Children with autism show differences in the gut DNA virome compared to non-autistic children: a case control study
Abstract Background Several previous studies have identified a potential role that the gut microbiome can play in autism spectrum disorder (ASD) in children, but little is known about how variations in the virome may be involved in ASD. We aimed to understand the changes in the gut DNA virome of chi...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2023-04-01
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| Series: | BMC Pediatrics |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12887-023-03981-8 |
| _version_ | 1797845764673634304 |
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| author | Aina Qu Boyang Duan Yue Wang Zhenzhen Cui Nuochen Zhang De Wu |
| author_facet | Aina Qu Boyang Duan Yue Wang Zhenzhen Cui Nuochen Zhang De Wu |
| author_sort | Aina Qu |
| collection | DOAJ |
| description | Abstract Background Several previous studies have identified a potential role that the gut microbiome can play in autism spectrum disorder (ASD) in children, but little is known about how variations in the virome may be involved in ASD. We aimed to understand the changes in the gut DNA virome of children with ASD. Methods A case–control study was presented, in which 13 two-children families were observed while considering the age, mode of birth, history of antibiotic use, and vaccination history to minimize the influence of confounding factors. DNA viral metagenomic sequencing was successfully performed on stool samples from 11 children with ASD and 12 healthy non-ASD children. The basic composition and gene function of the participants' fecal DNA virome were detected and analyzed. Finally, the abundance and diversity of the DNA virome of children with ASD and their healthy siblings were compared. Results The gut DNA virome in children aged 3–11 years was found to be dominated by the Siphoviridae family of Caudovirales. The proteins encoded by the DNA genes mainly carry out the functions of genetic information transmission and metabolism. Compared the gut DNA virome of ASD and healthy non-ASD children, their abundance of Caudovirales and Petitvirales both showed a significant negative correlation (r = -0.902, P < 0.01), there was no statistically significant difference in the relative abundance of viruses at the order and family levels, and a difference in the relative abundance at the genus level for Skunavirus (Ζ = -2.157, P = 0.031). Viral α diversity was reduced in children with ASD, but α diversity and β diversity did not differ statistically between groups. Conclusions This study indicates that elevated Skunavirus abundance and decreased α diversity in the gut DNA virulence group of children with ASD, but no statistically significant difference in the change in alpha and beta diversity. This provides preliminary cumulative information on virological aspects of the relationship between the microbiome and ASD, and should benefit future multi-omics and large sample studies on the gut microbes in children with ASD. |
| first_indexed | 2024-04-09T17:44:10Z |
| format | Article |
| id | doaj.art-b2b187c28ac542749a468a8297175c07 |
| institution | Directory Open Access Journal |
| issn | 1471-2431 |
| language | English |
| last_indexed | 2024-04-09T17:44:10Z |
| publishDate | 2023-04-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Pediatrics |
| spelling | doaj.art-b2b187c28ac542749a468a8297175c072023-04-16T11:25:25ZengBMCBMC Pediatrics1471-24312023-04-0123111510.1186/s12887-023-03981-8Children with autism show differences in the gut DNA virome compared to non-autistic children: a case control studyAina Qu0Boyang Duan1Yue Wang2Zhenzhen Cui3Nuochen Zhang4De Wu5Pediatric Neurological Rehabilitation Center, The First Affiliated Hospital of Anhui Medical UniversityPediatric Neurological Rehabilitation Center, The First Affiliated Hospital of Anhui Medical UniversityPediatric Neurological Rehabilitation Center, The First Affiliated Hospital of Anhui Medical UniversityPediatric Neurological Rehabilitation Center, The First Affiliated Hospital of Anhui Medical UniversityPediatric Neurological Rehabilitation Center, The First Affiliated Hospital of Anhui Medical UniversityPediatric Neurological Rehabilitation Center, The First Affiliated Hospital of Anhui Medical UniversityAbstract Background Several previous studies have identified a potential role that the gut microbiome can play in autism spectrum disorder (ASD) in children, but little is known about how variations in the virome may be involved in ASD. We aimed to understand the changes in the gut DNA virome of children with ASD. Methods A case–control study was presented, in which 13 two-children families were observed while considering the age, mode of birth, history of antibiotic use, and vaccination history to minimize the influence of confounding factors. DNA viral metagenomic sequencing was successfully performed on stool samples from 11 children with ASD and 12 healthy non-ASD children. The basic composition and gene function of the participants' fecal DNA virome were detected and analyzed. Finally, the abundance and diversity of the DNA virome of children with ASD and their healthy siblings were compared. Results The gut DNA virome in children aged 3–11 years was found to be dominated by the Siphoviridae family of Caudovirales. The proteins encoded by the DNA genes mainly carry out the functions of genetic information transmission and metabolism. Compared the gut DNA virome of ASD and healthy non-ASD children, their abundance of Caudovirales and Petitvirales both showed a significant negative correlation (r = -0.902, P < 0.01), there was no statistically significant difference in the relative abundance of viruses at the order and family levels, and a difference in the relative abundance at the genus level for Skunavirus (Ζ = -2.157, P = 0.031). Viral α diversity was reduced in children with ASD, but α diversity and β diversity did not differ statistically between groups. Conclusions This study indicates that elevated Skunavirus abundance and decreased α diversity in the gut DNA virulence group of children with ASD, but no statistically significant difference in the change in alpha and beta diversity. This provides preliminary cumulative information on virological aspects of the relationship between the microbiome and ASD, and should benefit future multi-omics and large sample studies on the gut microbes in children with ASD.https://doi.org/10.1186/s12887-023-03981-8Autism spectrum disorderGut microbiomeGut viromeMetagenomeSkunavirus |
| spellingShingle | Aina Qu Boyang Duan Yue Wang Zhenzhen Cui Nuochen Zhang De Wu Children with autism show differences in the gut DNA virome compared to non-autistic children: a case control study BMC Pediatrics Autism spectrum disorder Gut microbiome Gut virome Metagenome Skunavirus |
| title | Children with autism show differences in the gut DNA virome compared to non-autistic children: a case control study |
| title_full | Children with autism show differences in the gut DNA virome compared to non-autistic children: a case control study |
| title_fullStr | Children with autism show differences in the gut DNA virome compared to non-autistic children: a case control study |
| title_full_unstemmed | Children with autism show differences in the gut DNA virome compared to non-autistic children: a case control study |
| title_short | Children with autism show differences in the gut DNA virome compared to non-autistic children: a case control study |
| title_sort | children with autism show differences in the gut dna virome compared to non autistic children a case control study |
| topic | Autism spectrum disorder Gut microbiome Gut virome Metagenome Skunavirus |
| url | https://doi.org/10.1186/s12887-023-03981-8 |
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