Toxic Effects of Arsenic in Commercially Important Fish Rohu Carp, <i>Labeo rohita</i> of Bangladesh
This study was designed to elucidate the effects of arsenic (As) on the morpho-behavior, growth development and molecular mechanisms of a commercially important fish, rohu carp, <i>Labeo rohita</i>, in Bangladesh. Fish fry with an average weight of 387.5 ± 169.25 mg and an average length...
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MDPI AG
2022-08-01
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| Цуврал: | Fishes |
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| Онлайн хандалт: | https://www.mdpi.com/2410-3888/7/5/217 |
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| author | Md. Golam Rabbane Md. Alamgir Kabir Md. Habibullah-Al-Mamun Md. Ghulam Mustafa |
| author_facet | Md. Golam Rabbane Md. Alamgir Kabir Md. Habibullah-Al-Mamun Md. Ghulam Mustafa |
| author_sort | Md. Golam Rabbane |
| collection | DOAJ |
| description | This study was designed to elucidate the effects of arsenic (As) on the morpho-behavior, growth development and molecular mechanisms of a commercially important fish, rohu carp, <i>Labeo rohita</i>, in Bangladesh. Fish fry with an average weight of 387.5 ± 169.25 mg and an average length of 3.35 ± 0.37 cm were collected from a local hatchery in Mymensingh, Bangladesh and acclimatized for a week in the Department of Fisheries, University of Dhaka before starting the exposure with arsenic. Fishes were exposed for a period of 14 days with three treatments of NaAsO<sub>2</sub>, namely treatment 1(T1)—2.5 mg/L; treatment 2 (T2)—15 mg/L; and treatment 3 (T3)—30 mg/L, along with a control (C)—0.0 mg/L, with three replicates. These concentrations were determined based on the LC<sub>50</sub> value for 96 h measured for this experiment. This study revealed remarkable morphological abnormalities and deformities in arsenic-exposed rohu carp. In fish exposed to 30 mg/L, caudal fin erosion was a frequent deformity. There was no significant difference in RNA:DNA ratio among the treatments. The overall weight of fish was decreased as the concentration of arsenic was increased. The T3 fish had a statistically significant negative weight gain (−0.05 ± 0.07 g), but the other treatments (T1 and T2) and control fish had no significant weight gain. Different types of histopathological changes were observed in the gills and intestines of arsenic-treated fish. Necrosis and severe damages were found in the secondary lamellae of gills at the highest arsenic concentration (30 mg/L). Epithelial lifting, irregular shape and damages in the gill raker were also observed in the primary lamellae of the gills for the same treatment. In this study, the expression of <i>heat shock protein</i> (<i>HSP 60</i>) and <i>metallothionein</i> (<i>MT</i>) genes was assessed by qPCR, and these genes were upregulated in different treatments compared to controls. The findings of the present study suggest that arsenic pollution significantly changes the morphology, behavior, growth, development, histopathology and molecular mechanisms of this economically important fish, rohu carp, in Bangladesh. |
| first_indexed | 2024-03-09T20:13:52Z |
| format | Article |
| id | doaj.art-b2ae5fdbfa214d90876733d0d95c6a9c |
| institution | Directory Open Access Journal |
| issn | 2410-3888 |
| language | English |
| last_indexed | 2024-03-09T20:13:52Z |
| publishDate | 2022-08-01 |
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| series | Fishes |
