High-Resolution Hyperspectral Microscopic Imaging With Single Acousto-Optic Tunable Filter Based on Double Filtering
Hyperspectral imaging is a technique that integrates multiple spectral bands and image information. Its applications range from improving the accuracy of cancer diagnosis to testing the quality of products. Here, we introduce a double-filtering technique that provides high-resolution diagnostic hist...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
IEEE
2020-01-01
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| Series: | IEEE Access |
| Subjects: | |
| Online Access: | https://ieeexplore.ieee.org/document/8946633/ |
| _version_ | 1818642915501539328 |
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| author | Xiaofa Zhang Chunguang Zhang Yupuyun Wang Hao Wang Zhenfei Sheng Zhiwei Tan Weijie Qiu Xi Huang Pengchong Wang Wenyao Liu Haiping Tong Yuhao Liu Xiansheng Wang |
| author_facet | Xiaofa Zhang Chunguang Zhang Yupuyun Wang Hao Wang Zhenfei Sheng Zhiwei Tan Weijie Qiu Xi Huang Pengchong Wang Wenyao Liu Haiping Tong Yuhao Liu Xiansheng Wang |
| author_sort | Xiaofa Zhang |
| collection | DOAJ |
| description | Hyperspectral imaging is a technique that integrates multiple spectral bands and image information. Its applications range from improving the accuracy of cancer diagnosis to testing the quality of products. Here, we introduce a double-filtering technique that provides high-resolution diagnostic histological images within minutes. The hyperspectral microscopic imaging system is built based on an acousto-optic tunable filter (AOTF). The optimized system is analyzed from the perspective of spectrum and imaging. The spectral resolution can be improved by 37.08 % to 59.95% in the visible light range. The side lobe is obviously inhibited and the purity of spectrum is improved. Furthermore, the example of hyperspectral microscopic imaging is demonstrated with unstained gastric cancer tissue sections to assess the ability of the system in terms of its spectral performances and image quality. The microscopic imaging results of single filtering optical path system and single crystal double filtering optical path system are compared. In general, the optimized double filtering system achieves excellent performance in bandwidth compression and side lobe suppression, especially the first application of hyperspectral microscopy combined with microscope in the visible light range. |
| first_indexed | 2024-12-16T23:50:39Z |
| format | Article |
| id | doaj.art-b2b5f628c315442982f857bf81c7ad78 |
| institution | Directory Open Access Journal |
| issn | 2169-3536 |
| language | English |
| last_indexed | 2024-12-16T23:50:39Z |
| publishDate | 2020-01-01 |
| publisher | IEEE |
| record_format | Article |
| series | IEEE Access |
| spelling | doaj.art-b2b5f628c315442982f857bf81c7ad782022-12-21T22:11:21ZengIEEEIEEE Access2169-35362020-01-018115701157610.1109/ACCESS.2019.29633698946633High-Resolution Hyperspectral Microscopic Imaging With Single Acousto-Optic Tunable Filter Based on Double FilteringXiaofa Zhang0https://orcid.org/0000-0002-0909-6905Chunguang Zhang1https://orcid.org/0000-0002-5747-7593Yupuyun Wang2https://orcid.org/0000-0002-4871-3371Hao Wang3https://orcid.org/0000-0003-0637-5762Zhenfei Sheng4https://orcid.org/0000-0003-4688-3606Zhiwei Tan5https://orcid.org/0000-0002-0533-3272Weijie Qiu6https://orcid.org/0000-0001-6517-3058Xi Huang7https://orcid.org/0000-0001-7054-153XPengchong Wang8https://orcid.org/0000-0001-5664-0355Wenyao Liu9https://orcid.org/0000-0002-1763-8954Haiping Tong10https://orcid.org/0000-0001-7633-4523Yuhao Liu11https://orcid.org/0000-0003-3700-4876Xiansheng Wang12https://orcid.org/0000-0001-7214-0908Key Laboratory of Optoelectronic Science and Technology for Medicine, Fujian Provincial Key Laboratory of Photonics Technology, Fujian Provincial Engineering Technology Research Center of Photoelectric Sensing Application, College of Photonic and Electronic Engineering, Ministry of Education, Fujian Normal University, Fujian, ChinaKey Laboratory of Optoelectronic Science and Technology for Medicine, Fujian Provincial Key Laboratory of Photonics Technology, Fujian Provincial Engineering Technology Research Center of Photoelectric Sensing Application, College of Photonic and Electronic Engineering, Ministry of Education, Fujian Normal University, Fujian, ChinaKey Laboratory of Optoelectronic Science and Technology for Medicine, Fujian Provincial Key Laboratory of Photonics Technology, Fujian Provincial Engineering Technology Research Center of Photoelectric Sensing Application, College of Photonic and Electronic Engineering, Ministry of Education, Fujian Normal University, Fujian, ChinaKey Laboratory of Optoelectronic Science and Technology for Medicine, Fujian Provincial Key Laboratory of Photonics Technology, Fujian Provincial Engineering Technology Research Center of Photoelectric Sensing Application, College of Photonic and Electronic Engineering, Ministry of Education, Fujian Normal University, Fujian, ChinaKey Laboratory of Optoelectronic Science and Technology for Medicine, Fujian Provincial Key Laboratory of Photonics Technology, Fujian Provincial Engineering Technology Research Center of Photoelectric Sensing Application, College of Photonic and