Inhibition of Shiga toxin-converting bacteriophage development by novel antioxidant compounds
Oxidative stress may be the major cause of induction of Shiga toxin-converting (Stx) prophages from chromosomes of Shiga toxin-producing Escherichia coli (STEC) in human intestine. Thus, we aimed to test a series of novel antioxidant compounds for their activities against prophage induction, thus, p...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2018-01-01
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| Series: | Journal of Enzyme Inhibition and Medicinal Chemistry |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/14756366.2018.1444610 |
| _version_ | 1831840571248869376 |
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| author | Sylwia Bloch Bożena Nejman-Faleńczyk Karolina Pierzynowska Ewa Piotrowska Alicja Węgrzyn Christelle Marminon Zouhair Bouaziz Pascal Nebois Joachim Jose Marc Le Borgne Luciano Saso Grzegorz Węgrzyn |
| author_facet | Sylwia Bloch Bożena Nejman-Faleńczyk Karolina Pierzynowska Ewa Piotrowska Alicja Węgrzyn Christelle Marminon Zouhair Bouaziz Pascal Nebois Joachim Jose Marc Le Borgne Luciano Saso Grzegorz Węgrzyn |
| author_sort | Sylwia Bloch |
| collection | DOAJ |
| description | Oxidative stress may be the major cause of induction of Shiga toxin-converting (Stx) prophages from chromosomes of Shiga toxin-producing Escherichia coli (STEC) in human intestine. Thus, we aimed to test a series of novel antioxidant compounds for their activities against prophage induction, thus, preventing pathogenicity of STEC. Forty-six compounds (derivatives of carbazole, indazole, triazole, quinolone, ninhydrine, and indenoindole) were tested. Fifteen of them gave promising results and were further characterized. Eleven compounds had acceptable profiles in cytotoxicity tests with human HEK-293 and HDFa cell lines. Three of them (selected for molecular studies) prevent the prophage induction at the level of expression of specific phage genes. In bacterial cells treated with hydrogen peroxide, expression of genes involved in the oxidative stress response was significantly less efficient in the presence of the tested compounds. Therefore, they apparently reduce the oxidative stress, which prevents induction of Stx prophage in E. coli. |
| first_indexed | 2024-12-23T06:13:38Z |
| format | Article |
| id | doaj.art-b2be2db2912e4af19840c620534de956 |
| institution | Directory Open Access Journal |
| issn | 1475-6366 1475-6374 |
| language | English |
| last_indexed | 2024-12-23T06:13:38Z |
| publishDate | 2018-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Journal of Enzyme Inhibition and Medicinal Chemistry |
| spelling | doaj.art-b2be2db2912e4af19840c620534de9562022-12-21T17:57:23ZengTaylor & Francis GroupJournal of Enzyme Inhibition and Medicinal Chemistry1475-63661475-63742018-01-0133163965010.1080/14756366.2018.14446101444610Inhibition of Shiga toxin-converting bacteriophage development by novel antioxidant compoundsSylwia Bloch0Bożena Nejman-Faleńczyk1Karolina Pierzynowska2Ewa Piotrowska3Alicja Węgrzyn4Christelle Marminon5Zouhair Bouaziz6Pascal Nebois7Joachim Jose8Marc Le Borgne9Luciano Saso10Grzegorz Węgrzyn11Faculty of Biology, University of GdanskFaculty of Biology, University of GdanskFaculty of Biology, University of GdanskFaculty of Biology, University of GdanskInstitute of Biochemistry and Biophysics, Polish Academy of SciencesUniversité de Lyon, Université Claude Bernard Lyon 1, Faculté de Pharmacie - ISPB, EA 4446 Bioactive Molecules and Medicinal Chemistry, SFR Santé Lyon-Est CNRS UMS3453-INSERM US7Université de Lyon, Université Claude Bernard Lyon 1, Faculté de Pharmacie - ISPB, EA 4446 Bioactive Molecules and Medicinal Chemistry, SFR Santé Lyon-Est CNRS UMS3453-INSERM US7Université de Lyon, Université Claude Bernard Lyon 1, Faculté de Pharmacie - ISPB, EA 4446 Bioactive Molecules and Medicinal Chemistry, SFR Santé Lyon-Est CNRS UMS3453-INSERM US7Institut für Pharmazeutische und Medizinische Chemie, PharmaCampus, Westfälische Wilhelms-Universität MünsterUniversité de Lyon, Université Claude Bernard Lyon 1, Faculté de Pharmacie - ISPB, EA 4446 Bioactive Molecules and Medicinal Chemistry, SFR Santé Lyon-Est CNRS UMS3453-INSERM US7Sapienza UniversityFaculty of Biology, University of GdanskOxidative stress may be the major cause of induction of Shiga toxin-converting (Stx) prophages from chromosomes of Shiga toxin-producing Escherichia coli (STEC) in human intestine. Thus, we aimed to test a series of novel antioxidant compounds for their activities against prophage induction, thus, preventing pathogenicity of STEC. Forty-six compounds (derivatives of carbazole, indazole, triazole, quinolone, ninhydrine, and indenoindole) were tested. Fifteen of them gave promising results and were further characterized. Eleven compounds had acceptable profiles in cytotoxicity tests with human HEK-293 and HDFa cell lines. Three of them (selected for molecular studies) prevent the prophage induction at the level of expression of specific phage genes. In bacterial cells treated with hydrogen peroxide, expression of genes involved in the oxidative stress response was significantly less efficient in the presence of the tested compounds. Therefore, they apparently reduce the oxidative stress, which prevents induction of Stx prophage in E. coli.http://dx.doi.org/10.1080/14756366.2018.1444610Shiga toxin-producing Escherichia coliShiga toxin-converting bacteriophageoxidative stressantioxidantsheterocyclic compounds |
| spellingShingle | Sylwia Bloch Bożena Nejman-Faleńczyk Karolina Pierzynowska Ewa Piotrowska Alicja Węgrzyn Christelle Marminon Zouhair Bouaziz Pascal Nebois Joachim Jose Marc Le Borgne Luciano Saso Grzegorz Węgrzyn Inhibition of Shiga toxin-converting bacteriophage development by novel antioxidant compounds Journal of Enzyme Inhibition and Medicinal Chemistry Shiga toxin-producing Escherichia coli Shiga toxin-converting bacteriophage oxidative stress antioxidants heterocyclic compounds |
| title | Inhibition of Shiga toxin-converting bacteriophage development by novel antioxidant compounds |
| title_full | Inhibition of Shiga toxin-converting bacteriophage development by novel antioxidant compounds |
| title_fullStr | Inhibition of Shiga toxin-converting bacteriophage development by novel antioxidant compounds |
| title_full_unstemmed | Inhibition of Shiga toxin-converting bacteriophage development by novel antioxidant compounds |
| title_short | Inhibition of Shiga toxin-converting bacteriophage development by novel antioxidant compounds |
| title_sort | inhibition of shiga toxin converting bacteriophage development by novel antioxidant compounds |
| topic | Shiga toxin-producing Escherichia coli Shiga toxin-converting bacteriophage oxidative stress antioxidants heterocyclic compounds |
| url | http://dx.doi.org/10.1080/14756366.2018.1444610 |
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