Let it glow: genetically encoded fluorescent reporters in Plasmodium
Abstract The use of fluorescent proteins (FPs) in Plasmodium parasites has been key to understand the biology of this obligate intracellular protozoon. FPs like the green fluorescent protein (GFP) enabled to explore protein localization, promoter activity as well as dynamic processes like protein ex...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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BMC
2024-04-01
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| Series: | Malaria Journal |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12936-024-04936-9 |
| _version_ | 1797199628903383040 |
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| author | Pia J. Thiele Raquel Mela-Lopez Stéphanie A. Blandin Dennis Klug |
| author_facet | Pia J. Thiele Raquel Mela-Lopez Stéphanie A. Blandin Dennis Klug |
| author_sort | Pia J. Thiele |
| collection | DOAJ |
| description | Abstract The use of fluorescent proteins (FPs) in Plasmodium parasites has been key to understand the biology of this obligate intracellular protozoon. FPs like the green fluorescent protein (GFP) enabled to explore protein localization, promoter activity as well as dynamic processes like protein export and endocytosis. Furthermore, FP biosensors have provided detailed information on physiological parameters at the subcellular level, and fluorescent reporter lines greatly extended the malariology toolbox. Still, in order to achieve optimal results, it is crucial to know exactly the properties of the FP of choice and the genetic scenario in which it will be used. This review highlights advantages and disadvantages of available landing sites and promoters that have been successfully applied for the ectopic expression of FPs in Plasmodium berghei and Plasmodium falciparum. Furthermore, the properties of newly developed FPs beyond DsRed and EGFP, in the visualization of cells and cellular structures as well as in the sensing of small molecules are discussed. Graphical Abstract |
| first_indexed | 2024-04-24T07:18:47Z |
| format | Article |
| id | doaj.art-b2b359d88d3342089b6ac1f234c2793f |
| institution | Directory Open Access Journal |
| issn | 1475-2875 |
| language | English |
| last_indexed | 2024-04-24T07:18:47Z |
| publishDate | 2024-04-01 |
| publisher | BMC |
| record_format | Article |
| series | Malaria Journal |
| spelling | doaj.art-b2b359d88d3342089b6ac1f234c2793f2024-04-21T11:09:03ZengBMCMalaria Journal1475-28752024-04-0123111510.1186/s12936-024-04936-9Let it glow: genetically encoded fluorescent reporters in PlasmodiumPia J. Thiele0Raquel Mela-Lopez1Stéphanie A. Blandin2Dennis Klug3Inserm, CNRS, Université de Strasbourg, UPR9022/U1257, Mosquito Immune Responses (MIR), IBMCInserm, CNRS, Université de Strasbourg, UPR9022/U1257, Mosquito Immune Responses (MIR), IBMCInserm, CNRS, Université de Strasbourg, UPR9022/U1257, Mosquito Immune Responses (MIR), IBMCInserm, CNRS, Université de Strasbourg, UPR9022/U1257, Mosquito Immune Responses (MIR), IBMCAbstract The use of fluorescent proteins (FPs) in Plasmodium parasites has been key to understand the biology of this obligate intracellular protozoon. FPs like the green fluorescent protein (GFP) enabled to explore protein localization, promoter activity as well as dynamic processes like protein export and endocytosis. Furthermore, FP biosensors have provided detailed information on physiological parameters at the subcellular level, and fluorescent reporter lines greatly extended the malariology toolbox. Still, in order to achieve optimal results, it is crucial to know exactly the properties of the FP of choice and the genetic scenario in which it will be used. This review highlights advantages and disadvantages of available landing sites and promoters that have been successfully applied for the ectopic expression of FPs in Plasmodium berghei and Plasmodium falciparum. Furthermore, the properties of newly developed FPs beyond DsRed and EGFP, in the visualization of cells and cellular structures as well as in the sensing of small molecules are discussed. Graphical Abstracthttps://doi.org/10.1186/s12936-024-04936-9MalariaPlasmodiumFluorescent proteinGFPApicomplexaParasite |
| spellingShingle | Pia J. Thiele Raquel Mela-Lopez Stéphanie A. Blandin Dennis Klug Let it glow: genetically encoded fluorescent reporters in Plasmodium Malaria Journal Malaria Plasmodium Fluorescent protein GFP Apicomplexa Parasite |
| title | Let it glow: genetically encoded fluorescent reporters in Plasmodium |
| title_full | Let it glow: genetically encoded fluorescent reporters in Plasmodium |
| title_fullStr | Let it glow: genetically encoded fluorescent reporters in Plasmodium |
| title_full_unstemmed | Let it glow: genetically encoded fluorescent reporters in Plasmodium |
| title_short | Let it glow: genetically encoded fluorescent reporters in Plasmodium |
| title_sort | let it glow genetically encoded fluorescent reporters in plasmodium |
| topic | Malaria Plasmodium Fluorescent protein GFP Apicomplexa Parasite |
| url | https://doi.org/10.1186/s12936-024-04936-9 |
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