Shoot organogenesis induction from genetically verified individuals of endangered bog pine (Pinus uncinata subsp. uliginosa)
The objective of this work was to apply selected genetic markers to verify the genetic purity of bog pine (Pinus uncinata subsp. uliginosa) individuals from the Borkovicka blata population in order to initiate in vitro cultures from them. Key factors of shoot organogenesis induction from seedling sh...
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| Format: | Article |
| Language: | English |
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Czech Academy of Agricultural Sciences
2010-08-01
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| Series: | Journal of Forest Science |
| Subjects: | |
| Online Access: | https://jfs.agriculturejournals.cz/artkey/jfs-201008-0001_shoot-organogenesis-induction-from-genetically-verified-individuals-of-endangered-bog-pine-pinus-uncinata-subs.php |
| _version_ | 1797898862587805696 |
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| author | H. Vejsadová M. Lukášová |
| author_facet | H. Vejsadová M. Lukášová |
| author_sort | H. Vejsadová |
| collection | DOAJ |
| description | The objective of this work was to apply selected genetic markers to verify the genetic purity of bog pine (Pinus uncinata subsp. uliginosa) individuals from the Borkovicka blata population in order to initiate in vitro cultures from them. Key factors of shoot organogenesis induction from seedling shoot apices were examined. The plastid DNA analysis was used as a molecular-genetic tool. The trnL-trnF region of chloroplast DNA was tested by PCR-RFLP method; furthermore, the chloroplast microsatellite region Pt41093 was also analysed. All the tested individuals from the studied population matched the Pinus uncinata subsp. uliginosa haplotype. In bog pine, regeneration and development of axillary shoots were significantly enhanced in the presence of 5 g・l-1 activated charcoal on the WV5 and WPM (Duchefa) media. The highest proliferation (28-30%) of short shoots (brachyblasts) was observed on the WV5 medium in the absence of benzyladenine (BA) or at its low concentration only (0.1 mg・l-1). |
| first_indexed | 2024-04-10T08:19:50Z |
| format | Article |
| id | doaj.art-b2c955c0744e49bca7c58e1a797a13b1 |
| institution | Directory Open Access Journal |
| issn | 1212-4834 1805-935X |
| language | English |
| last_indexed | 2024-04-10T08:19:50Z |
| publishDate | 2010-08-01 |
| publisher | Czech Academy of Agricultural Sciences |
| record_format | Article |
| series | Journal of Forest Science |
| spelling | doaj.art-b2c955c0744e49bca7c58e1a797a13b12023-02-23T03:42:10ZengCzech Academy of Agricultural SciencesJournal of Forest Science1212-48341805-935X2010-08-0156834134710.17221/107/2009-JFSjfs-201008-0001Shoot organogenesis induction from genetically verified individuals of endangered bog pine (Pinus uncinata subsp. uliginosa)H. Vejsadová0M. Lukášová1Department of Biodiversity, Silva Tarouca Institute for Landscape and Ornamental Gardening, Průhonice, Czech RepublicDepartment of Biodiversity, Silva Tarouca Institute for Landscape and Ornamental Gardening, Průhonice, Czech RepublicThe objective of this work was to apply selected genetic markers to verify the genetic purity of bog pine (Pinus uncinata subsp. uliginosa) individuals from the Borkovicka blata population in order to initiate in vitro cultures from them. Key factors of shoot organogenesis induction from seedling shoot apices were examined. The plastid DNA analysis was used as a molecular-genetic tool. The trnL-trnF region of chloroplast DNA was tested by PCR-RFLP method; furthermore, the chloroplast microsatellite region Pt41093 was also analysed. All the tested individuals from the studied population matched the Pinus uncinata subsp. uliginosa haplotype. In bog pine, regeneration and development of axillary shoots were significantly enhanced in the presence of 5 g・l-1 activated charcoal on the WV5 and WPM (Duchefa) media. The highest proliferation (28-30%) of short shoots (brachyblasts) was observed on the WV5 medium in the absence of benzyladenine (BA) or at its low concentration only (0.1 mg・l-1).https://jfs.agriculturejournals.cz/artkey/jfs-201008-0001_shoot-organogenesis-induction-from-genetically-verified-individuals-of-endangered-bog-pine-pinus-uncinata-subs.phpdna extractionin vitro regenerationleavesplastid dnaseedling shoot apices |
| spellingShingle | H. Vejsadová M. Lukášová Shoot organogenesis induction from genetically verified individuals of endangered bog pine (Pinus uncinata subsp. uliginosa) Journal of Forest Science dna extraction in vitro regeneration leaves plastid dna seedling shoot apices |
| title | Shoot organogenesis induction from genetically verified individuals of endangered bog pine (Pinus uncinata subsp. uliginosa) |
| title_full | Shoot organogenesis induction from genetically verified individuals of endangered bog pine (Pinus uncinata subsp. uliginosa) |
| title_fullStr | Shoot organogenesis induction from genetically verified individuals of endangered bog pine (Pinus uncinata subsp. uliginosa) |
| title_full_unstemmed | Shoot organogenesis induction from genetically verified individuals of endangered bog pine (Pinus uncinata subsp. uliginosa) |
| title_short | Shoot organogenesis induction from genetically verified individuals of endangered bog pine (Pinus uncinata subsp. uliginosa) |
| title_sort | shoot organogenesis induction from genetically verified individuals of endangered bog pine pinus uncinata subsp uliginosa |
| topic | dna extraction in vitro regeneration leaves plastid dna seedling shoot apices |
| url | https://jfs.agriculturejournals.cz/artkey/jfs-201008-0001_shoot-organogenesis-induction-from-genetically-verified-individuals-of-endangered-bog-pine-pinus-uncinata-subs.php |
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