Prebiotic Potential of Agave angustifolia Haw Fructans with Different Degrees of Polymerization
Inulin-type fructans are the most studied prebiotic compounds because of their broad range of health benefits. In particular, plants of the Agave genus are rich in fructans. Agave-derived fructans have a branched structure with both β-(2→1) and β-(2→6) linked fructosyl chains attached to the sucrose...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2014-08-01
|
| Series: | Molecules |
| Subjects: | |
| Online Access: | http://www.mdpi.com/1420-3049/19/8/12660 |
| _version_ | 1819028640321503232 |
|---|---|
| author | José Rodolfo Velázquez-Martínez Rina M. González-Cervantes Minerva Aurora Hernández-Gallegos Roberto Campos Mendiola Antonio R. Jiménez Aparicio Martha L. Arenas Ocampo |
| author_facet | José Rodolfo Velázquez-Martínez Rina M. González-Cervantes Minerva Aurora Hernández-Gallegos Roberto Campos Mendiola Antonio R. Jiménez Aparicio Martha L. Arenas Ocampo |
| author_sort | José Rodolfo Velázquez-Martínez |
| collection | DOAJ |
| description | Inulin-type fructans are the most studied prebiotic compounds because of their broad range of health benefits. In particular, plants of the Agave genus are rich in fructans. Agave-derived fructans have a branched structure with both β-(2→1) and β-(2→6) linked fructosyl chains attached to the sucrose start unit with a degree of polymerization (DP) of up to 80 fructose units. The objective of this work was to assess the prebiotic potential of three Agave angustifolia Haw fructan fractions (AFF) with different degrees of polymerization. The three fructan fractions were extracted from the agave stem by lixiviation and then purified by ultrafiltration and ion exchange chromatography: AFF1, AFF2 and AFF3 with high (3–60 fructose units), medium (2–40) and low (2–22) DP, respectively. The fructan profile was determined with high-performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD), which confirmed a branched fructan structure. Structural elucidation was performed by Fourier Transform Infra-Red Spectroscopy. The AFF spectrum shows characteristic fructan bands. The prebiotic effect of these fractions was assessed in vitro through fermentation by Bifidobacterium and Lactobacillus strains. Four growth patterns were observed. Some bacteria did not grow with any of the AFF, while other strains grew with only AFF3. Some bacteria grew according to the molecular weight of the AFF and some grew indistinctly with the three fructan fractions. |
| first_indexed | 2024-12-21T06:01:35Z |
| format | Article |
| id | doaj.art-b2cf032a56f1404883ea9b5be3c52e32 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-12-21T06:01:35Z |
| publishDate | 2014-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-b2cf032a56f1404883ea9b5be3c52e322022-12-21T19:13:45ZengMDPI AGMolecules1420-30492014-08-01198126601267510.3390/molecules190812660molecules190812660Prebiotic Potential of Agave angustifolia Haw Fructans with Different Degrees of PolymerizationJosé Rodolfo Velázquez-Martínez0Rina M. González-Cervantes1Minerva Aurora Hernández-Gallegos2Roberto Campos Mendiola3Antonio R. Jiménez Aparicio4Martha L. Arenas Ocampo5Centro de Desarrollo de Productos Bióticos, Instituto Politécnico Nacional, P.O. Box 24, Yautepec 62730, Morelos, MexicoDepartamento de Sistemas Biológicos, Universidad Autónoma Metropolitana-Xochimilco, Calzada del Hueso 1100, Col. Villa Quietud, Delegación Coyoacán 04960, D.F., MexicoEscuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Prolongación de Carpio y Plan de Ayala s/n, Col. Santo Tomas, Delegación Miguel Hidalgo 11340, D.F., MexicoCentro de Desarrollo de Productos Bióticos, Instituto Politécnico Nacional, P.O. Box 24, Yautepec 62730, Morelos, MexicoCentro de Desarrollo de Productos Bióticos, Instituto Politécnico Nacional, P.O. Box 24, Yautepec 62730, Morelos, MexicoCentro de Desarrollo de Productos Bióticos, Instituto Politécnico Nacional, P.O. Box 24, Yautepec 62730, Morelos, MexicoInulin-type fructans are the most studied prebiotic compounds because of their broad range of health benefits. In particular, plants of the Agave genus are rich in fructans. Agave-derived fructans have a branched structure with both β-(2→1) and β-(2→6) linked fructosyl chains attached to the sucrose start unit with a degree of polymerization (DP) of up to 80 fructose units. The objective of this work was to assess the prebiotic potential of three Agave angustifolia Haw fructan fractions (AFF) with different degrees of polymerization. The three fructan fractions were extracted from the agave stem by lixiviation and then purified by ultrafiltration and ion exchange chromatography: AFF1, AFF2 and AFF3 with high (3–60 fructose units), medium (2–40) and low (2–22) DP, respectively. The fructan profile was determined with high-performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD), which confirmed a branched fructan structure. Structural elucidation was performed by Fourier Transform Infra-Red Spectroscopy. The AFF spectrum shows characteristic fructan bands. The prebiotic effect of these fractions was assessed in vitro through fermentation by Bifidobacterium and Lactobacillus strains. Four growth patterns were observed. Some bacteria did not grow with any of the AFF, while other strains grew with only AFF3. Some bacteria grew according to the molecular weight of the AFF and some grew indistinctly with the three fructan fractions.http://www.mdpi.com/1420-3049/19/8/12660BifidobacteriumLactobacillusAgave angustifolia Hawprebioticfructans |
| spellingShingle | José Rodolfo Velázquez-Martínez Rina M. González-Cervantes Minerva Aurora Hernández-Gallegos Roberto Campos Mendiola Antonio R. Jiménez Aparicio Martha L. Arenas Ocampo Prebiotic Potential of Agave angustifolia Haw Fructans with Different Degrees of Polymerization Molecules Bifidobacterium Lactobacillus Agave angustifolia Haw prebiotic fructans |
| title | Prebiotic Potential of Agave angustifolia Haw Fructans with Different Degrees of Polymerization |
| title_full | Prebiotic Potential of Agave angustifolia Haw Fructans with Different Degrees of Polymerization |
| title_fullStr | Prebiotic Potential of Agave angustifolia Haw Fructans with Different Degrees of Polymerization |
| title_full_unstemmed | Prebiotic Potential of Agave angustifolia Haw Fructans with Different Degrees of Polymerization |
| title_short | Prebiotic Potential of Agave angustifolia Haw Fructans with Different Degrees of Polymerization |
| title_sort | prebiotic potential of agave angustifolia haw fructans with different degrees of polymerization |
| topic | Bifidobacterium Lactobacillus Agave angustifolia Haw prebiotic fructans |
| url | http://www.mdpi.com/1420-3049/19/8/12660 |
| work_keys_str_mv | AT joserodolfovelazquezmartinez prebioticpotentialofagaveangustifoliahawfructanswithdifferentdegreesofpolymerization AT rinamgonzalezcervantes prebioticpotentialofagaveangustifoliahawfructanswithdifferentdegreesofpolymerization AT minervaaurorahernandezgallegos prebioticpotentialofagaveangustifoliahawfructanswithdifferentdegreesofpolymerization AT robertocamposmendiola prebioticpotentialofagaveangustifoliahawfructanswithdifferentdegreesofpolymerization AT antoniorjimenezaparicio prebioticpotentialofagaveangustifoliahawfructanswithdifferentdegreesofpolymerization AT marthalarenasocampo prebioticpotentialofagaveangustifoliahawfructanswithdifferentdegreesofpolymerization |