Detection and Characterization of Feline Calicivirus Associated with Paw and Mouth Disease
Feline calicivirus (FCV) infection in cats can led to several diverse clinical presentations, ranging from mild upper respiratory signs to virulent systemic disease. Herein, we report a paw and mouth disease case in a 7-year-old household cat due to an FCV infection. An asymptomatic cat living in th...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-12-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/13/1/65 |
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| author | Andrea Palombieri Vittorio Sarchese Maria Veronica Giordano Paola Fruci Paolo Emidio Crisi Giovanni Aste Laura Bongiovanni Valentina Rinaldi Alessio Sposato Michele Camero Gianvito Lanave Vito Martella Fulvio Marsilio Barbara Di Martino Federica Di Profio |
| author_facet | Andrea Palombieri Vittorio Sarchese Maria Veronica Giordano Paola Fruci Paolo Emidio Crisi Giovanni Aste Laura Bongiovanni Valentina Rinaldi Alessio Sposato Michele Camero Gianvito Lanave Vito Martella Fulvio Marsilio Barbara Di Martino Federica Di Profio |
| author_sort | Andrea Palombieri |
| collection | DOAJ |
| description | Feline calicivirus (FCV) infection in cats can led to several diverse clinical presentations, ranging from mild upper respiratory signs to virulent systemic disease. Herein, we report a paw and mouth disease case in a 7-year-old household cat due to an FCV infection. An asymptomatic cat living in the same household was also infected with FCV. Clinical and pathological investigations were combined with the molecular and phenotypical characterization of the FCV strains. The RNA of the FCV was detected using qualitative and quantitative reverse transcription (RT)-PCR assays, and FCV antigen was confirmed by immunohistochemistry. After the whole genome analysis, the strains detected in the two cats appeared to be genetically diverse from FCVs previously detected in association with paw and mouth disease and with virulent systemic disease. Interestingly, the isolates obtained in this study were resistant to low pH conditions and slightly susceptible to bile salts, but they were susceptible to a trypsin treatment, revealing a phenotype pattern that is different from that which has been observed for respiratory FCVs. |
| first_indexed | 2024-03-11T10:13:06Z |
| format | Article |
| id | doaj.art-b2b15ab56f354ae086e1eb273feda75d |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-11T10:13:06Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-b2b15ab56f354ae086e1eb273feda75d2023-11-16T14:18:38ZengMDPI AGAnimals2076-26152022-12-011316510.3390/ani13010065Detection and Characterization of Feline Calicivirus Associated with Paw and Mouth DiseaseAndrea Palombieri0Vittorio Sarchese1Maria Veronica Giordano2Paola Fruci3Paolo Emidio Crisi4Giovanni Aste5Laura Bongiovanni6Valentina Rinaldi7Alessio Sposato8Michele Camero9Gianvito Lanave10Vito Martella11Fulvio Marsilio12Barbara Di Martino13Federica Di Profio14Department of Veterinary Medicine, Università degli Studi di Teramo, 64100 Teramo, ItalyDepartment of Veterinary Medicine, Università degli Studi di Teramo, 64100 Teramo, ItalyDepartment of Veterinary Medicine, Università degli Studi di Teramo, 64100 Teramo, ItalyDepartment of Veterinary Medicine, Università degli Studi di Teramo, 64100 Teramo, ItalyDepartment of Veterinary Medicine, Università degli Studi di Teramo, 64100 Teramo, ItalyDepartment of Veterinary Medicine, Università degli Studi di Teramo, 64100 Teramo, ItalyDepartment of Veterinary Medicine, Università degli Studi di Teramo, 64100 Teramo, ItalyDepartment of Veterinary Medicine, Università degli Studi di Teramo, 64100 Teramo, ItalyDepartment of Veterinary Medicine, Università Aldo Moro di Bari, 70121 Bari, ItalyDepartment of Veterinary Medicine, Università Aldo Moro di Bari, 70121 Bari, ItalyDepartment of Veterinary Medicine, Università Aldo Moro di Bari, 70121 Bari, ItalyDepartment of Veterinary Medicine, Università Aldo Moro di Bari, 70121 Bari, ItalyDepartment of Veterinary Medicine, Università degli Studi di Teramo, 64100 Teramo, ItalyDepartment of Veterinary Medicine, Università degli Studi di Teramo, 64100 Teramo, ItalyDepartment of Veterinary Medicine, Università degli Studi di Teramo, 64100 Teramo, ItalyFeline calicivirus (FCV) infection in cats can led to several diverse clinical presentations, ranging from mild upper respiratory signs to virulent systemic disease. Herein, we report a paw and mouth disease case in a 7-year-old household cat due to an FCV infection. An asymptomatic cat living in the same household was also infected with FCV. Clinical and pathological investigations were combined with the molecular and phenotypical characterization of the FCV strains. The RNA of the FCV was detected using qualitative and quantitative reverse transcription (RT)-PCR assays, and FCV antigen was confirmed by immunohistochemistry. After the whole genome analysis, the strains detected in the two cats appeared to be genetically diverse from FCVs previously detected in association with paw and mouth disease and with virulent systemic disease. Interestingly, the isolates obtained in this study were resistant to low pH conditions and slightly susceptible to bile salts, but they were susceptible to a trypsin treatment, revealing a phenotype pattern that is different from that which has been observed for respiratory FCVs.https://www.mdpi.com/2076-2615/13/1/65FCVpaw and mouth diseaseimmunohistochemistryWGSphenotypic and antigenic characterization |
| spellingShingle | Andrea Palombieri Vittorio Sarchese Maria Veronica Giordano Paola Fruci Paolo Emidio Crisi Giovanni Aste Laura Bongiovanni Valentina Rinaldi Alessio Sposato Michele Camero Gianvito Lanave Vito Martella Fulvio Marsilio Barbara Di Martino Federica Di Profio Detection and Characterization of Feline Calicivirus Associated with Paw and Mouth Disease Animals FCV paw and mouth disease immunohistochemistry WGS phenotypic and antigenic characterization |
| title | Detection and Characterization of Feline Calicivirus Associated with Paw and Mouth Disease |
| title_full | Detection and Characterization of Feline Calicivirus Associated with Paw and Mouth Disease |
| title_fullStr | Detection and Characterization of Feline Calicivirus Associated with Paw and Mouth Disease |
| title_full_unstemmed | Detection and Characterization of Feline Calicivirus Associated with Paw and Mouth Disease |
| title_short | Detection and Characterization of Feline Calicivirus Associated with Paw and Mouth Disease |
| title_sort | detection and characterization of feline calicivirus associated with paw and mouth disease |
| topic | FCV paw and mouth disease immunohistochemistry WGS phenotypic and antigenic characterization |
| url | https://www.mdpi.com/2076-2615/13/1/65 |
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