Effect of aging on mitochondria and metabolism of bovine granulosa cells
This study investigated the effect of aging on mitochondria in granulosa cells (GCs) collected from the antral follicles of young and aged cows (25–50 months and over 140 months in age, respectively). When GCs were cultured under 20% O2 for 4 days, mitochondrial DNA copy number (Mt-number), determin...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
The Society for Reproduction and Development
2020-09-01
|
| Series: | The Journal of Reproduction and Development |
| Subjects: | |
| Online Access: | https://www.jstage.jst.go.jp/article/jrd/66/6/66_2020-071/_pdf/-char/en |
| _version_ | 1797615403119149056 |
|---|---|
| author | Shuta NAGATA Kaoru TATEMATSU Kazuki KANSAKU Yuki INOUE Mitsuru KOBAYASHI Koumei SHIRASUNA Hisataka IWATA |
| author_facet | Shuta NAGATA Kaoru TATEMATSU Kazuki KANSAKU Yuki INOUE Mitsuru KOBAYASHI Koumei SHIRASUNA Hisataka IWATA |
| author_sort | Shuta NAGATA |
| collection | DOAJ |
| description | This study investigated the effect of aging on mitochondria in granulosa cells (GCs) collected from the antral follicles of young and aged cows (25–50 months and over 140 months in age, respectively). When GCs were cultured under 20% O2 for 4 days, mitochondrial DNA copy number (Mt-number), determined by real-time PCR, increased throughout the culture period, and the extent of increase was greater in the GCs of young cows than in those of old cows. In a second experiment, GCs were cultured under 20% O2 for 24 h. Protein levels of TOMM20 and TFAM in GCs were lower in aged cows than in young cows, and the amount of reactive oxygen species and the mitochondrial membrane potential were higher, whereas ATP content and proliferation activity were lower, respectively. Glucose consumption and lactate production were higher in the GCs of aged cows than in those of young cows. When GCs were cultured under 5% or 20% O2 for 24 h, low O2 decreased ATP content and increased glucose consumption in GCs of both age groups compared with high O2; however, low O2 decreased the Mt-number only in the GCs of young cows. In conclusion, we show that aging affects mitochondrial quantity, function, and response to differential O2 tensions in GCs. |
| first_indexed | 2024-03-11T07:25:47Z |
| format | Article |
| id | doaj.art-b29ba7a92f0b480683cf865258029f1d |
| institution | Directory Open Access Journal |
| issn | 0916-8818 1348-4400 |
| language | English |
| last_indexed | 2024-03-11T07:25:47Z |
| publishDate | 2020-09-01 |
| publisher | The Society for Reproduction and Development |
| record_format | Article |
| series | The Journal of Reproduction and Development |
| spelling | doaj.art-b29ba7a92f0b480683cf865258029f1d2023-11-17T07:40:15ZengThe Society for Reproduction and DevelopmentThe Journal of Reproduction and Development0916-88181348-44002020-09-0166654755410.1262/jrd.2020-071jrdEffect of aging on mitochondria and metabolism of bovine granulosa cellsShuta NAGATA0Kaoru TATEMATSU1Kazuki KANSAKU2Yuki INOUE3Mitsuru KOBAYASHI4Koumei SHIRASUNA5Hisataka IWATA6Department of Animal Science, Tokyo University of Agriculture Department of Animal Science, Kanagawa 243-0034, JapanDepartment of Animal Science, Tokyo University of Agriculture Department of Animal Science, Kanagawa 243-0034, JapanDepartment of Animal Science, Tokyo University of Agriculture Department of Animal Science, Kanagawa 243-0034, JapanDepartment of Animal Science, Tokyo University of Agriculture Department of Animal Science, Kanagawa 243-0034, JapanDepartment of Animal Science, Tokyo University of Agriculture Department of Animal Science, Kanagawa 243-0034, JapanDepartment of Animal Science, Tokyo University of Agriculture Department of Animal Science, Kanagawa 243-0034, JapanDepartment of Animal Science, Tokyo University of Agriculture Department of Animal Science, Kanagawa 243-0034, JapanThis study investigated the effect of aging on mitochondria in granulosa cells (GCs) collected from the antral follicles of young and aged cows (25–50 months and over 140 months in age, respectively). When GCs were cultured under 20% O2 for 4 days, mitochondrial DNA copy number (Mt-number), determined by real-time PCR, increased throughout the culture period, and the extent of increase was greater in the GCs of young cows than in those of old cows. In a second experiment, GCs were cultured under 20% O2 for 24 h. Protein levels of TOMM20 and TFAM in GCs were lower in aged cows than in young cows, and the amount of reactive oxygen species and the mitochondrial membrane potential were higher, whereas ATP content and proliferation activity were lower, respectively. Glucose consumption and lactate production were higher in the GCs of aged cows than in those of young cows. When GCs were cultured under 5% or 20% O2 for 24 h, low O2 decreased ATP content and increased glucose consumption in GCs of both age groups compared with high O2; however, low O2 decreased the Mt-number only in the GCs of young cows. In conclusion, we show that aging affects mitochondrial quantity, function, and response to differential O2 tensions in GCs.https://www.jstage.jst.go.jp/article/jrd/66/6/66_2020-071/_pdf/-char/enagingcowgranulosa cellsmitochondria |
| spellingShingle | Shuta NAGATA Kaoru TATEMATSU Kazuki KANSAKU Yuki INOUE Mitsuru KOBAYASHI Koumei SHIRASUNA Hisataka IWATA Effect of aging on mitochondria and metabolism of bovine granulosa cells The Journal of Reproduction and Development aging cow granulosa cells mitochondria |
| title | Effect of aging on mitochondria and metabolism of bovine granulosa cells |
| title_full | Effect of aging on mitochondria and metabolism of bovine granulosa cells |
| title_fullStr | Effect of aging on mitochondria and metabolism of bovine granulosa cells |
| title_full_unstemmed | Effect of aging on mitochondria and metabolism of bovine granulosa cells |
| title_short | Effect of aging on mitochondria and metabolism of bovine granulosa cells |
| title_sort | effect of aging on mitochondria and metabolism of bovine granulosa cells |
| topic | aging cow granulosa cells mitochondria |
| url | https://www.jstage.jst.go.jp/article/jrd/66/6/66_2020-071/_pdf/-char/en |
| work_keys_str_mv | AT shutanagata effectofagingonmitochondriaandmetabolismofbovinegranulosacells AT kaorutatematsu effectofagingonmitochondriaandmetabolismofbovinegranulosacells AT kazukikansaku effectofagingonmitochondriaandmetabolismofbovinegranulosacells AT yukiinoue effectofagingonmitochondriaandmetabolismofbovinegranulosacells AT mitsurukobayashi effectofagingonmitochondriaandmetabolismofbovinegranulosacells AT koumeishirasuna effectofagingonmitochondriaandmetabolismofbovinegranulosacells AT hisatakaiwata effectofagingonmitochondriaandmetabolismofbovinegranulosacells |