Assessment of Iris albicans lange as potential antimicrobial and analgesic agent.
Objective of the present work was to evaluate the presence of phytochemical constituents and pharmacological activities (antimicrobial and anti-nociceptive) of crude extract isolated from Iris albicans and its corresponding fractions. Extraction was made with methanol and extract was evaluated for t...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2023-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0280127 |
| _version_ | 1797866048642351104 |
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| author | Muhammad Bilal Attiqa Naz Amjad Khan Salman Rukhsana Ghaffar Azra Abrar |
| author_facet | Muhammad Bilal Attiqa Naz Amjad Khan Salman Rukhsana Ghaffar Azra Abrar |
| author_sort | Muhammad Bilal |
| collection | DOAJ |
| description | Objective of the present work was to evaluate the presence of phytochemical constituents and pharmacological activities (antimicrobial and anti-nociceptive) of crude extract isolated from Iris albicans and its corresponding fractions. Extraction was made with methanol and extract was evaluated for the presence of different bioactive constituents, as per standard protocols. Extract and its corresponding fractions were evaluated for their antimicrobial and anti-nociceptive potential. Well diffusion method was used to evaluate the antibacterial effects while anti-nociceptive effect was studied using in-vivo models (acetic acid induced writhing, hotplate and tail immersion tests) at different dose level (100, 200 and 300 mg/kg of body weight) and compared with diclofenac sodium (dose = 10 mg/kg body weight). Results showed that the I. albicans extracts contained secondary metabolites including alkaloids, phytosterols, flavonoids, saponins, terpenoids, tannins, phenols, steroids, fixed oil, glycosides and carbohydrates. Furthermore, it was observed that different fractions in decreasing polarity order such as chloroform >n-Hexane > Ethyl acetate > Crude Methanol > Aqueous extract exhibited effective antimicrobial response against all test organisms. Results of the study showed that the extracts have significant antimicrobial and analgesic activity, providing evidence for its folklore use. |
| first_indexed | 2024-04-09T23:17:51Z |
| format | Article |
| id | doaj.art-b2cb8dd537d1477981ff998670e0fdb5 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-09T23:17:51Z |
| publishDate | 2023-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-b2cb8dd537d1477981ff998670e0fdb52023-03-22T05:31:23ZengPublic Library of Science (PLoS)PLoS ONE1932-62032023-01-01181e028012710.1371/journal.pone.0280127Assessment of Iris albicans lange as potential antimicrobial and analgesic agent.Muhammad BilalAttiqa NazAmjad KhanSalmanRukhsana GhaffarAzra AbrarObjective of the present work was to evaluate the presence of phytochemical constituents and pharmacological activities (antimicrobial and anti-nociceptive) of crude extract isolated from Iris albicans and its corresponding fractions. Extraction was made with methanol and extract was evaluated for the presence of different bioactive constituents, as per standard protocols. Extract and its corresponding fractions were evaluated for their antimicrobial and anti-nociceptive potential. Well diffusion method was used to evaluate the antibacterial effects while anti-nociceptive effect was studied using in-vivo models (acetic acid induced writhing, hotplate and tail immersion tests) at different dose level (100, 200 and 300 mg/kg of body weight) and compared with diclofenac sodium (dose = 10 mg/kg body weight). Results showed that the I. albicans extracts contained secondary metabolites including alkaloids, phytosterols, flavonoids, saponins, terpenoids, tannins, phenols, steroids, fixed oil, glycosides and carbohydrates. Furthermore, it was observed that different fractions in decreasing polarity order such as chloroform >n-Hexane > Ethyl acetate > Crude Methanol > Aqueous extract exhibited effective antimicrobial response against all test organisms. Results of the study showed that the extracts have significant antimicrobial and analgesic activity, providing evidence for its folklore use.https://doi.org/10.1371/journal.pone.0280127 |
| spellingShingle | Muhammad Bilal Attiqa Naz Amjad Khan Salman Rukhsana Ghaffar Azra Abrar Assessment of Iris albicans lange as potential antimicrobial and analgesic agent. PLoS ONE |
| title | Assessment of Iris albicans lange as potential antimicrobial and analgesic agent. |
| title_full | Assessment of Iris albicans lange as potential antimicrobial and analgesic agent. |
| title_fullStr | Assessment of Iris albicans lange as potential antimicrobial and analgesic agent. |
| title_full_unstemmed | Assessment of Iris albicans lange as potential antimicrobial and analgesic agent. |
| title_short | Assessment of Iris albicans lange as potential antimicrobial and analgesic agent. |
| title_sort | assessment of iris albicans lange as potential antimicrobial and analgesic agent |
| url | https://doi.org/10.1371/journal.pone.0280127 |
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