Analysis of MTHFR Gene C.677C>T and C.1298A>C Polymorphisms in Iranian Patients with Non-Syndromic Cleft Lip and Palate.
Non-syndromic cleft lip with or without cleft palate (nsCL/P) is one of the most common congenital abnormalities of the orofacial region with a multifactorial etiology. The present study aimed to investigate the association of two common polymorphisms of methylenetetrahydrofolate reductase (MTHFR) g...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Tehran University of Medical Sciences
2014-06-01
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| Series: | Iranian Journal of Public Health |
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| Online Access: | https://ijph.tums.ac.ir/index.php/ijph/article/view/4054 |
| _version_ | 1819058836311375872 |
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| author | Arezoo Jahanbin Nadia Hasanzadeh Faraneh Abdolhoseinpour Ariane Sadr-Nabavi Mohammad-Ali Raisolsadat Khosro Shamsian Farnaz Mohajertehran Hamidreza Kianifar |
| author_facet | Arezoo Jahanbin Nadia Hasanzadeh Faraneh Abdolhoseinpour Ariane Sadr-Nabavi Mohammad-Ali Raisolsadat Khosro Shamsian Farnaz Mohajertehran Hamidreza Kianifar |
| author_sort | Arezoo Jahanbin |
| collection | DOAJ |
| description | Non-syndromic cleft lip with or without cleft palate (nsCL/P) is one of the most common congenital abnormalities of the orofacial region with a multifactorial etiology. The present study aimed to investigate the association of two common polymorphisms of methylenetetrahydrofolate reductase (MTHFR) gene (c.677C>T and c.1298A>C) with the occurrence of nsCL/P in an Iranian population.Forty-five nsCL/P patients, 43 mothers of patients, and 101 unrelated controls participated in the present study. Analysis of c.677C>T and c.1298A>C polymorphisms in MTHFR gene was conducted using polymerase chain reaction and restriction enzyme digestions.There was no statistical difference in genotype and allele frequencies for c.677C>T variants between patients or their mothers and the control group. However, differences in the frequencies of alleles and genotypes of c.1298A>C polymorphism were statistically significant between patients and control group (P=0.01 for alleles and P=0.005 for genotypes). The odds ratios (OR) for the CC versus AA homozygotes were 6.1 (95% CI 1.8-20.5) and 4.2 (95% CI 1.1-15.4), in patients and mothers, respectively.We found no association between genetic polymorphism of MTHFR c.677C>T and the risk of nsCL/P in the population studied. Yet the results suggested that c.1298A>C polymorphism of MTHFR gene may be a risk factor for the occurrence of nsCL/P in the Iranian population. |
| first_indexed | 2024-12-21T14:01:32Z |
| format | Article |
| id | doaj.art-b29b382115b44067b1767acd649cb38e |
| institution | Directory Open Access Journal |
| issn | 2251-6085 2251-6093 |
| language | English |
| last_indexed | 2024-12-21T14:01:32Z |
| publishDate | 2014-06-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Public Health |
| spelling | doaj.art-b29b382115b44067b1767acd649cb38e2022-12-21T19:01:21ZengTehran University of Medical SciencesIranian Journal of Public Health2251-60852251-60932014-06-014363909Analysis of MTHFR Gene C.677C>T and C.1298A>C Polymorphisms in Iranian Patients with Non-Syndromic Cleft Lip and Palate.Arezoo Jahanbin0Nadia Hasanzadeh1Faraneh Abdolhoseinpour2Ariane Sadr-Nabavi3Mohammad-Ali Raisolsadat4Khosro Shamsian5Farnaz Mohajertehran6Hamidreza Kianifar71. Dept. of Orthodontics, School of Dentistry, Mashhad University of Medical Sciences , Mashhad, Iran.2. Dental Material Research