Transcriptomic patterns in early-secretory and mid-secretory endometrium in a natural menstrual cycle immediately before fertilization and embryo transfer
Objective This study aimed to evaluate the endometrial transcriptomic patterns in the early secretory phase (ESP) and mid-secretory phase (MSP) of the natural menstrual cycle before in vitro fertilization and embryo transfer (IVF-ET). Methods Thirty patients whose endometrial tissues were obtained f...
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| Format: | Article |
| Language: | English |
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Korean Society of Obstetrics and Gynecology
2023-09-01
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| Series: | Obstetrics & Gynecology Science |
| Subjects: | |
| Online Access: | http://ogscience.org/upload/pdf/ogs-22315.pdf |
| _version_ | 1827813801093758976 |
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| author | Sung Pil Choo Inha Lee Jae-Hoon Lee Dowon Lee Hyemin Park Joo Hyun Park SiHyun Cho Young Sik Choi |
| author_facet | Sung Pil Choo Inha Lee Jae-Hoon Lee Dowon Lee Hyemin Park Joo Hyun Park SiHyun Cho Young Sik Choi |
| author_sort | Sung Pil Choo |
| collection | DOAJ |
| description | Objective This study aimed to evaluate the endometrial transcriptomic patterns in the early secretory phase (ESP) and mid-secretory phase (MSP) of the natural menstrual cycle before in vitro fertilization and embryo transfer (IVF-ET). Methods Thirty patients whose endometrial tissues were obtained from the ESP or MSP of a natural menstrual cycle immediately before IVF-ET were included. Endometrial dating was histologically confirmed as ESP (cycle days 16–18) or MSP (cycle days 19–21), according to the noyes criteria. The patients were divided into two groups depending on the IVF-ET outcome: pregnant (n=14; 7 in ESP and 7 in MSP) or non-pregnant (n=16; 8 in ESP and 8 in MSP). Differentially expressed genes (DEGs) in the MSP, compared to the ESP, were identified using NanoString nCounter (NanoString Technologies, Seattle, WA, USA) data for both the pregnant and non-pregnant groups. Results Thirteen DEGs in the pregnant group and 11 DEGs in the non-pregnant group were identified in the MSP compared to those in the ESP. In both groups, adrenoceptor alpha 2A, interleukin 1 receptor-associated kinase 2, a disintegrin and metalloproteinase with thrombospondin repeats 15 (ADAMTS15), serpin family E member 1, integrin subunit beta 3, transmembrane protein 252 (TMEM252), huntingtin associated protein 1, C2 calcium-dependent domain containing 4A, and integrin subunit alpha 2 were upregulated in the MSP, compared to the ESP. TMEM37, galactosidase beta 1 like 2, Rho family GTPase 3, and cytochrome P450 family 24 subfamily A member 1 were upregulated in the MSP only in the pregnant group. ADAMTS8 was downregulated and monoamine oxidase A was upregulated in the MSP only in the non-pregnant group. Conclusion Transcriptomic patterns in the endometrium immediately before IVF-ET appear to differ according to the IVF-ET outcome. These novel DEGs, which have not been previously studied, may have functional significance during the window of implantation and serve as potential biomarkers of endometrial receptivity. |
| first_indexed | 2024-03-11T23:36:13Z |
| format | Article |
| id | doaj.art-b2b19c14631041d4b00149700068d015 |
| institution | Directory Open Access Journal |
| issn | 2287-8572 2287-8580 |
| language | English |
| last_indexed | 2024-03-11T23:36:13Z |
| publishDate | 2023-09-01 |
| publisher | Korean Society of Obstetrics and Gynecology |
| record_format | Article |
| series | Obstetrics & Gynecology Science |
| spelling | doaj.art-b2b19c14631041d4b00149700068d0152023-09-19T23:08:10ZengKorean Society of Obstetrics and GynecologyObstetrics & Gynecology Science2287-85722287-85802023-09-0166541742910.5468/ogs.223158766Transcriptomic patterns in early-secretory and mid-secretory endometrium in a natural menstrual cycle immediately before fertilization and embryo transferSung Pil Choo0Inha Lee1Jae-Hoon Lee2Dowon Lee3Hyemin Park4Joo Hyun Park5SiHyun Cho6Young Sik Choi7 Department of Obstetrics and Gynecology, Inha University Hospital, College of Medicine, Inha University, Incheon, Korea Department of Obstetrics and Gynecology, Gangnam Severance Hospital, Yonsei University College of Medicine, Seoul, Korea Department of Obstetrics and