Bioactivity of nanosilver in Caenorhabditis elegans: Effects of size, coat, and shape
The in vivo toxicity to eukaryotes of nanosilver (AgNP) spheres and plates in two sizes each was assessed using the simple model organism Caenorhabditis elegans. For each shape, smaller AgNP size correlated with higher toxicity, as indicated by reduced larval growth. Smaller size also correlated wit...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2014-01-01
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| Series: | Toxicology Reports |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2214750014001176 |
| _version_ | 1823938125101006848 |
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| author | Piper Reid Hunt Zachary Keltner Xiugong Gao Steven J. Oldenburg Priyanka Bushana Nicholas Olejnik Robert L. Sprando |
| author_facet | Piper Reid Hunt Zachary Keltner Xiugong Gao Steven J. Oldenburg Priyanka Bushana Nicholas Olejnik Robert L. Sprando |
| author_sort | Piper Reid Hunt |
| collection | DOAJ |
| description | The in vivo toxicity to eukaryotes of nanosilver (AgNP) spheres and plates in two sizes each was assessed using the simple model organism Caenorhabditis elegans. For each shape, smaller AgNP size correlated with higher toxicity, as indicated by reduced larval growth. Smaller size also correlated with significant increases in silver uptake for silver nanospheres. Citrate coated silver spheres of 20 nm diameter induced an innate immune response that increased or held steady over 24 h, while regulation of genes involved in metal metabolism peaked at 4 h and subsequently decreased. For AgNP spheres, coating altered bioactivity, with a toxicity ranking of polyethylene glycol (PEG) > polyvinylpyrrolidone (PVP) ≅ branched polyethyleneimine (BPEI) > citrate, but silver uptake ranking of PEG > PVP > citrate > BPEI. Our findings in C. elegans correlate well with findings in rodents for AgNP size vs. uptake and toxicity, as well as for induction of immune effectors, while using methods that are faster and far less expensive, supporting the use of C. elegans as an alternative model for early toxicity screening. |
| first_indexed | 2024-12-17T02:40:55Z |
| format | Article |
| id | doaj.art-b293b4e1626d437e9590c11752dab68b |
| institution | Directory Open Access Journal |
| issn | 2214-7500 |
| language | English |
| last_indexed | 2024-12-17T02:40:55Z |
| publishDate | 2014-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Toxicology Reports |
| spelling | doaj.art-b293b4e1626d437e9590c11752dab68b2022-12-21T22:06:43ZengElsevierToxicology Reports2214-75002014-01-011C92394410.1016/j.toxrep.2014.10.020Bioactivity of nanosilver in Caenorhabditis elegans: Effects of size, coat, and shapePiper Reid Hunt0Zachary Keltner1Xiugong Gao2Steven J. Oldenburg3Priyanka Bushana4Nicholas Olejnik5Robert L. Sprando6Center for Food Safety and Applied Nutrition, FDA, Laurel, MD, United StatesCenter for Food Safety and Applied Nutrition, FDA, Laurel, MD, United StatesCenter for Food Safety and Applied Nutrition, FDA, Laurel, MD, United StatesnanoComposix Inc., San Diego, CA, United StatesCenter for Food Safety and Applied Nutrition, FDA, Laurel, MD, United StatesCenter for Food Safety and Applied Nutrition, FDA, Laurel, MD, United StatesCenter for Food Safety and Applied Nutrition, FDA, Laurel, MD, United StatesThe in vivo toxicity to eukaryotes of nanosilver (AgNP) spheres and plates in two sizes each was assessed using the simple model organism Caenorhabditis elegans. For each shape, smaller AgNP size correlated with higher toxicity, as indicated by reduced larval growth. Smaller size also correlated with significant increases in silver uptake for silver nanospheres. Citrate coated silver spheres of 20 nm diameter induced an innate immune response that increased or held steady over 24 h, while regulation of genes involved in metal metabolism peaked at 4 h and subsequently decreased. For AgNP spheres, coating altered bioactivity, with a toxicity ranking of polyethylene glycol (PEG) > polyvinylpyrrolidone (PVP) ≅ branched polyethyleneimine (BPEI) > citrate, but silver uptake ranking of PEG > PVP > citrate > BPEI. Our findings in C. elegans correlate well with findings in rodents for AgNP size vs. uptake and toxicity, as well as for induction of immune effectors, while using methods that are faster and far less expensive, supporting the use of C. elegans as an alternative model for early toxicity screening.http://www.sciencedirect.com/science/article/pii/S2214750014001176NanosilverIonic silverGrowth assaySilver uptakePhysico-chemical propertiesAlternative toxicity model |
| spellingShingle | Piper Reid Hunt Zachary Keltner Xiugong Gao Steven J. Oldenburg Priyanka Bushana Nicholas Olejnik Robert L. Sprando Bioactivity of nanosilver in Caenorhabditis elegans: Effects of size, coat, and shape Toxicology Reports Nanosilver Ionic silver Growth assay Silver uptake Physico-chemical properties Alternative toxicity model |
| title | Bioactivity of nanosilver in Caenorhabditis elegans: Effects of size, coat, and shape |
| title_full | Bioactivity of nanosilver in Caenorhabditis elegans: Effects of size, coat, and shape |
| title_fullStr | Bioactivity of nanosilver in Caenorhabditis elegans: Effects of size, coat, and shape |
| title_full_unstemmed | Bioactivity of nanosilver in Caenorhabditis elegans: Effects of size, coat, and shape |
| title_short | Bioactivity of nanosilver in Caenorhabditis elegans: Effects of size, coat, and shape |
| title_sort | bioactivity of nanosilver in caenorhabditis elegans effects of size coat and shape |
| topic | Nanosilver Ionic silver Growth assay Silver uptake Physico-chemical properties Alternative toxicity model |
| url | http://www.sciencedirect.com/science/article/pii/S2214750014001176 |
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