Center, School of Dentistry, Mashhad University of Medical Sciences , Mashhad, Iran.3. Mashhad University of Medical Sciences , Mashhad, Iran.4. Dept. of Medical Genetics, School of Medicine, Mashhad University of Medical Sciences , Mashhad, Iran.5. Dr. Sheikh Hospital, Mashhad University of Medical Sciences , Mashhad, Iran.6. Jahade Daneshgahi of Mashhad , Mashhad, Iran.7. Dental Research Center, Mashhad University of Medical Sciences , Mashhad, Iran.8. Dept. of Pediatric Gastroenterology, Ghaem Medical Center, Mashhad University of Medical Sciences , Mashhad, Iran.Non-syndromic cleft lip with or without cleft palate (nsCL/P) is one of the most common congenital abnormalities of the orofacial region with a multifactorial etiology. The present study aimed to investigate the association of two common polymorphisms of methylenetetrahydrofolate reductase (MTHFR) gene (c.677C>T and c.1298A>C) with the occurrence of nsCL/P in an Iranian population.Forty-five nsCL/P patients, 43 mothers of patients, and 101 unrelated controls participated in the present study. Analysis of c.677C>T and c.1298A>C polymorphisms in MTHFR gene was conducted using polymerase chain reaction and restriction enzyme digestions.There was no statistical difference in genotype and allele frequencies for c.677C>T variants between patients or their mothers and the control group. However, differences in the frequencies of alleles and genotypes of c.1298A>C polymorphism were statistically significant between patients and control group (P=0.01 for alleles and P=0.005 for genotypes). The odds ratios (OR) for the CC versus AA homozygotes were 6.1 (95% CI 1.8-20.5) and 4.2 (95% CI 1.1-15.4), in patients and mothers, respectively.We found no association between genetic polymorphism of MTHFR c.677C>T and the risk of nsCL/P in the population studied. Yet the results suggested that c.1298A>C polymorphism of MTHFR gene may be a risk factor for the occurrence of nsCL/P in the Iranian population.https://ijph.tums.ac.ir/index.php/ijph/article/view/4054Cleft LipCleft palateFolic acidMTHFRMethylenetetrahydrofolate reductaseNon-syndromic cleft |
| spellingShingle | Arezoo Jahanbin Nadia Hasanzadeh Faraneh Abdolhoseinpour Ariane Sadr-Nabavi Mohammad-Ali Raisolsadat Khosro Shamsian Farnaz Mohajertehran Hamidreza Kianifar Analysis of MTHFR Gene C.677C>T and C.1298A>C Polymorphisms in Iranian Patients with Non-Syndromic Cleft Lip and Palate. Iranian Journal of Public Health Cleft Lip Cleft palate Folic acid MTHFR Methylenetetrahydrofolate reductase Non-syndromic cleft |
| title | Analysis of MTHFR Gene C.677C>T and C.1298A>C Polymorphisms in Iranian Patients with Non-Syndromic Cleft Lip and Palate. |
| title_full | Analysis of MTHFR Gene C.677C>T and C.1298A>C Polymorphisms in Iranian Patients with Non-Syndromic Cleft Lip and Palate. |
| title_fullStr | Analysis of MTHFR Gene C.677C>T and C.1298A>C Polymorphisms in Iranian Patients with Non-Syndromic Cleft Lip and Palate. |
| title_full_unstemmed | Analysis of MTHFR Gene C.677C>T and C.1298A>C Polymorphisms in Iranian Patients with Non-Syndromic Cleft Lip and Palate. |
| title_short | Analysis of MTHFR Gene C.677C>T and C.1298A>C Polymorphisms in Iranian Patients with Non-Syndromic Cleft Lip and Palate. |
| title_sort | analysis of mthfr gene c 677c t and c 1298a c polymorphisms in iranian patients with non syndromic cleft lip and palate |
| topic | Cleft Lip Cleft palate Folic acid MTHFR Methylenetetrahydrofolate reductase Non-syndromic cleft |
| url | https://ijph.tums.ac.ir/index.php/ijph/article/view/4054 |
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