Preparation of Curcumin-Loaded Liposomes and Evaluation of Their Skin Permeation and Pharmacodynamics
This study aimed to investigate the <em>in</em><em> vitro</em> skin permeation and <em>in vivo</em><em> </em>antineoplastic effect of curcumin by using liposomes as the transdermal drug-de...
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MDPI AG
2012-05-01
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| Series: | Molecules |
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| Online Access: | http://www.mdpi.com/1420-3049/17/5/5972 |
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| author | Lei Zhou Xuan Liu Ling Yuan Zhenghai Zhang Qingqing Wu Yan Chen |
| author_facet | Lei Zhou Xuan Liu Ling Yuan Zhenghai Zhang Qingqing Wu Yan Chen |
| author_sort | Lei Zhou |
| collection | DOAJ |
| description | This study aimed to investigate the <em>in</em><em> vitro</em> skin permeation and <em>in vivo</em><em> </em>antineoplastic effect of curcumin by using liposomes as the transdermal drug-delivery system. Soybean phospholipids (SPC), egg yolk phospholipids (EPC), and hydrogenated soybean phospholipids (HSPC) were selected for the preparation of different kinds of phospholipids composed of curcumin-loaded liposomes: C-SPC-L (curcumin-loaded SPC liposomes), C-EPC-L (curcumin-loaded EPC liposomes), and C-HSPC-L (curcumin-loaded HSPC liposomes). The physical properties of different lipsomes were investigated as follows: photon correlation spectroscopy revealed that the average particle sizes of the three types of curcumin-loaded liposomes were 82.37 ± 2.19 nm (C-SPC-L), 83.13 ± 4.89 nm (C-EPC-L), and 92.42 ± 4.56 nm (C-HSPC-L), respectively. The encapsulation efficiency values were found to be 82.32 ± 3.91%, 81.59 ± 2.38%, and 80.77 ± 4.12%, respectively. An <em>i</em><em>n vitro</em> skin penetration study indicated that C-SPC-L most significantly promoted drug permeation and deposition followed by C-EPC-L, C-HSPC-L, and curcumin solution. Moreover, C-SPC-L displayed the greatest ability of all loaded liposomes to inhibit the growth of B16BL6 melanoma cells. Therefore, the C-SPC-L were chosen for further pharmacodynamic evaluation. A significant effect on antimelanoma activity was observed with C-SPC-L, as compared to treatment with curcumin solution <em>in vivo</em>. These results suggest that C-SPC-L would be a promising transdermal carrier for curcumin in cancer treatment. |
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| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-12-20T17:19:33Z |
| publishDate | 2012-05-01 |
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| spelling | doaj.art-b2c5511c9c1b475e8e63d2ac69c596e22022-12-21T19:31:54ZengMDPI AGMolecules1420-30492012-05-011755972598710.3390/molecules17055972Preparation of Curcumin-Loaded Liposomes and Evaluation of Their Skin Permeation and PharmacodynamicsLei ZhouXuan LiuLing YuanZhenghai ZhangQingqing WuYan ChenThis study aimed to investigate the <em>in</em><em> vitro</em> skin permeation and <em>in vivo</em><em> </em>antineoplastic effect of curcumin by using liposomes as the transdermal drug-delivery system. Soybean phospholipids (SPC), egg yolk phospholipids (EPC), and hydrogenated soybean phospholipids (HSPC) were selected for the preparation of different kinds of phospholipids composed of curcumin-loaded liposomes: C-SPC-L (curcumin-loaded SPC liposomes), C-EPC-L (curcumin-loaded EPC liposomes), and C-HSPC-L (curcumin-loaded HSPC liposomes). The physical properties of different lipsomes were investigated as follows: photon correlation spectroscopy revealed that the average particle sizes of the three types of curcumin-loaded liposomes were 82.37 ± 2.19 nm (C-SPC-L), 83.13 ± 4.89 nm (C-EPC-L), and 92.42 ± 4.56 nm (C-HSPC-L), respectively. The encapsulation efficiency values were found to be 82.32 ± 3.91%, 81.59 ± 2.38%, and 80.77 ± 4.12%, respectively. An <em>i</em><em>n vitro</em> skin penetration study indicated that C-SPC-L most significantly promoted drug permeation and deposition followed by C-EPC-L, C-HSPC-L, and curcumin solution. Moreover, C-SPC-L displayed the greatest ability of all loaded liposomes to inhibit the growth of B16BL6 melanoma cells. Therefore, the C-SPC-L were chosen for further pharmacodynamic evaluation. A significant effect on antimelanoma activity was observed with C-SPC-L, as compared to treatment with curcumin solution <em>in vivo</em>. These results suggest that C-SPC-L would be a promising transdermal carrier for curcumin in cancer treatment.http://www.mdpi.com/1420-3049/17/5/5972curcuminphospholipidsliposomesskin penetrationantimelanoma activity |
| spellingShingle | Lei Zhou Xuan Liu Ling Yuan Zhenghai Zhang Qingqing Wu Yan Chen Preparation of Curcumin-Loaded Liposomes and Evaluation of Their Skin Permeation and Pharmacodynamics Molecules curcumin phospholipids liposomes skin penetration antimelanoma activity |
| title | Preparation of Curcumin-Loaded Liposomes and Evaluation of Their Skin Permeation and Pharmacodynamics |
| title_full | Preparation of Curcumin-Loaded Liposomes and Evaluation of Their Skin Permeation and Pharmacodynamics |
| title_fullStr | Preparation of Curcumin-Loaded Liposomes and Evaluation of Their Skin Permeation and Pharmacodynamics |
| title_full_unstemmed | Preparation of Curcumin-Loaded Liposomes and Evaluation of Their Skin Permeation and Pharmacodynamics |
| title_short | Preparation of Curcumin-Loaded Liposomes and Evaluation of Their Skin Permeation and Pharmacodynamics |
| title_sort | preparation of curcumin loaded liposomes and evaluation of their skin permeation and pharmacodynamics |
| topic | curcumin phospholipids liposomes skin penetration antimelanoma activity |
| url | http://www.mdpi.com/1420-3049/17/5/5972 |
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