| spelling | doaj.art-b2ae5fdbfa214d90876733d0d95c6a9c2023-11-24T00:05:42ZengMDPI AGFishes2410-38882022-08-017521710.3390/fishes7050217Toxic Effects of Arsenic in Commercially Important Fish Rohu Carp, <i>Labeo rohita</i> of BangladeshMd. Golam Rabbane0Md. Alamgir Kabir1Md. Habibullah-Al-Mamun2Md. Ghulam Mustafa3Department of Fisheries, Faculty of Biological Sciences, University of Dhaka, Dhaka 1000, BangladeshDepartment of Fisheries, Faculty of Biological Sciences, University of Dhaka, Dhaka 1000, BangladeshDepartment of Fisheries, Faculty of Biological Sciences, University of Dhaka, Dhaka 1000, BangladeshDepartment of Fisheries, Faculty of Biological Sciences, University of Dhaka, Dhaka 1000, BangladeshThis study was designed to elucidate the effects of arsenic (As) on the morpho-behavior, growth development and molecular mechanisms of a commercially important fish, rohu carp, <i>Labeo rohita</i>, in Bangladesh. Fish fry with an average weight of 387.5 ± 169.25 mg and an average length of 3.35 ± 0.37 cm were collected from a local hatchery in Mymensingh, Bangladesh and acclimatized for a week in the Department of Fisheries, University of Dhaka before starting the exposure with arsenic. Fishes were exposed for a period of 14 days with three treatments of NaAsO<sub>2</sub>, namely treatment 1(T1)—2.5 mg/L; treatment 2 (T2)—15 mg/L; and treatment 3 (T3)—30 mg/L, along with a control (C)—0.0 mg/L, with three replicates. These concentrations were determined based on the LC<sub>50</sub> value for 96 h measured for this experiment. This study revealed remarkable morphological abnormalities and deformities in arsenic-exposed rohu carp. In fish exposed to 30 mg/L, caudal fin erosion was a frequent deformity. There was no significant difference in RNA:DNA ratio among the treatments. The overall weight of fish was decreased as the concentration of arsenic was increased. The T3 fish had a statistically significant negative weight gain (−0.05 ± 0.07 g), but the other treatments (T1 and T2) and control fish had no significant weight gain. Different types of histopathological changes were observed in the gills and intestines of arsenic-treated fish. Necrosis and severe damages were found in the secondary lamellae of gills at the highest arsenic concentration (30 mg/L). Epithelial lifting, irregular shape and damages in the gill raker were also observed in the primary lamellae of the gills for the same treatment. In this study, the expression of <i>heat shock protein</i> (<i>HSP 60</i>) and <i>metallothionein</i> (<i>MT</i>) genes was assessed by qPCR, and these genes were upregulated in different treatments compared to controls. The findings of the present study suggest that arsenic pollution significantly changes the morphology, behavior, growth, development, histopathology and molecular mechanisms of this economically important fish, rohu carp, in Bangladesh.https://www.mdpi.com/2410-3888/7/5/217arsenicrohu carphistopathologygrowthRNA:DNA ratiogene expression |
| spellingShingle | Md. Golam Rabbane Md. Alamgir Kabir Md. Habibullah-Al-Mamun Md. Ghulam Mustafa Toxic Effects of Arsenic in Commercially Important Fish Rohu Carp, <i>Labeo rohita</i> of Bangladesh Fishes arsenic rohu carp histopathology growth RNA:DNA ratio gene expression |
| title | Toxic Effects of Arsenic in Commercially Important Fish Rohu Carp, <i>Labeo rohita</i> of Bangladesh |
| title_full | Toxic Effects of Arsenic in Commercially Important Fish Rohu Carp, <i>Labeo rohita</i> of Bangladesh |
| title_fullStr | Toxic Effects of Arsenic in Commercially Important Fish Rohu Carp, <i>Labeo rohita</i> of Bangladesh |
| title_full_unstemmed | Toxic Effects of Arsenic in Commercially Important Fish Rohu Carp, <i>Labeo rohita</i> of Bangladesh |
| title_short | Toxic Effects of Arsenic in Commercially Important Fish Rohu Carp, <i>Labeo rohita</i> of Bangladesh |
| title_sort | toxic effects of arsenic in commercially important fish rohu carp i labeo rohita i of bangladesh |
| topic | arsenic rohu carp histopathology growth RNA:DNA ratio gene expression |
| url | https://www.mdpi.com/2410-3888/7/5/217 |
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