Comparability Assessment of Serum Electrolytes on Different Autoanalysers Working on the Same Principle
Introduction: Electrolyte measurement of critically ill patients is frequently requested in emergency central laboratory. These are either obtained from Point of Care (POC) electrolyte analysers on urgent basis or processed on the autoanalysers. Accurate and rapid measurement of electrolyte such...
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| Format: | Article |
| Language: | English |
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JCDR Research and Publications Pvt. Ltd.
2019-04-01
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| Series: | National Journal of Laboratory Medicine |
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| Online Access: | http://www.njlm.net/articles/PDF/2354/40678_CE[Ra1]_F(SHU)_PF1(AG_SHU)_PFA(SHU)_PB(AG_SHU)_PN(SHU).pdf |
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| author | Rashmi Gulia Binita Goswami |
| author_facet | Rashmi Gulia Binita Goswami |
| author_sort | Rashmi Gulia |
| collection | DOAJ |
| description | Introduction: Electrolyte measurement of critically ill patients is
frequently requested in emergency central laboratory. These are
either obtained from Point of Care (POC) electrolyte analysers on
urgent basis or processed on the autoanalysers. Accurate and
rapid measurement of electrolyte such as sodium and potassium
help in development of focused course of treatment.
Aim: To compare the results of serum electrolytes measured on
four different autoanalysers working on the same principle of
direct Ion Selective Electrode (ISE) measurement, to determine
the extent of agreement amongst them.
Materials and Methods: This prospective comparative study
was done using 200 patient samples obtained from the Central
Biochemistry Laboratory of Lok Nayak Hospital, New Delhi,
India. Serum samples were analysed on four different analysers
for electrolytes (Na+ and K+), two Roche AVL electrolyte
analysers and two Randox Imola autoanalysers working on the
same methodology of direct ISE measurement. Means, standard
deviations and coefficient of variations were calculated. The
Bland-Altman plots were used to compare the results of two
different assays and to quantify the limit of agreement. The
p-value <0.05 was considered statistically significant.
Results: The mean value for sodium was 135±10.6 mmol/L and
136±10.4 mmol/L for Roche AVL 1 and 2 electrolyte analysers;
140±10.9 mmol/L and 140±10.7 mmol/L for Randox Imola 1 and
2 autoanalysers. Similarly, the mean value for potassium were
4.53±2.86 mmol/L, 4.32±1.03 mmol/L, 4.33±0.97 mmol/L and
4.35±0.94 mmol/L for AVL 1, AVL 2, Imola 1 and Imola 2, respectively.
The Bland-Altman plots have shown a good agreement of -0.34
to 0.31 for serum potassium for Imola 1 and AVL 1 however, no
such agreement was found for sodium values on inter-analyser
comparison. The Bland-Altman plots were used to compare the
results of two different assays and to quantify the limit of agreement.
The p-value of <0.05 was considered statistically significant.
Conclusion: Serum electrolytes obtained from electrolyte
analysers and autoanalysers showed equivalent results for
potassium but not for sodium values. Therefore, potassium values
can be used interchangeably from these analysers for making
critical decisions but same cannot be concluded for the sodium
values. The comparability will be useful to minimize analytical
bias and allow the results to be used interchangeably. Obtaining
equivalent, rapid and reliable results from ready back-up systems
will prove to be time-saving and economical in emergency cases
irrespective of the analytical system being used. |
| first_indexed | 2024-12-13T06:13:44Z |
| format | Article |
| id | doaj.art-b29e875b60694fbe8b062071c0829447 |
| institution | Directory Open Access Journal |
| issn | 2277-8551 2455-6882 |
| language | English |
| last_indexed | 2024-12-13T06:13:44Z |
| publishDate | 2019-04-01 |
| publisher | JCDR Research and Publications Pvt. Ltd. |
| record_format | Article |
| series | National Journal of Laboratory Medicine |
