Effects of diode laser application on inflammation and mpo in periodontal tissues in a rat model
Abstract Objective In this study, we aimed to histologically and immunologically evaluate the effect of diode laser treatment when applied adjunctive to scaling and root planing (SRP) in an experimental periodontitis model. Materials and methods We used Wistar-Albino rats (n=60) with average wei...
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| Format: | Article |
| Language: | English |
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University of São Paulo
2018-07-01
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| Series: | Journal of Applied Oral Science |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-77572018000100483&lng=en&tlng=en |
| _version_ | 1818477950411997184 |
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| author | Mustafa Özay USLU Abubekir ELTAS İsmail MARAKOĞLU Serkan DÜNDAR Kazım ŞAHIN İbrahim Hanifi ÖZERCAN |
| author_facet | Mustafa Özay USLU Abubekir ELTAS İsmail MARAKOĞLU Serkan DÜNDAR Kazım ŞAHIN İbrahim Hanifi ÖZERCAN |
| author_sort | Mustafa Özay USLU |
| collection | DOAJ |
| description | Abstract Objective In this study, we aimed to histologically and immunologically evaluate the effect of diode laser treatment when applied adjunctive to scaling and root planing (SRP) in an experimental periodontitis model. Materials and methods We used Wistar-Albino rats (n=60) with average weight of 230 g. Experimental periodontitis was induced by ligature at the right and left first mandibular molar teeth in all rats. After 11 days, the ligature was removed and rats were divided into two groups. The control group (n=30) received only SRP treatment, while the laser group (n=30) received a diode laser (GaAlAs, 810 nm, 1 W, 10 J, 20 s) treatment adjunctive to SRP. Ten rats in each group were sacrificed after 7, 15, and 30 days. Histopathological examination was performed in the left mandible of rats. Myeloperoxidase (MPO) was evaluated by western blot in the gingival specimens from the right mandible. Results MPO levels in the laser group were statistically significantly lower compared with the control group (p≤0.05). There was no statistically significance at any time between MPO levels in the control group (p>0.05). MPO levels in the laser group at the 7th day were statistically significantly higher compared to the 15th (p≤0.05) and the 30th day (p≤0.05). Inflammatory cell infiltration decreased over time in both groups and was statistically significantly lower in the laser group than in the control group at all times (p≤0.01). Conclusions Within the limits of this study, we suggest that diode laser application is an adjunctive treatment because it reduced inflammation and MPO when applied in addition to SRP. On the other hand, more studies are needed for the assessment of the effects of diode laser application to periodontal tissues. |
| first_indexed | 2024-12-10T09:42:32Z |
| format | Article |
| id | doaj.art-b29c7fdfc2b94b338f428bf485bb292f |
| institution | Directory Open Access Journal |
| issn | 1678-7765 |
| language | English |
| last_indexed | 2024-12-10T09:42:32Z |
| publishDate | 2018-07-01 |
| publisher | University of São Paulo |
| record_format | Article |
| series | Journal of Applied Oral Science |
| spelling | doaj.art-b29c7fdfc2b94b338f428bf485bb292f2022-12-22T01:53:57ZengUniversity of São PauloJournal of Applied Oral Science1678-77652018-07-0126010.1590/1678-7757-2017-0266S1678-77572018000100483Effects of diode laser application on inflammation and mpo in periodontal tissues in a rat modelMustafa Özay USLUAbubekir ELTASİsmail MARAKOĞLUSerkan DÜNDARKazım ŞAHINİbrahim Hanifi ÖZERCANAbstract Objective In this study, we aimed to histologically and immunologically evaluate the effect of diode laser treatment when applied adjunctive to scaling and root planing (SRP) in an experimental periodontitis model. Materials and methods We used Wistar-Albino rats (n=60) with average weight of 230 g. Experimental periodontitis was induced by ligature at the right and left first mandibular molar teeth in all rats. After 11 days, the ligature was removed and rats were divided into two groups. The control group (n=30) received only SRP treatment, while the laser group (n=30) received a diode laser (GaAlAs, 810 nm, 1 W, 10 J, 20 s) treatment adjunctive to SRP. Ten rats in each group were sacrificed after 7, 15, and 30 days. Histopathological examination was performed in the left mandible of rats. Myeloperoxidase (MPO) was evaluated by western blot in the gingival specimens from the right mandible. Results MPO levels in the laser group were statistically significantly lower compared with the control group (p≤0.05). There was no statistically significance at any time between MPO levels in the control group (p>0.05). MPO levels in the laser group at the 7th day were statistically significantly higher compared to the 15th (p≤0.05) and the 30th day (p≤0.05). Inflammatory cell infiltration decreased over time in both groups and was statistically significantly lower in the laser group than in the control group at all times (p≤0.01). Conclusions Within the limits of this study, we suggest that diode laser application is an adjunctive treatment because it reduced inflammation and MPO when applied in addition to SRP. On the other hand, more studies are needed for the assessment of the effects of diode laser application to periodontal tissues.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-77572018000100483&lng=en&tlng=enExperimental periodontitisInflammation and innate immunityLaserNon-surgical periodontal therapy |
| spellingShingle | Mustafa Özay USLU Abubekir ELTAS İsmail MARAKOĞLU Serkan DÜNDAR Kazım ŞAHIN İbrahim Hanifi ÖZERCAN Effects of diode laser application on inflammation and mpo in periodontal tissues in a rat model Journal of Applied Oral Science Experimental periodontitis Inflammation and innate immunity Laser Non-surgical periodontal therapy |
| title | Effects of diode laser application on inflammation and mpo in periodontal tissues in a rat model |
| title_full | Effects of diode laser application on inflammation and mpo in periodontal tissues in a rat model |
| title_fullStr | Effects of diode laser application on inflammation and mpo in periodontal tissues in a rat model |
| title_full_unstemmed | Effects of diode laser application on inflammation and mpo in periodontal tissues in a rat model |
| title_short | Effects of diode laser application on inflammation and mpo in periodontal tissues in a rat model |
| title_sort | effects of diode laser application on inflammation and mpo in periodontal tissues in a rat model |
| topic | Experimental periodontitis Inflammation and innate immunity Laser Non-surgical periodontal therapy |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-77572018000100483&lng=en&tlng=en |
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