Protocol for analyzing γδ T cells from the vagina of diet-induced obese mice using single-cell RNA sequencing and flow cytometry

Protocol for analyzing γδ T cells from the vagina of diet-induced obese mice using single-cell RNA sequencing and flow cytometry

Summary: Obesity affects susceptibility to sexually transmitted diseases like genital herpes, caused by herpes simplex virus (HSV) 2. The γδ T cells in the vagina play a major role in HSV-2 suppression. Here, we present a protocol for inducing HSV-2 infection intravaginally in high-fat diet-induced...

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Bibliographic Details
Main Authors: In Kang, Jang Hyun Park, Yumin Kim, Myoung Seung Kwon, Heung Kyu Lee
Format: Article
Language:English
Published: Elsevier 2023-06-01
Series:STAR Protocols
Subjects:
Sequence analysis
Cell culture
Cell isolation
Single Cell
Flow Cytometry/Mass Cytometry
Cell separation/fractionation
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166723002459

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