| spelling | doaj.art-b29e875b60694fbe8b062071c08294472022-12-21T23:57:02ZengJCDR Research and Publications Pvt. Ltd.National Journal of Laboratory Medicine2277-85512455-68822019-04-0182BO12BO1510.7860/NJLM/2019/40678:2354Comparability Assessment of Serum Electrolytes on Different Autoanalysers Working on the Same PrincipleRashmi Gulia0Binita Goswami1Senior Resident, Department of Biochemistry, MAMC, Delhi, India.Professor, Department of Biochemistry, MAMC, New Delhi, India.Introduction: Electrolyte measurement of critically ill patients is frequently requested in emergency central laboratory. These are either obtained from Point of Care (POC) electrolyte analysers on urgent basis or processed on the autoanalysers. Accurate and rapid measurement of electrolyte such as sodium and potassium help in development of focused course of treatment. Aim: To compare the results of serum electrolytes measured on four different autoanalysers working on the same principle of direct Ion Selective Electrode (ISE) measurement, to determine the extent of agreement amongst them. Materials and Methods: This prospective comparative study was done using 200 patient samples obtained from the Central Biochemistry Laboratory of Lok Nayak Hospital, New Delhi, India. Serum samples were analysed on four different analysers for electrolytes (Na+ and K+), two Roche AVL electrolyte analysers and two Randox Imola autoanalysers working on the same methodology of direct ISE measurement. Means, standard deviations and coefficient of variations were calculated. The Bland-Altman plots were used to compare the results of two different assays and to quantify the limit of agreement. The p-value <0.05 was considered statistically significant. Results: The mean value for sodium was 135±10.6 mmol/L and 136±10.4 mmol/L for Roche AVL 1 and 2 electrolyte analysers; 140±10.9 mmol/L and 140±10.7 mmol/L for Randox Imola 1 and 2 autoanalysers. Similarly, the mean value for potassium were 4.53±2.86 mmol/L, 4.32±1.03 mmol/L, 4.33±0.97 mmol/L and 4.35±0.94 mmol/L for AVL 1, AVL 2, Imola 1 and Imola 2, respectively. The Bland-Altman plots have shown a good agreement of -0.34 to 0.31 for serum potassium for Imola 1 and AVL 1 however, no such agreement was found for sodium values on inter-analyser comparison. The Bland-Altman plots were used to compare the results of two different assays and to quantify the limit of agreement. The p-value of <0.05 was considered statistically significant. Conclusion: Serum electrolytes obtained from electrolyte analysers and autoanalysers showed equivalent results for potassium but not for sodium values. Therefore, potassium values can be used interchangeably from these analysers for making critical decisions but same cannot be concluded for the sodium values. The comparability will be useful to minimize analytical bias and allow the results to be used interchangeably. Obtaining equivalent, rapid and reliable results from ready back-up systems will prove to be time-saving and economical in emergency cases irrespective of the analytical system being used.http://www.njlm.net/articles/PDF/2354/40678_CE[Ra1]_F(SHU)_PF1(AG_SHU)_PFA(SHU)_PB(AG_SHU)_PN(SHU).pdfagreementbland and altmanion selective electrodepotassiumsodium |
| spellingShingle | Rashmi Gulia Binita Goswami Comparability Assessment of Serum Electrolytes on Different Autoanalysers Working on the Same Principle National Journal of Laboratory Medicine agreement bland and altman ion selective electrode potassium sodium |
| title | Comparability Assessment of Serum Electrolytes on Different Autoanalysers Working on the Same Principle |
| title_full | Comparability Assessment of Serum Electrolytes on Different Autoanalysers Working on the Same Principle |
| title_fullStr | Comparability Assessment of Serum Electrolytes on Different Autoanalysers Working on the Same Principle |
| title_full_unstemmed | Comparability Assessment of Serum Electrolytes on Different Autoanalysers Working on the Same Principle |
| title_short | Comparability Assessment of Serum Electrolytes on Different Autoanalysers Working on the Same Principle |
| title_sort | comparability assessment of serum electrolytes on different autoanalysers working on the same principle |
| topic | agreement bland and altman ion selective electrode potassium sodium |
| url | http://www.njlm.net/articles/PDF/2354/40678_CE[Ra1]_F(SHU)_PF1(AG_SHU)_PFA(SHU)_PB(AG_SHU)_PN(SHU).pdf |
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