Gynecology, Gangnam Severance Hospital, Yonsei University College of Medicine, Seoul, Korea Department of Obstetrics and Gynecology, Severance Hospital, Yonsei University College of Medicine, Seoul, Korea Department of Obstetrics and Gynecology, Severance Hospital, Yonsei University College of Medicine, Seoul, Korea Department of Obstetrics and Gynecology, Institute of Women’s Life Medical Science, Yonsei University College of Medicine, Seoul, Korea Department of Obstetrics and Gynecology, Gangnam Severance Hospital, Yonsei University College of Medicine, Seoul, Korea Department of Obstetrics and Gynecology, Institute of Women’s Life Medical Science, Yonsei University College of Medicine, Seoul, KoreaObjective This study aimed to evaluate the endometrial transcriptomic patterns in the early secretory phase (ESP) and mid-secretory phase (MSP) of the natural menstrual cycle before in vitro fertilization and embryo transfer (IVF-ET). Methods Thirty patients whose endometrial tissues were obtained from the ESP or MSP of a natural menstrual cycle immediately before IVF-ET were included. Endometrial dating was histologically confirmed as ESP (cycle days 16–18) or MSP (cycle days 19–21), according to the noyes criteria. The patients were divided into two groups depending on the IVF-ET outcome: pregnant (n=14; 7 in ESP and 7 in MSP) or non-pregnant (n=16; 8 in ESP and 8 in MSP). Differentially expressed genes (DEGs) in the MSP, compared to the ESP, were identified using NanoString nCounter (NanoString Technologies, Seattle, WA, USA) data for both the pregnant and non-pregnant groups. Results Thirteen DEGs in the pregnant group and 11 DEGs in the non-pregnant group were identified in the MSP compared to those in the ESP. In both groups, adrenoceptor alpha 2A, interleukin 1 receptor-associated kinase 2, a disintegrin and metalloproteinase with thrombospondin repeats 15 (ADAMTS15), serpin family E member 1, integrin subunit beta 3, transmembrane protein 252 (TMEM252), huntingtin associated protein 1, C2 calcium-dependent domain containing 4A, and integrin subunit alpha 2 were upregulated in the MSP, compared to the ESP. TMEM37, galactosidase beta 1 like 2, Rho family GTPase 3, and cytochrome P450 family 24 subfamily A member 1 were upregulated in the MSP only in the pregnant group. ADAMTS8 was downregulated and monoamine oxidase A was upregulated in the MSP only in the non-pregnant group. Conclusion Transcriptomic patterns in the endometrium immediately before IVF-ET appear to differ according to the IVF-ET outcome. These novel DEGs, which have not been previously studied, may have functional significance during the window of implantation and serve as potential biomarkers of endometrial receptivity.http://ogscience.org/upload/pdf/ogs-22315.pdfendometriumluteal phasetranscriptomeembryo implantation |
| spellingShingle | Sung Pil Choo Inha Lee Jae-Hoon Lee Dowon Lee Hyemin Park Joo Hyun Park SiHyun Cho Young Sik Choi Transcriptomic patterns in early-secretory and mid-secretory endometrium in a natural menstrual cycle immediately before fertilization and embryo transfer Obstetrics & Gynecology Science endometrium luteal phase transcriptome embryo implantation |
| title | Transcriptomic patterns in early-secretory and mid-secretory endometrium in a natural menstrual cycle immediately before fertilization and embryo transfer |
| title_full | Transcriptomic patterns in early-secretory and mid-secretory endometrium in a natural menstrual cycle immediately before fertilization and embryo transfer |
| title_fullStr | Transcriptomic patterns in early-secretory and mid-secretory endometrium in a natural menstrual cycle immediately before fertilization and embryo transfer |
| title_full_unstemmed | Transcriptomic patterns in early-secretory and mid-secretory endometrium in a natural menstrual cycle immediately before fertilization and embryo transfer |
| title_short | Transcriptomic patterns in early-secretory and mid-secretory endometrium in a natural menstrual cycle immediately before fertilization and embryo transfer |
| title_sort | transcriptomic patterns in early secretory and mid secretory endometrium in a natural menstrual cycle immediately before fertilization and embryo transfer |
| topic | endometrium luteal phase transcriptome embryo implantation |
| url | http://ogscience.org/upload/pdf/ogs-22315.pdf |
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