Electronic Engineering, Ministry of Education, Fujian Normal University, Fujian, ChinaKey Laboratory of Optoelectronic Science and Technology for Medicine, Fujian Provincial Key Laboratory of Photonics Technology, Fujian Provincial Engineering Technology Research Center of Photoelectric Sensing Application, College of Photonic and Electronic Engineering, Ministry of Education, Fujian Normal University, Fujian, ChinaKey Laboratory of Optoelectronic Science and Technology for Medicine, Fujian Provincial Key Laboratory of Photonics Technology, Fujian Provincial Engineering Technology Research Center of Photoelectric Sensing Application, College of Photonic and Electronic Engineering, Ministry of Education, Fujian Normal University, Fujian, ChinaKey Laboratory of Optoelectronic Science and Technology for Medicine, Fujian Provincial Key Laboratory of Photonics Technology, Fujian Provincial Engineering Technology Research Center of Photoelectric Sensing Application, College of Photonic and Electronic Engineering, Ministry of Education, Fujian Normal University, Fujian, ChinaKey Laboratory of Optoelectronic Science and Technology for Medicine, Fujian Provincial Key Laboratory of Photonics Technology, Fujian Provincial Engineering Technology Research Center of Photoelectric Sensing Application, College of Photonic and Electronic Engineering, Ministry of Education, Fujian Normal University, Fujian, ChinaFujian Normal University Hospital, Fuzhou, ChinaKey Laboratory of Optoelectronic Science and Technology for Medicine, Fujian Provincial Key Laboratory of Photonics Technology, Fujian Provincial Engineering Technology Research Center of Photoelectric Sensing Application, College of Photonic and Electronic Engineering, Ministry of Education, Fujian Normal University, Fujian, ChinaKey Laboratory of Optoelectronic Science and Technology for Medicine, Fujian Provincial Key Laboratory of Photonics Technology, Fujian Provincial Engineering Technology Research Center of Photoelectric Sensing Application, College of Photonic and Electronic Engineering, Ministry of Education, Fujian Normal University, Fujian, ChinaKey Laboratory of Optoelectronic Science and Technology for Medicine, Fujian Provincial Key Laboratory of Photonics Technology, Fujian Provincial Engineering Technology Research Center of Photoelectric Sensing Application, College of Photonic and Electronic Engineering, Ministry of Education, Fujian Normal University, Fujian, ChinaHyperspectral imaging is a technique that integrates multiple spectral bands and image information. Its applications range from improving the accuracy of cancer diagnosis to testing the quality of products. Here, we introduce a double-filtering technique that provides high-resolution diagnostic histological images within minutes. The hyperspectral microscopic imaging system is built based on an acousto-optic tunable filter (AOTF). The optimized system is analyzed from the perspective of spectrum and imaging. The spectral resolution can be improved by 37.08 % to 59.95% in the visible light range. The side lobe is obviously inhibited and the purity of spectrum is improved. Furthermore, the example of hyperspectral microscopic imaging is demonstrated with unstained gastric cancer tissue sections to assess the ability of the system in terms of its spectral performances and image quality. The microscopic imaging results of single filtering optical path system and single crystal double filtering optical path system are compared. In general, the optimized double filtering system achieves excellent performance in bandwidth compression and side lobe suppression, especially the first application of hyperspectral microscopy combined with microscope in the visible light range.https://ieeexplore.ieee.org/document/8946633/Acousto-optical devicebirefringencehigh-resolution imagingoptical device fabrication |
| spellingShingle | Xiaofa Zhang Chunguang Zhang Yupuyun Wang Hao Wang Zhenfei Sheng Zhiwei Tan Weijie Qiu Xi Huang Pengchong Wang Wenyao Liu Haiping Tong Yuhao Liu Xiansheng Wang High-Resolution Hyperspectral Microscopic Imaging With Single Acousto-Optic Tunable Filter Based on Double Filtering IEEE Access Acousto-optical device birefringence high-resolution imaging optical device fabrication |
| title | High-Resolution Hyperspectral Microscopic Imaging With Single Acousto-Optic Tunable Filter Based on Double Filtering |
| title_full | High-Resolution Hyperspectral Microscopic Imaging With Single Acousto-Optic Tunable Filter Based on Double Filtering |
| title_fullStr | High-Resolution Hyperspectral Microscopic Imaging With Single Acousto-Optic Tunable Filter Based on Double Filtering |
| title_full_unstemmed | High-Resolution Hyperspectral Microscopic Imaging With Single Acousto-Optic Tunable Filter Based on Double Filtering |
| title_short | High-Resolution Hyperspectral Microscopic Imaging With Single Acousto-Optic Tunable Filter Based on Double Filtering |
| title_sort | high resolution hyperspectral microscopic imaging with single acousto optic tunable filter based on double filtering |
| topic | Acousto-optical device birefringence high-resolution imaging optical device fabrication |
| url | https://ieeexplore.ieee.org/document/8946633/ |
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