IL-18 PROMOTER POLYMORPHISM AND IL-18 SERUM LEVELS IN ASSOCIATION WITH RELAPSING-REMITTING MULTIPLE SCLEROSIS
Purpose: Multiple sclerosis is the immune-mediated disorder whose etiology is not completely understood. The present study aimed to survey association between the promoter polymorphism IL18 -607C/A (rs1946518), the serum concentration of IL-18 and susceptibility to relapsing-remitting multiple scler...
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| Format: | Article |
| Language: | English |
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Peytchinski Publishing
2020-12-01
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| Series: | Journal of IMAB |
| Subjects: | |
| Online Access: | https://www.journal-imab-bg.org/issues-2020/issue4/2020vol26-issue4-3475-3480.pdf |
| _version_ | 1818594853877972992 |
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| author | Boncho Grigorov Anastasiya Trenova Antonia Grigorova Spaska Stanilova |
| author_facet | Boncho Grigorov Anastasiya Trenova Antonia Grigorova Spaska Stanilova |
| author_sort | Boncho Grigorov |
| collection | DOAJ |
| description | Purpose: Multiple sclerosis is the immune-mediated disorder whose etiology is not completely understood. The present study aimed to survey association between the promoter polymorphism IL18 -607C/A (rs1946518), the serum concentration of IL-18 and susceptibility to relapsing-remitting multiple sclerosis (RRMS) in Bulgarian patients
Material and Methods: This case-control study includes 159 RRMS patients with disease-modifying therapy (DMT) and 162 age-sex-matched healthy volunteers. All included subjects were genotyped by ARMS-PCR while serum levels were measured by enzyme-linked immunosorbent assay (ELISA).
Results: Our results revealed significant differences in the serum levels of IL-18 according to the gender, the onset of disease and the type of disease-modifying therapy. The serum levels of IL-18 are significantly higher in RRMS men compared to RRMS women and the RRMS men with late-onset of the disease (above 30 years) also demonstrated significantly increased serum levels than the women with late-onset of disease and even with healthy men. The RRMS patients treated with interferon-beta showed significantly increased IL-18 serum level than those treated with glatiramer acetate.
Conclusions: Our study shows that the promoter polymorphism IL18 -607C/A is not associated with susceptibility to RRMS in Bulgarian patients as well as the serum level of the cytokine. The observed differences in the serum level of IL-18 in RRMS patients according to gender and the response to therapy could be used as a biomarker to the course of the disease. |
| first_indexed | 2024-12-16T11:06:44Z |
| format | Article |
| id | doaj.art-b2b15448bcf94be6907d733b03f9a67c |
| institution | Directory Open Access Journal |
| issn | 1312-773X |
| language | English |
| last_indexed | 2024-12-16T11:06:44Z |
| publishDate | 2020-12-01 |
| publisher | Peytchinski Publishing |
| record_format | Article |
| series | Journal of IMAB |
| spelling | doaj.art-b2b15448bcf94be6907d733b03f9a67c2022-12-21T22:33:50ZengPeytchinski PublishingJournal of IMAB1312-773X2020-12-012643475348010.5272/jimab.2020264.3475IL-18 PROMOTER POLYMORPHISM AND IL-18 SERUM LEVELS IN ASSOCIATION WITH RELAPSING-REMITTING MULTIPLE SCLEROSISBoncho Grigorov0https://orcid.org/0000-0001-6446-9316Anastasiya Trenova1https://orcid.org/0000-0002-1847-4752Antonia Grigorova2https://orcid.org/0000-0002-6459-8182Spaska Stanilova3https://orcid.org/0000-0003-1368-9081Department of Molecular Biology, Immunology and Medical Genetics, Medical Faculty Trakia University, Stara Zagora, BulgariaDepartment of Neurology, Faculty of Medicine, Medical University of Plovdiv, Bulgaria.Department of Molecular Biology, Immunology and Medical Genetics, Medical Faculty Trakia University, Stara Zagora, BulgariaDepartment of Molecular Biology, Immunology and Medical Genetics, Medical Faculty Trakia University, Stara Zagora, BulgariaPurpose: Multiple sclerosis is the immune-mediated disorder whose etiology is not completely understood. The present study aimed to survey association between the promoter polymorphism IL18 -607C/A (rs1946518), the serum concentration of IL-18 and susceptibility to relapsing-remitting multiple sclerosis (RRMS) in Bulgarian patients Material and Methods: This case-control study includes 159 RRMS patients with disease-modifying therapy (DMT) and 162 age-sex-matched healthy volunteers. All included subjects were genotyped by ARMS-PCR while serum levels were measured by enzyme-linked immunosorbent assay (ELISA). Results: Our results revealed significant differences in the serum levels of IL-18 according to the gender, the onset of disease and the type of disease-modifying therapy. The serum levels of IL-18 are significantly higher in RRMS men compared to RRMS women and the RRMS men with late-onset of the disease (above 30 years) also demonstrated significantly increased serum levels than the women with late-onset of disease and even with healthy men. The RRMS patients treated with interferon-beta showed significantly increased IL-18 serum level than those treated with glatiramer acetate. Conclusions: Our study shows that the promoter polymorphism IL18 -607C/A is not associated with susceptibility to RRMS in Bulgarian patients as well as the serum level of the cytokine. The observed differences in the serum level of IL-18 in RRMS patients according to gender and the response to therapy could be used as a biomarker to the course of the disease.https://www.journal-imab-bg.org/issues-2020/issue4/2020vol26-issue4-3475-3480.pdfrelapsing-remitting multiple sclerosiscytokiners1946518snp |
| spellingShingle | Boncho Grigorov Anastasiya Trenova Antonia Grigorova Spaska Stanilova IL-18 PROMOTER POLYMORPHISM AND IL-18 SERUM LEVELS IN ASSOCIATION WITH RELAPSING-REMITTING MULTIPLE SCLEROSIS Journal of IMAB relapsing-remitting multiple sclerosis cytokine rs1946518 snp |
| title | IL-18 PROMOTER POLYMORPHISM AND IL-18 SERUM LEVELS IN ASSOCIATION WITH RELAPSING-REMITTING MULTIPLE SCLEROSIS |
| title_full | IL-18 PROMOTER POLYMORPHISM AND IL-18 SERUM LEVELS IN ASSOCIATION WITH RELAPSING-REMITTING MULTIPLE SCLEROSIS |
| title_fullStr | IL-18 PROMOTER POLYMORPHISM AND IL-18 SERUM LEVELS IN ASSOCIATION WITH RELAPSING-REMITTING MULTIPLE SCLEROSIS |
| title_full_unstemmed | IL-18 PROMOTER POLYMORPHISM AND IL-18 SERUM LEVELS IN ASSOCIATION WITH RELAPSING-REMITTING MULTIPLE SCLEROSIS |
| title_short | IL-18 PROMOTER POLYMORPHISM AND IL-18 SERUM LEVELS IN ASSOCIATION WITH RELAPSING-REMITTING MULTIPLE SCLEROSIS |
| title_sort | il 18 promoter polymorphism and il 18 serum levels in association with relapsing remitting multiple sclerosis |
| topic | relapsing-remitting multiple sclerosis cytokine rs1946518 snp |
| url | https://www.journal-imab-bg.org/issues-2020/issue4/2020vol26-issue4-3475-3480.pdf |
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