Spatial analysis of the metastatic brain tumor immune and extracellular matrix microenvironment
Metastatic cancer is responsible for the overwhelming majority of cancer-related deaths, with metastatic tumors being the most common neoplasms affecting the central nervous system. One of the major factors regulating tumor biology is the tumor microenvironment. However, little is known about the ce...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-07-01
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| Series: | Advances in Cancer Biology - Metastasis |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2667394023000102 |
| _version_ | 1797799917584908288 |
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| author | Samuel S. Widodo Marija Dinevska Lucero Cuzcano Michael Papanicolaou Thomas R. Cox Stanley S. Stylli Theo Mantamadiotis |
| author_facet | Samuel S. Widodo Marija Dinevska Lucero Cuzcano Michael Papanicolaou Thomas R. Cox Stanley S. Stylli Theo Mantamadiotis |
| author_sort | Samuel S. Widodo |
| collection | DOAJ |
| description | Metastatic cancer is responsible for the overwhelming majority of cancer-related deaths, with metastatic tumors being the most common neoplasms affecting the central nervous system. One of the major factors regulating tumor biology is the tumor microenvironment. However, little is known about the cellular and non-cellular composition of metastatic brain tumors and how tumor cell ontogeny influences the metastatic brain tumor microenvironment. By integrating multiplex immunohistochemistry and histopathological analysis to investigate composition and the spatial relationship between neoplastic cells, infiltrating and brain resident immune cells and the extracellular matrix, we demonstrate that metastatic brain tumors exhibit differences in extracellular matrix deposition, compared with the most common primary brain tumor type, glioblastoma, and that the dominant immune cell types in metastatic brain tumors are immunosuppressive macrophages, which preferentially localize to extracellular matrix-rich stromal regions. |
| first_indexed | 2024-03-13T04:26:10Z |
| format | Article |
| id | doaj.art-b2b6fab43f1a48828905a3e7969b560a |
| institution | Directory Open Access Journal |
| issn | 2667-3940 |
| language | English |
| last_indexed | 2024-03-13T04:26:10Z |
| publishDate | 2023-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Advances in Cancer Biology - Metastasis |
| spelling | doaj.art-b2b6fab43f1a48828905a3e7969b560a2023-06-20T04:21:29ZengElsevierAdvances in Cancer Biology - Metastasis2667-39402023-07-017100096Spatial analysis of the metastatic brain tumor immune and extracellular matrix microenvironmentSamuel S. Widodo0Marija Dinevska1Lucero Cuzcano2Michael Papanicolaou3Thomas R. Cox4Stanley S. Stylli5Theo Mantamadiotis6Department of Surgery, Royal Melbourne Hospital, The University of Melbourne, Parkville, VIC, AustraliaDepartment of Surgery, Royal Melbourne Hospital, The University of Melbourne, Parkville, VIC, AustraliaDepartment of Surgery, Royal Melbourne Hospital, The University of Melbourne, Parkville, VIC, AustraliaThe Garvan Institute of Medical Research and the Kinghorn Cancer Centre, Cancer Ecosystems Program, Sydney, NSW, Australia; School of Life Sciences, Faculty of Science, University of Technology Sydney, Sydney, NSW, AustraliaThe Garvan Institute of Medical Research and the Kinghorn Cancer Centre, Cancer Ecosystems Program, Sydney, NSW, Australia; School of Clinical Medicine, St Vincent's Healthcare Clinical Campus, Faculty of Medicine and Health, UNSW Sydney, Sydney, NSW, AustraliaDepartment of Surgery, Royal Melbourne Hospital, The University of Melbourne, Parkville, VIC, Australia; Department of Neurosurgery, Royal Melbourne Hospital, Parkville, VIC, AustraliaDepartment of Surgery, Royal Melbourne Hospital, The University of Melbourne, Parkville, VIC, Australia; Department of Microbiology and Immunology, The University of Melbourne, Melbourne, VIC, Australia; Centre for Stem Cell Systems, The University of Melbourne, Parkville, VIC, Australia; Corresponding author. Department of Microbiology and Immunology, The University of Melbourne, VIC, Australia.Metastatic cancer is responsible for the overwhelming majority of cancer-related deaths, with metastatic tumors being the most common neoplasms affecting the central nervous system. One of the major factors regulating tumor biology is the tumor microenvironment. However, little is known about the cellular and non-cellular composition of metastatic brain tumors and how tumor cell ontogeny influences the metastatic brain tumor microenvironment. By integrating multiplex immunohistochemistry and histopathological analysis to investigate composition and the spatial relationship between neoplastic cells, infiltrating and brain resident immune cells and the extracellular matrix, we demonstrate that metastatic brain tumors exhibit differences in extracellular matrix deposition, compared with the most common primary brain tumor type, glioblastoma, and that the dominant immune cell types in metastatic brain tumors are immunosuppressive macrophages, which preferentially localize to extracellular matrix-rich stromal regions.http://www.sciencedirect.com/science/article/pii/S2667394023000102Brain cancerMetastatic brain cancerTumor microenvironmentMacrophagesT-cellsExtracellular matrix |
| spellingShingle | Samuel S. Widodo Marija Dinevska Lucero Cuzcano Michael Papanicolaou Thomas R. Cox Stanley S. Stylli Theo Mantamadiotis Spatial analysis of the metastatic brain tumor immune and extracellular matrix microenvironment Advances in Cancer Biology - Metastasis Brain cancer Metastatic brain cancer Tumor microenvironment Macrophages T-cells Extracellular matrix |
| title | Spatial analysis of the metastatic brain tumor immune and extracellular matrix microenvironment |
| title_full | Spatial analysis of the metastatic brain tumor immune and extracellular matrix microenvironment |
| title_fullStr | Spatial analysis of the metastatic brain tumor immune and extracellular matrix microenvironment |
| title_full_unstemmed | Spatial analysis of the metastatic brain tumor immune and extracellular matrix microenvironment |
| title_short | Spatial analysis of the metastatic brain tumor immune and extracellular matrix microenvironment |
| title_sort | spatial analysis of the metastatic brain tumor immune and extracellular matrix microenvironment |
| topic | Brain cancer Metastatic brain cancer Tumor microenvironment Macrophages T-cells Extracellular matrix |
| url | http://www.sciencedirect.com/science/article/pii/S2667394023000102 |
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