Protocol to decellularize porcine right ventricular outflow tracts using a 3D printed flow chamber
Summary: Surgical treatment of pediatric congenital heart disease with tissue grafts is a lifesaving intervention. Decellularization to reduce immunogenicity of tissue grafts is an increasingly popular alternative to glutaraldehyde fixation. Here, we present a protocol to decellularize porcine right...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2024-03-01
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| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166724000649 |
| _version_ | 1827348243653066752 |
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| author | Amy G. Harris Vico Schot Michele Carrabba Dominga Iacobazzi Mohamed T. Ghorbel James P.K. Armstrong Adam W. Perriman Massimo Caputo Giovanni Biglino Francesca Bartoli-Leonard |
| author_facet | Amy G. Harris Vico Schot Michele Carrabba Dominga Iacobazzi Mohamed T. Ghorbel James P.K. Armstrong Adam W. Perriman Massimo Caputo Giovanni Biglino Francesca Bartoli-Leonard |
| author_sort | Amy G. Harris |
| collection | DOAJ |
| description | Summary: Surgical treatment of pediatric congenital heart disease with tissue grafts is a lifesaving intervention. Decellularization to reduce immunogenicity of tissue grafts is an increasingly popular alternative to glutaraldehyde fixation. Here, we present a protocol to decellularize porcine right ventricular outflow tracts using a 3D printed flow chamber. We describe steps for 3D printing the flow rig, preparing porcine tissue, and using the flow rig to utilize shear forces for decellularization. We then detail procedures for characterizing the acellular scaffold.For complete details on the use and execution of this protocol, please refer to Vafaee et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| first_indexed | 2024-03-08T00:09:26Z |
| format | Article |
| id | doaj.art-b2b186d6fd6c4b6daab76d30fb44b9da |
| institution | Directory Open Access Journal |
| issn | 2666-1667 |
| language | English |
| last_indexed | 2024-03-08T00:09:26Z |
| publishDate | 2024-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj.art-b2b186d6fd6c4b6daab76d30fb44b9da2024-02-17T06:42:18ZengElsevierSTAR Protocols2666-16672024-03-0151102899Protocol to decellularize porcine right ventricular outflow tracts using a 3D printed flow chamberAmy G. Harris0Vico Schot1Michele Carrabba2Dominga Iacobazzi3Mohamed T. Ghorbel4James P.K. Armstrong5Adam W. Perriman6Massimo Caputo7Giovanni Biglino8Francesca Bartoli-Leonard9Bristol Heart Institute, Bristol Medical School, University of Bristol, BS2 8HW Bristol, UK; Department of Translational Health Sciences, Bristol Medical School, University of Bristol, BS1 3NY Bristol, UK; Corresponding authorBristol Heart Institute, Bristol Medical School, University of Bristol, BS2 8HW Bristol, UK; Department of Translational Health Sciences, Bristol Medical School, University of Bristol, BS1 3NY Bristol, UKBristol Heart Institute, Bristol Medical School, University of Bristol, BS2 8HW Bristol, UK; Department of Translational Health Sciences, Bristol Medical School, University of Bristol, BS1 3NY Bristol, UKBristol Heart Institute, Bristol Medical School, University of Bristol, BS2 8HW Bristol, UK; Department of Translational Health Sciences, Bristol Medical School, University of Bristol, BS1 3NY Bristol, UKBristol Heart Institute, Bristol Medical School, University of Bristol, BS2 8HW Bristol, UK; Department of Translational Health Sciences, Bristol Medical School, University of Bristol, BS1 3NY Bristol, UKDepartment of Translational Health Sciences, Bristol Medical School, University of Bristol, BS1 3NY Bristol, UKSchool of Cellular and Molecular Medicine, Biomedical Sciences Building, University Walk, BS8 1TD Bristol, UKBristol Heart Institute, Bristol Medical School, University of Bristol, BS2 8HW Bristol, UK; Department of Translational Health Sciences, Bristol Medical School, University of Bristol, BS1 3NY Bristol, UK; Cardiac Surgery, University Hospitals Bristol, NHS Foundation Trust, BS2 8HW Bristol, UKBristol Heart Institute, Bristol Medical School, University of Bristol, BS2 8HW Bristol, UK; Department of Translational Health Sciences, Bristol Medical School, University of Bristol, BS1 3NY Bristol, UK; Cardiorespiratory Unit, Great Ormond Street Hospital for Children, NHS Foundation Trust, WC1N 3JH London, UKBristol Heart Institute, Bristol Medical School, University of Bristol, BS2 8HW Bristol, UK; Department of Translational Health Sciences, Bristol Medical School, University of Bristol, BS1 3NY Bristol, UK; Corresponding authorSummary: Surgical treatment of pediatric congenital heart disease with tissue grafts is a lifesaving intervention. Decellularization to reduce immunogenicity of tissue grafts is an increasingly popular alternative to glutaraldehyde fixation. Here, we present a protocol to decellularize porcine right ventricular outflow tracts using a 3D printed flow chamber. We describe steps for 3D printing the flow rig, preparing porcine tissue, and using the flow rig to utilize shear forces for decellularization. We then detail procedures for characterizing the acellular scaffold.For complete details on the use and execution of this protocol, please refer to Vafaee et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.http://www.sciencedirect.com/science/article/pii/S2666166724000649ImmunologyTissue Engineering |
| spellingShingle | Amy G. Harris Vico Schot Michele Carrabba Dominga Iacobazzi Mohamed T. Ghorbel James P.K. Armstrong Adam W. Perriman Massimo Caputo Giovanni Biglino Francesca Bartoli-Leonard Protocol to decellularize porcine right ventricular outflow tracts using a 3D printed flow chamber STAR Protocols Immunology Tissue Engineering |
| title | Protocol to decellularize porcine right ventricular outflow tracts using a 3D printed flow chamber |
| title_full | Protocol to decellularize porcine right ventricular outflow tracts using a 3D printed flow chamber |
| title_fullStr | Protocol to decellularize porcine right ventricular outflow tracts using a 3D printed flow chamber |
| title_full_unstemmed | Protocol to decellularize porcine right ventricular outflow tracts using a 3D printed flow chamber |
| title_short | Protocol to decellularize porcine right ventricular outflow tracts using a 3D printed flow chamber |
| title_sort | protocol to decellularize porcine right ventricular outflow tracts using a 3d printed flow chamber |
| topic | Immunology Tissue Engineering |
| url | http://www.sciencedirect.com/science/article/pii/S2666166724000649 |
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