The cagE gene sequence as a diagnostic marker to identify JP2 and non-JP2 highly leukotoxic Aggregatibacter actinomycetemcomitans serotype b strains
Aggregatibacter actinomycetemcomitans is associated with aggressive forms of periodontitis, and with systemic infections such as endocarditis. The cagE gene encodes a ≈39-kDa putative exotoxin expressed by A. actinomycetemcomitans. The prevalence of cagE, and its significance in periodontal disease,...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2017-05-01
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| Series: | Journal of Oral Microbiology |
| Online Access: | http://dx.doi.org/10.1080/20002297.2017.1325257 |
| _version_ | 1818447836386164736 |
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| author | Anders Johansson Rolf Claesson Carola Höglund Åberg Dorte Haubek Jan Oscarsson |
| author_facet | Anders Johansson Rolf Claesson Carola Höglund Åberg Dorte Haubek Jan Oscarsson |
| author_sort | Anders Johansson |
| collection | DOAJ |
| description | Aggregatibacter actinomycetemcomitans is associated with aggressive forms of periodontitis, and with systemic infections such as endocarditis. The cagE gene encodes a ≈39-kDa putative exotoxin expressed by A. actinomycetemcomitans. The prevalence of cagE, and its significance in periodontal disease, has not yet been thoroughly evaluated. In the present study, the role of the cagE gene as a diagnostic marker has been investigated. We have used PCR, and whole genome sequencing data to determine the prevalence of cagE in A. actinomycetemcomitans based on analysis of (i) 249 isolates, collected and cultivated in a Ghanaian longitudinal cohort study, (ii) a serotype b collection of 19 strains, and (iii) the 36 A. actinomycetemcomitans genomes available in the NCBI database. Whereas cagE was absent in the other serotypes, this gene sequence was linked to a virulent and highly leukotoxic group of serotype b strains, including both JP2 and non-JP2 genotypes. We therefore propose that cagE has a potential to be used as a PCR-based gene marker for the identification of a virulent and highly leukotoxic group of serotype b strains, including both JP2 and non-JP2 genotypes. This might be of importance in the risk assessment of the development of periodontal attachment loss in young individuals. |
| first_indexed | 2024-12-14T20:09:57Z |
| format | Article |
| id | doaj.art-b2a35e1f6aba4f75be3ff84046be20d5 |
| institution | Directory Open Access Journal |
| issn | 2000-2297 |
| language | English |
| last_indexed | 2024-12-14T20:09:57Z |
| publishDate | 2017-05-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Journal of Oral Microbiology |
| spelling | doaj.art-b2a35e1f6aba4f75be3ff84046be20d52022-12-21T22:48:57ZengTaylor & Francis GroupJournal of Oral Microbiology2000-22972017-05-019010.1080/20002297.2017.13252571325257The cagE gene sequence as a diagnostic marker to identify JP2 and non-JP2 highly leukotoxic Aggregatibacter actinomycetemcomitans serotype b strainsAnders Johansson0Rolf Claesson1Carola Höglund Åberg2Dorte Haubek3Jan Oscarsson4Umeå UniversityUmeå UniversityUmeå UniversityOral Health Aarhus UniversityUmeå UniversityAggregatibacter actinomycetemcomitans is associated with aggressive forms of periodontitis, and with systemic infections such as endocarditis. The cagE gene encodes a ≈39-kDa putative exotoxin expressed by A. actinomycetemcomitans. The prevalence of cagE, and its significance in periodontal disease, has not yet been thoroughly evaluated. In the present study, the role of the cagE gene as a diagnostic marker has been investigated. We have used PCR, and whole genome sequencing data to determine the prevalence of cagE in A. actinomycetemcomitans based on analysis of (i) 249 isolates, collected and cultivated in a Ghanaian longitudinal cohort study, (ii) a serotype b collection of 19 strains, and (iii) the 36 A. actinomycetemcomitans genomes available in the NCBI database. Whereas cagE was absent in the other serotypes, this gene sequence was linked to a virulent and highly leukotoxic group of serotype b strains, including both JP2 and non-JP2 genotypes. We therefore propose that cagE has a potential to be used as a PCR-based gene marker for the identification of a virulent and highly leukotoxic group of serotype b strains, including both JP2 and non-JP2 genotypes. This might be of importance in the risk assessment of the development of periodontal attachment loss in young individuals.http://dx.doi.org/10.1080/20002297.2017.1325257 |
| spellingShingle | Anders Johansson Rolf Claesson Carola Höglund Åberg Dorte Haubek Jan Oscarsson The cagE gene sequence as a diagnostic marker to identify JP2 and non-JP2 highly leukotoxic Aggregatibacter actinomycetemcomitans serotype b strains Journal of Oral Microbiology |
| title | The cagE gene sequence as a diagnostic marker to identify JP2 and non-JP2 highly leukotoxic Aggregatibacter actinomycetemcomitans serotype b strains |
| title_full | The cagE gene sequence as a diagnostic marker to identify JP2 and non-JP2 highly leukotoxic Aggregatibacter actinomycetemcomitans serotype b strains |
| title_fullStr | The cagE gene sequence as a diagnostic marker to identify JP2 and non-JP2 highly leukotoxic Aggregatibacter actinomycetemcomitans serotype b strains |
| title_full_unstemmed | The cagE gene sequence as a diagnostic marker to identify JP2 and non-JP2 highly leukotoxic Aggregatibacter actinomycetemcomitans serotype b strains |
| title_short | The cagE gene sequence as a diagnostic marker to identify JP2 and non-JP2 highly leukotoxic Aggregatibacter actinomycetemcomitans serotype b strains |
| title_sort | cage gene sequence as a diagnostic marker to identify jp2 and non jp2 highly leukotoxic aggregatibacter actinomycetemcomitans serotype b strains |
| url | http://dx.doi.org/10.1080/20002297.2017.1325257 |
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