Xu Yuanyuan Gao Kaixian Wang Meng Liang Ke Hu Bisphenol A triggers apoptosis in mouse pre-antral follicle granulosa cells via oxidative stress Journal of Ovarian Research Bisphenol A mpGCs Reactive oxygen species Oxidative stress Apoptosis |
| title | Bisphenol A triggers apoptosis in mouse pre-antral follicle granulosa cells via oxidative stress |
| title_full | Bisphenol A triggers apoptosis in mouse pre-antral follicle granulosa cells via oxidative stress |
| title_fullStr | Bisphenol A triggers apoptosis in mouse pre-antral follicle granulosa cells via oxidative stress |
| title_full_unstemmed | Bisphenol A triggers apoptosis in mouse pre-antral follicle granulosa cells via oxidative stress |
| title_short | Bisphenol A triggers apoptosis in mouse pre-antral follicle granulosa cells via oxidative stress |
| title_sort | bisphenol a triggers apoptosis in mouse pre antral follicle granulosa cells via oxidative stress |
| topic | Bisphenol A mpGCs Reactive oxygen species Oxidative stress Apoptosis |
| url | https://doi.org/10.1186/s13048-023-01322-y |
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