No Association between SARS-CoV-2 Infection and the Polymorphism of the Toll-like Receptor 7 (<i>TLR7</i>) Gene in Female Population
Coronavirus disease 2019 (COVID-19) is caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), a single-stranded RNA virus. Toll-like receptor 7 (<i>TLR7</i>) recognizes single-stranded RNA viruses. The <i>TLR7</i> gene plays a critical role in the human innat...
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| Format: | Article |
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MDPI AG
2023-11-01
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| Series: | Diagnostics |
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| Online Access: | https://www.mdpi.com/2075-4418/13/23/3510 |
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| author | Mohammed Zayed Yong-Chan Kim Chang-Seop Lee Byung-Hoon Jeong |
| author_facet | Mohammed Zayed Yong-Chan Kim Chang-Seop Lee Byung-Hoon Jeong |
| author_sort | Mohammed Zayed |
| collection | DOAJ |
| description | Coronavirus disease 2019 (COVID-19) is caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), a single-stranded RNA virus. Toll-like receptor 7 (<i>TLR7</i>) recognizes single-stranded RNA viruses. The <i>TLR7</i> gene plays a critical role in the human innate and adaptive immune response to SARS-CoV-2 infections. Genetic factors probably affect SARS-CoV-2 infection susceptibility. In the current study, our aim was to search for genetic variations associated with COVID-19 patients in the <i>TLR7</i> gene of a Korean population. We designed five gene-specific primers to cover the coding region of the human <i>TLR7</i> gene. Using amplicon sequencing, we screened the genetic polymorphisms in the coding region of the <i>TLR7</i> gene in COVID-19 patients and healthy controls. The genotype frequencies, allele frequencies, and Hardy–Weinberg equilibrium (HWE) were examined. We identified a low-frequency synonymous single nucleotide polymorphism (SNP) (rs864058) in the coding region of the <i>TLR7</i> gene. There were no significant differences in the genotype or allele frequencies of the <i>TLR7</i> rs864058 polymorphism between COVID-19 female patients and healthy controls (<i>p</i> = 1.0). In conclusion, <i>TLR7</i> (rs864058) polymorphism is low frequency in Korean populations and is not associated with SARS-CoV-2 infection. |
| first_indexed | 2024-03-09T01:54:07Z |
| format | Article |
| id | doaj.art-b29cd6463d464052bbdad271ae5f21f5 |
| institution | Directory Open Access Journal |
| issn | 2075-4418 |
| language | English |
| last_indexed | 2024-03-09T01:54:07Z |
| publishDate | 2023-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Diagnostics |
| spelling | doaj.art-b29cd6463d464052bbdad271ae5f21f52023-12-08T15:13:22ZengMDPI AGDiagnostics2075-44182023-11-011323351010.3390/diagnostics13233510No Association between SARS-CoV-2 Infection and the Polymorphism of the Toll-like Receptor 7 (<i>TLR7</i>) Gene in Female PopulationMohammed Zayed0Yong-Chan Kim1Chang-Seop Lee2Byung-Hoon Jeong3Korea Zoonosis Research Institute, Jeonbuk National University, Iksan 54531, Republic of KoreaDepartment of Biological Sciences, Andong National University, Andong 36729, Republic of KoreaDepartment of Internal Medicine, Research Institute of Clinical Medicine, Jeonbuk National University, Jeonju 54907, Republic of KoreaKorea Zoonosis Research Institute, Jeonbuk National University, Iksan 54531, Republic of KoreaCoronavirus disease 2019 (COVID-19) is caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), a single-stranded RNA virus. Toll-like receptor 7 (<i>TLR7</i>) recognizes single-stranded RNA viruses. The <i>TLR7</i> gene plays a critical role in the human innate and adaptive immune response to SARS-CoV-2 infections. Genetic factors probably affect SARS-CoV-2 infection susceptibility. In the current study, our aim was to search for genetic variations associated with COVID-19 patients in the <i>TLR7</i> gene of a Korean population. We designed five gene-specific primers to cover the coding region of the human <i>TLR7</i> gene. Using amplicon sequencing, we screened the genetic polymorphisms in the coding region of the <i>TLR7</i> gene in COVID-19 patients and healthy controls. The genotype frequencies, allele frequencies, and Hardy–Weinberg equilibrium (HWE) were examined. We identified a low-frequency synonymous single nucleotide polymorphism (SNP) (rs864058) in the coding region of the <i>TLR7</i> gene. There were no significant differences in the genotype or allele frequencies of the <i>TLR7</i> rs864058 polymorphism between COVID-19 female patients and healthy controls (<i>p</i> = 1.0). In conclusion, <i>TLR7</i> (rs864058) polymorphism is low frequency in Korean populations and is not associated with SARS-CoV-2 infection.https://www.mdpi.com/2075-4418/13/23/3510coronavirus disease 2019SARS-CoV-2<i>TLR7</i>genetic variationfemale |
| spellingShingle | Mohammed Zayed Yong-Chan Kim Chang-Seop Lee Byung-Hoon Jeong No Association between SARS-CoV-2 Infection and the Polymorphism of the Toll-like Receptor 7 (<i>TLR7</i>) Gene in Female Population Diagnostics coronavirus disease 2019 SARS-CoV-2 <i>TLR7</i> genetic variation female |
| title | No Association between SARS-CoV-2 Infection and the Polymorphism of the Toll-like Receptor 7 (<i>TLR7</i>) Gene in Female Population |
| title_full | No Association between SARS-CoV-2 Infection and the Polymorphism of the Toll-like Receptor 7 (<i>TLR7</i>) Gene in Female Population |
| title_fullStr | No Association between SARS-CoV-2 Infection and the Polymorphism of the Toll-like Receptor 7 (<i>TLR7</i>) Gene in Female Population |
| title_full_unstemmed | No Association between SARS-CoV-2 Infection and the Polymorphism of the Toll-like Receptor 7 (<i>TLR7</i>) Gene in Female Population |
| title_short | No Association between SARS-CoV-2 Infection and the Polymorphism of the Toll-like Receptor 7 (<i>TLR7</i>) Gene in Female Population |
| title_sort | no association between sars cov 2 infection and the polymorphism of the toll like receptor 7 i tlr7 i gene in female population |
| topic | coronavirus disease 2019 SARS-CoV-2 <i>TLR7</i> genetic variation female |
| url | https://www.mdpi.com/2075-4418/13/23/3510 |
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