Production of Recombinant Biopharmaceuticals in <i>Chlamydomonas reinhardtii</i>
This review aimed to present <i>Chlamydomonas reinhardtii</i> as an alternative for heterologous protein production, especially for biopharmaceuticals, and its general characteristics when compared with other expression systems. The need to produce heterologous proteins for industrial in...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-12-01
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| Series: | International Journal of Plant Biology |
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| Online Access: | https://www.mdpi.com/2037-0164/14/1/4 |
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| author | Cesar Andres Diaz Arias Caroline Frere Martiniuc de Oliveira João Vitor Dutra Molino Livia Seno Ferreira-Camargo Marcelo Chuei Matsudo João Carlos Monteiro de Carvalho |
| author_facet | Cesar Andres Diaz Arias Caroline Frere Martiniuc de Oliveira João Vitor Dutra Molino Livia Seno Ferreira-Camargo Marcelo Chuei Matsudo João Carlos Monteiro de Carvalho |
| author_sort | Cesar Andres Diaz Arias |
| collection | DOAJ |
| description | This review aimed to present <i>Chlamydomonas reinhardtii</i> as an alternative for heterologous protein production, especially for biopharmaceuticals, and its general characteristics when compared with other expression systems. The need to produce heterologous proteins for industrial interest, therapeutic ends, and diagnostic kits has led to the development of recombinant microalgal technology. This technology presents some interesting features, such as rapid growth and low transgene dispersion compared to plants, the ability to fold complex proteins compared to bacteria, and low production costs compared to other expression systems, such as yeast and mammalian cells. Overall, <i>C. reinhardtii</i> heterologous protein expression is coming of age with several research groups focused on developing an optimal producer strain. |
| first_indexed | 2024-03-11T06:24:20Z |
| format | Article |
| id | doaj.art-b2a5bef211794dc6aeea4e37507f79d9 |
| institution | Directory Open Access Journal |
| issn | 2037-0164 |
| language | English |
| last_indexed | 2024-03-11T06:24:20Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Plant Biology |
| spelling | doaj.art-b2a5bef211794dc6aeea4e37507f79d92023-11-17T11:42:33ZengMDPI AGInternational Journal of Plant Biology2037-01642022-12-01141395210.3390/ijpb14010004Production of Recombinant Biopharmaceuticals in <i>Chlamydomonas reinhardtii</i>Cesar Andres Diaz Arias0Caroline Frere Martiniuc de Oliveira1João Vitor Dutra Molino2Livia Seno Ferreira-Camargo3Marcelo Chuei Matsudo4João Carlos Monteiro de Carvalho5Department of Biochemical and Pharmaceutical Technology, Faculty of Pharmaceutical Sciences, University of São Paulo, Av. Prof. Lineu Prestes 580, Bl. 16, São Paulo 05508-900, SP, BrazilCenter of Natural and Human Sciences, Federal University of ABC, Av. dos Estados, 5001, Santo André 09210-580, SP, BrazilDepartment of Biochemical and Pharmaceutical Technology, Faculty of Pharmaceutical Sciences, University of São Paulo, Av. Prof. Lineu Prestes 580, Bl. 16, São Paulo 05508-900, SP, BrazilCenter of Natural and Human Sciences, Federal University of ABC, Av. dos Estados, 5001, Santo André 09210-580, SP, BrazilCenter of Natural and Human Sciences, Federal University of ABC, Av. dos Estados, 5001, Santo André 09210-580, SP, BrazilDepartment of Biochemical and Pharmaceutical Technology, Faculty of Pharmaceutical Sciences, University of São Paulo, Av. Prof. Lineu Prestes 580, Bl. 16, São Paulo 05508-900, SP, BrazilThis review aimed to present <i>Chlamydomonas reinhardtii</i> as an alternative for heterologous protein production, especially for biopharmaceuticals, and its general characteristics when compared with other expression systems. The need to produce heterologous proteins for industrial interest, therapeutic ends, and diagnostic kits has led to the development of recombinant microalgal technology. This technology presents some interesting features, such as rapid growth and low transgene dispersion compared to plants, the ability to fold complex proteins compared to bacteria, and low production costs compared to other expression systems, such as yeast and mammalian cells. Overall, <i>C. reinhardtii</i> heterologous protein expression is coming of age with several research groups focused on developing an optimal producer strain.https://www.mdpi.com/2037-0164/14/1/4<i>Chlamydomonas reinhardtii</i>heterologous proteinsmicroalgaeexpression system |
| spellingShingle | Cesar Andres Diaz Arias Caroline Frere Martiniuc de Oliveira João Vitor Dutra Molino Livia Seno Ferreira-Camargo Marcelo Chuei Matsudo João Carlos Monteiro de Carvalho Production of Recombinant Biopharmaceuticals in <i>Chlamydomonas reinhardtii</i> International Journal of Plant Biology <i>Chlamydomonas reinhardtii</i> heterologous proteins microalgae expression system |
| title | Production of Recombinant Biopharmaceuticals in <i>Chlamydomonas reinhardtii</i> |
| title_full | Production of Recombinant Biopharmaceuticals in <i>Chlamydomonas reinhardtii</i> |
| title_fullStr | Production of Recombinant Biopharmaceuticals in <i>Chlamydomonas reinhardtii</i> |
| title_full_unstemmed | Production of Recombinant Biopharmaceuticals in <i>Chlamydomonas reinhardtii</i> |
| title_short | Production of Recombinant Biopharmaceuticals in <i>Chlamydomonas reinhardtii</i> |
| title_sort | production of recombinant biopharmaceuticals in i chlamydomonas reinhardtii i |
| topic | <i>Chlamydomonas reinhardtii</i> heterologous proteins microalgae expression system |
| url | https://www.mdpi.com/2037-0164/14/1/4 |
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