Genomic Insights into Drug Resistance Determinants in <i>Cedecea neteri</i>, A Rare Opportunistic Pathogen
<i>Cedecea</i>, a genus in the <i>Enterobacteriaceae</i> family, includes several opportunistic pathogens reported to cause an array of sporadic acute infections, most notably of the lung and bloodstream. One species, <i>Cedecea neteri</i>, is associated with case...
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MDPI AG
2021-08-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/9/8/1741 |
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| author | Dorothea K. Thompson Stephen M. Sharkady |
| author_facet | Dorothea K. Thompson Stephen M. Sharkady |
| author_sort | Dorothea K. Thompson |
| collection | DOAJ |
| description | <i>Cedecea</i>, a genus in the <i>Enterobacteriaceae</i> family, includes several opportunistic pathogens reported to cause an array of sporadic acute infections, most notably of the lung and bloodstream. One species, <i>Cedecea neteri</i>, is associated with cases of bacteremia in immunocompromised hosts and has documented resistance to different antibiotics, including β-lactams and colistin. Despite the potential to inflict serious infections, knowledge about drug resistance determinants in <i>Cedecea</i> is limited. In this study, we utilized whole-genome sequence data available for three environmental strains (SSMD04, M006, ND14a) of <i>C. neteri</i> and various bioinformatics tools to analyze drug resistance genes in this bacterium. All three genomes harbor multiple chromosome-encoded β-lactamase genes. A deeper analysis of β-lactamase genes in SSMD04 revealed four metallo-β-lactamases, a novel variant, and a CMY/ACT-type AmpC putatively regulated by a divergently transcribed AmpR. Homologs of known resistance-nodulation-cell division (RND)-type multidrug efflux pumps such as OqxB, AcrB, AcrD, and MdtBC were also identified. Genomic island prediction for SSMD04 indicated that <i>tolC</i>, involved in drug and toxin export across the outer membrane of Gram-negative bacteria, was acquired by a transposase-mediated genetic transfer mechanism. Our study provides new insights into drug resistance mechanisms of an environmental microorganism capable of behaving as a clinically relevant opportunistic pathogen. |
| first_indexed | 2024-03-10T08:34:28Z |
| format | Article |
| id | doaj.art-b2cfa20d4a0e4adb8b0e9b397292a046 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T08:34:28Z |
| publishDate | 2021-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-b2cfa20d4a0e4adb8b0e9b397292a0462023-11-22T08:48:11ZengMDPI AGMicroorganisms2076-26072021-08-0198174110.3390/microorganisms9081741Genomic Insights into Drug Resistance Determinants in <i>Cedecea neteri</i>, A Rare Opportunistic PathogenDorothea K. Thompson0Stephen M. Sharkady1Department of Pharmaceutical Sciences, College of Pharmacy & Health Sciences, Campbell University, Buies Creek, NC 27506, USADepartment of Pharmaceutical Sciences, College of Pharmacy & Health Sciences, Campbell University, Buies Creek, NC 27506, USA<i>Cedecea</i>, a genus in the <i>Enterobacteriaceae</i> family, includes several opportunistic pathogens reported to cause an array of sporadic acute infections, most notably of the lung and bloodstream. One species, <i>Cedecea neteri</i>, is associated with cases of bacteremia in immunocompromised hosts and has documented resistance to different antibiotics, including β-lactams and colistin. Despite the potential to inflict serious infections, knowledge about drug resistance determinants in <i>Cedecea</i> is limited. In this study, we utilized whole-genome sequence data available for three environmental strains (SSMD04, M006, ND14a) of <i>C. neteri</i> and various bioinformatics tools to analyze drug resistance genes in this bacterium. All three genomes harbor multiple chromosome-encoded β-lactamase genes. A deeper analysis of β-lactamase genes in SSMD04 revealed four metallo-β-lactamases, a novel variant, and a CMY/ACT-type AmpC putatively regulated by a divergently transcribed AmpR. Homologs of known resistance-nodulation-cell division (RND)-type multidrug efflux pumps such as OqxB, AcrB, AcrD, and MdtBC were also identified. Genomic island prediction for SSMD04 indicated that <i>tolC</i>, involved in drug and toxin export across the outer membrane of Gram-negative bacteria, was acquired by a transposase-mediated genetic transfer mechanism. Our study provides new insights into drug resistance mechanisms of an environmental microorganism capable of behaving as a clinically relevant opportunistic pathogen.https://www.mdpi.com/2076-2607/9/8/1741<i>Cedecea neteri</i>opportunistic pathogendrug-resistance genesβ-lactamasesmultidrug efflux pumpsgenomic islands |
| spellingShingle | Dorothea K. Thompson Stephen M. Sharkady Genomic Insights into Drug Resistance Determinants in <i>Cedecea neteri</i>, A Rare Opportunistic Pathogen Microorganisms <i>Cedecea neteri</i> opportunistic pathogen drug-resistance genes β-lactamases multidrug efflux pumps genomic islands |
| title | Genomic Insights into Drug Resistance Determinants in <i>Cedecea neteri</i>, A Rare Opportunistic Pathogen |
| title_full | Genomic Insights into Drug Resistance Determinants in <i>Cedecea neteri</i>, A Rare Opportunistic Pathogen |
| title_fullStr | Genomic Insights into Drug Resistance Determinants in <i>Cedecea neteri</i>, A Rare Opportunistic Pathogen |
| title_full_unstemmed | Genomic Insights into Drug Resistance Determinants in <i>Cedecea neteri</i>, A Rare Opportunistic Pathogen |
| title_short | Genomic Insights into Drug Resistance Determinants in <i>Cedecea neteri</i>, A Rare Opportunistic Pathogen |
| title_sort | genomic insights into drug resistance determinants in i cedecea neteri i a rare opportunistic pathogen |
| topic | <i>Cedecea neteri</i> opportunistic pathogen drug-resistance genes β-lactamases multidrug efflux pumps genomic islands |
| url | https://www.mdpi.com/2076-2607/9/8/1741 |
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