Contributions of protein microenvironment in tannase industrial applicability: An in-silico comparative study of pathogenic and non-pathogenic bacterial tannase
Tannase is an inducible industrially important enzyme, produced by several microorganisms. A large number of bacteria have reported as tannase producers; however, some of them are pathogenic in nature. Therefore, it is quite uncertain whether the application of these tannase enzymes from such pathog...
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| Format: | Article |
| Language: | English |
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Elsevier
2020-11-01
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| Series: | Heliyon |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844020322027 |
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| author | Ishita Biswas Debanjan Mitra Amal Kumar Bandyopadhyay Pradeep K. Das Mohapatra |
| author_facet | Ishita Biswas Debanjan Mitra Amal Kumar Bandyopadhyay Pradeep K. Das Mohapatra |
| author_sort | Ishita Biswas |
| collection | DOAJ |
| description | Tannase is an inducible industrially important enzyme, produced by several microorganisms. A large number of bacteria have reported as tannase producers; however, some of them are pathogenic in nature. Therefore, it is quite uncertain whether the application of these tannase enzymes from such pathogenic bacteria is suitable for industries and human welfare. Till date, there is no clear evidence regarding which group of bacteria (non-pathogenic or pathogenic) is better suited for their application in the edge of industries with particular reference to the food industry. The present study is following the findings of the above queries. In this study, a large number of tannase protein sequences have been retrieved from the databases, including both non-pathogenic and pathogenic bacterial species. Physiochemical and evolutionary properties of those sequences have been evaluated. Results have shown that non-pathogenic bacterial tannase possesses a high number of acidic and basic amino acid residues as compared to their pathogenic counterparts. The acidic and basic amino acid residues of tannase provide unique microenvironment to it. In the other hand, the numbers of disorder forming residues are higher in tannase sequences of pathogenic bacteria. The study of tannase microenvironment leads in the formation of salt bridges, which finally favoring the stability and proper functioning of tannase. This is the first report of such observation on tannase enzyme using in silico approach. Study of the microenvironment concept will be helpful in protein engineering. |
| first_indexed | 2024-12-16T11:44:48Z |
| format | Article |
| id | doaj.art-b2bddabaaf214dbe825c9e016cc7c93a |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-12-16T11:44:48Z |
| publishDate | 2020-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-b2bddabaaf214dbe825c9e016cc7c93a2022-12-21T22:32:51ZengElsevierHeliyon2405-84402020-11-01611e05359Contributions of protein microenvironment in tannase industrial applicability: An in-silico comparative study of pathogenic and non-pathogenic bacterial tannaseIshita Biswas0Debanjan Mitra1Amal Kumar Bandyopadhyay2Pradeep K. Das Mohapatra3Department of Microbiology, Raiganj University, Raiganj, 733134, Uttar Dinajpur, West Bengal, IndiaDepartment of Microbiology, Raiganj University, Raiganj, 733134, Uttar Dinajpur, West Bengal, IndiaDepartment of Biotechnology, University of Burdwan, Burdwan, 713104, West Bengal, India; Corresponding author.Department of Microbiology, Raiganj University, Raiganj, 733134, Uttar Dinajpur, West Bengal, India; Corresponding author.Tannase is an inducible industrially important enzyme, produced by several microorganisms. A large number of bacteria have reported as tannase producers; however, some of them are pathogenic in nature. Therefore, it is quite uncertain whether the application of these tannase enzymes from such pathogenic bacteria is suitable for industries and human welfare. Till date, there is no clear evidence regarding which group of bacteria (non-pathogenic or pathogenic) is better suited for their application in the edge of industries with particular reference to the food industry. The present study is following the findings of the above queries. In this study, a large number of tannase protein sequences have been retrieved from the databases, including both non-pathogenic and pathogenic bacterial species. Physiochemical and evolutionary properties of those sequences have been evaluated. Results have shown that non-pathogenic bacterial tannase possesses a high number of acidic and basic amino acid residues as compared to their pathogenic counterparts. The acidic and basic amino acid residues of tannase provide unique microenvironment to it. In the other hand, the numbers of disorder forming residues are higher in tannase sequences of pathogenic bacteria. The study of tannase microenvironment leads in the formation of salt bridges, which finally favoring the stability and proper functioning of tannase. This is the first report of such observation on tannase enzyme using in silico approach. Study of the microenvironment concept will be helpful in protein engineering.http://www.sciencedirect.com/science/article/pii/S2405844020322027BiochemistryBioinformaticsBiotechnologyMicrobiologyMolecular biologyProteins |
| spellingShingle | Ishita Biswas Debanjan Mitra Amal Kumar Bandyopadhyay Pradeep K. Das Mohapatra Contributions of protein microenvironment in tannase industrial applicability: An in-silico comparative study of pathogenic and non-pathogenic bacterial tannase Heliyon Biochemistry Bioinformatics Biotechnology Microbiology Molecular biology Proteins |
| title | Contributions of protein microenvironment in tannase industrial applicability: An in-silico comparative study of pathogenic and non-pathogenic bacterial tannase |
| title_full | Contributions of protein microenvironment in tannase industrial applicability: An in-silico comparative study of pathogenic and non-pathogenic bacterial tannase |
| title_fullStr | Contributions of protein microenvironment in tannase industrial applicability: An in-silico comparative study of pathogenic and non-pathogenic bacterial tannase |
| title_full_unstemmed | Contributions of protein microenvironment in tannase industrial applicability: An in-silico comparative study of pathogenic and non-pathogenic bacterial tannase |
| title_short | Contributions of protein microenvironment in tannase industrial applicability: An in-silico comparative study of pathogenic and non-pathogenic bacterial tannase |
| title_sort | contributions of protein microenvironment in tannase industrial applicability an in silico comparative study of pathogenic and non pathogenic bacterial tannase |
| topic | Biochemistry Bioinformatics Biotechnology Microbiology Molecular biology Proteins |
| url | http://www.sciencedirect.com/science/article/pii/S2405844020322027 |
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