SOX2 and SOX9 Expression in Developing Postnatal Opossum (<i>Monodelphis domestica</i>) Cortex
(1) Background: Central nervous system (CNS) development is characterized by dynamic changes in cell proliferation and differentiation. Key regulators of these transitions are the transcription factors such as SOX2 and SOX9. SOX2 is involved in the maintenance of progenitor cell state and neural ste...
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MDPI AG
2024-01-01
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| Series: | Biomolecules |
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| Online Access: | https://www.mdpi.com/2218-273X/14/1/70 |
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| author | Zrinko Baričević Marta Pongrac Matea Ivaničić Helena Hreščak Ivana Tomljanović Antonela Petrović Dan Cojoc Miranda Mladinic Jelena Ban |
| author_facet | Zrinko Baričević Marta Pongrac Matea Ivaničić Helena Hreščak Ivana Tomljanović Antonela Petrović Dan Cojoc Miranda Mladinic Jelena Ban |
| author_sort | Zrinko Baričević |
| collection | DOAJ |
| description | (1) Background: Central nervous system (CNS) development is characterized by dynamic changes in cell proliferation and differentiation. Key regulators of these transitions are the transcription factors such as SOX2 and SOX9. SOX2 is involved in the maintenance of progenitor cell state and neural stem cell multipotency, while SOX9, expressed in neurogenic niches, plays an important role in neuron/glia switch with predominant expression in astrocytes in the adult brain. (2) Methods: To validate SOX2 and SOX9 expression patterns in developing opossum (<i>Monodelphis domestica</i>) cortex, we used immunohistochemistry (IHC) and the isotropic fractionator method on fixed cortical tissue from comparable postnatal ages, as well as dissociated primary neuronal cultures. (3) Results: Neurons positive for both neuronal (TUJ1 or NeuN) and stem cell (SOX2) markers were identified, and their presence was confirmed with all methods and postnatal age groups (P4-6, P6-18, and P30) analyzed. SOX9 showed exclusive staining in non-neuronal cells, and it was coexpressed with SOX2. (4) Conclusions: The persistence of SOX2 expression in developing cortical neurons of <i>M. domestica</i> during the first postnatal month implies the functional role of SOX2 during neuronal differentiation and maturation, which was not previously reported in opossums. |
| first_indexed | 2024-03-08T11:04:40Z |
| format | Article |
| id | doaj.art-b2cfa65619fb4035b6a7026f5c055f00 |
| institution | Directory Open Access Journal |
| issn | 2218-273X |
| language | English |
| last_indexed | 2024-03-08T11:04:40Z |
| publishDate | 2024-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomolecules |
| spelling | doaj.art-b2cfa65619fb4035b6a7026f5c055f002024-01-26T15:19:17ZengMDPI AGBiomolecules2218-273X2024-01-011417010.3390/biom14010070SOX2 and SOX9 Expression in Developing Postnatal Opossum (<i>Monodelphis domestica</i>) CortexZrinko Baričević0Marta Pongrac1Matea Ivaničić2Helena Hreščak3Ivana Tomljanović4Antonela Petrović5Dan Cojoc6Miranda Mladinic7Jelena Ban8Faculty of Biotechnology and Drug Development, University of Rijeka, Radmile Matejčić 2, 51000 Rijeka, CroatiaFaculty of Biotechnology and Drug Development, University of Rijeka, Radmile Matejčić 2, 51000 Rijeka, CroatiaFaculty of Biotechnology and Drug Development, University of Rijeka, Radmile Matejčić 2, 51000 Rijeka, CroatiaFaculty of Biotechnology and Drug Development, University of Rijeka, Radmile Matejčić 2, 51000 Rijeka, CroatiaFaculty of Biotechnology and Drug Development, University of Rijeka, Radmile Matejčić 2, 51000 Rijeka, CroatiaFaculty of Biotechnology and Drug Development, University of Rijeka, Radmile Matejčić 2, 51000 Rijeka, CroatiaCNR-IOM, Materials Foundry, National Research Council of Italy, 34149 Trieste, ItalyFaculty of Biotechnology and Drug Development, University of Rijeka, Radmile Matejčić 2, 51000 Rijeka, CroatiaFaculty of Biotechnology and Drug Development, University of Rijeka, Radmile Matejčić 2, 51000 Rijeka, Croatia(1) Background: Central nervous system (CNS) development is characterized by dynamic changes in cell proliferation and differentiation. Key regulators of these transitions are the transcription factors such as SOX2 and SOX9. SOX2 is involved in the maintenance of progenitor cell state and neural stem cell multipotency, while SOX9, expressed in neurogenic niches, plays an important role in neuron/glia switch with predominant expression in astrocytes in the adult brain. (2) Methods: To validate SOX2 and SOX9 expression patterns in developing opossum (<i>Monodelphis domestica</i>) cortex, we used immunohistochemistry (IHC) and the isotropic fractionator method on fixed cortical tissue from comparable postnatal ages, as well as dissociated primary neuronal cultures. (3) Results: Neurons positive for both neuronal (TUJ1 or NeuN) and stem cell (SOX2) markers were identified, and their presence was confirmed with all methods and postnatal age groups (P4-6, P6-18, and P30) analyzed. SOX9 showed exclusive staining in non-neuronal cells, and it was coexpressed with SOX2. (4) Conclusions: The persistence of SOX2 expression in developing cortical neurons of <i>M. domestica</i> during the first postnatal month implies the functional role of SOX2 during neuronal differentiation and maturation, which was not previously reported in opossums.https://www.mdpi.com/2218-273X/14/1/70opossumcortexdevelopmentneurogenesisprimary neuronal culturesisotropic fractionator |
| spellingShingle | Zrinko Baričević Marta Pongrac Matea Ivaničić Helena Hreščak Ivana Tomljanović Antonela Petrović Dan Cojoc Miranda Mladinic Jelena Ban SOX2 and SOX9 Expression in Developing Postnatal Opossum (<i>Monodelphis domestica</i>) Cortex Biomolecules opossum cortex development neurogenesis primary neuronal cultures isotropic fractionator |
| title | SOX2 and SOX9 Expression in Developing Postnatal Opossum (<i>Monodelphis domestica</i>) Cortex |
| title_full | SOX2 and SOX9 Expression in Developing Postnatal Opossum (<i>Monodelphis domestica</i>) Cortex |
| title_fullStr | SOX2 and SOX9 Expression in Developing Postnatal Opossum (<i>Monodelphis domestica</i>) Cortex |
| title_full_unstemmed | SOX2 and SOX9 Expression in Developing Postnatal Opossum (<i>Monodelphis domestica</i>) Cortex |
| title_short | SOX2 and SOX9 Expression in Developing Postnatal Opossum (<i>Monodelphis domestica</i>) Cortex |
| title_sort | sox2 and sox9 expression in developing postnatal opossum i monodelphis domestica i cortex |
| topic | opossum cortex development neurogenesis primary neuronal cultures isotropic fractionator |
| url | https://www.mdpi.com/2218-273X/14/1/70 |
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