The mechanism of enhanced lignin regulating foliar Cd absorption and yield in rice (Oryza sativa L.)
The impact of atmospheric deposition of cadmium (Cd) in cereal crops has become a global concern. Enhanced lignin content was expected to benefit the plant performance against Cd exposure. To date, however, the underlying mechanisms of lignin regulating foliar Cd absorption in rice (Oryza sativa L.)...
| Main Authors: | , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-01-01
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| Series: | Ecotoxicology and Environmental Safety |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0147651322013215 |
| _version_ | 1828071626357342208 |
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| author | Qin Dong Qi Tao Bing Li Rong Huang Qiang Xu Huanxiu Li Jie Shen Xi Chen Qiquan Li Xiaoyan Tang František Kačík Ján Kováč Jaroslav Ďurkovič Yingjie Wu Changquan Wang |
| author_facet | Qin Dong Qi Tao Bing Li Rong Huang Qiang Xu Huanxiu Li Jie Shen Xi Chen Qiquan Li Xiaoyan Tang František Kačík Ján Kováč Jaroslav Ďurkovič Yingjie Wu Changquan Wang |
| author_sort | Qin Dong |
| collection | DOAJ |
| description | The impact of atmospheric deposition of cadmium (Cd) in cereal crops has become a global concern. Enhanced lignin content was expected to benefit the plant performance against Cd exposure. To date, however, the underlying mechanisms of lignin regulating foliar Cd absorption in rice (Oryza sativa L.) and its effect on grain yield remains unclear. In present study, the effect and mechanism of rice in response to leaf Cd exposure were investigated using 113Cd stable isotope and a lignin-increased rice mutant. The highest Cd uptake efficiency and uptake amount was observed in wild type (WT) plant grown in the maturity period, which were 3-fold higher than in mutant plant. Compared to WT, the mutant exhibited 14.75% and 25.43% higher contents in G- and S-unit of lignin monomers. Lignin biosynthesis and polymerization related genes (OsPAL/OsCOMT/Os4CL3/OsLAC5/OsLAC15) were significantly up-regulated in mutants. In addition, the enzyme activities involved in the above process were also significantly increased by 1.24–1.49-fold. The increased Cd retention in cell wall and decreased gene expression levels of OsNRAMP5, OsHMA3 and OsIRT1 in mutant indicated that lignin effectively inhibited Cd transportion in plant tissues. Moreover, the antioxidant capacity and photosynthesis efficiency in mutant plant were obviously improved, leading to higher Cd tolerance and increased grain yield. Our results revealed the molecular and physiological mechanisms of enhanced lignin regulating foliar Cd absorption and yield in rice, and provided the valuable rice genotype to ensure food safety. |
| first_indexed | 2024-04-11T00:56:50Z |
| format | Article |
| id | doaj.art-b2d3a4a5310147508da8f2c32be16f35 |
| institution | Directory Open Access Journal |
| issn | 0147-6513 |
| language | English |
| last_indexed | 2024-04-11T00:56:50Z |
| publishDate | 2023-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ecotoxicology and Environmental Safety |
| spelling | doaj.art-b2d3a4a5310147508da8f2c32be16f352023-01-05T04:30:55ZengElsevierEcotoxicology and Environmental Safety0147-65132023-01-01249114481The mechanism of enhanced lignin regulating foliar Cd absorption and yield in rice (Oryza sativa L.)Qin Dong0Qi Tao1Bing Li2Rong Huang3Qiang Xu4Huanxiu Li5Jie Shen6Xi Chen7Qiquan Li8Xiaoyan Tang9František Kačík10Ján Kováč11Jaroslav Ďurkovič12Yingjie Wu13Changquan Wang14College of Resources, Sichuan Agricultural University, Chengdu 611130, ChinaCollege of Resources, Sichuan Agricultural University, Chengdu 611130, ChinaCollege of Resources, Sichuan Agricultural University, Chengdu 611130, ChinaCollege of Resources, Sichuan Agricultural University, Chengdu 611130, ChinaCollege of Resources, Sichuan Agricultural University, Chengdu 611130, ChinaCollege of Resources, Sichuan Agricultural University, Chengdu 611130, ChinaChina-Croatia “Belt and Road” Joint Laboratory on Biodiversity and Ecosystem Services, CAS Key Laboratory of Mountain Ecological Restoration and Bioresource Utilization & Ecological Restoration and Biodiversity Conservation Key Laboratory of Sichuan Province, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, ChinaCollege of Resources, Sichuan Agricultural University, Chengdu 611130, ChinaCollege of Resources, Sichuan Agricultural University, Chengdu 611130, ChinaCollege of Resources, Sichuan Agricultural University, Chengdu 611130, ChinaDepartment of Chemistry and Chemical Technologies, Technical University in Zvolen, 96001 Zvolen, SlovakiaDepartment of Phytology, Technical University in Zvolen, 96001 Zvolen, SlovakiaDepartment of Phytology, Technical University in Zvolen, 96001 Zvolen, SlovakiaCollege of Resources, Sichuan Agricultural University, Chengdu 611130, China; Corresponding authors.College of Resources, Sichuan Agricultural University, Chengdu 611130, China; Corresponding authors.The impact of atmospheric deposition of cadmium (Cd) in cereal crops has become a global concern. Enhanced lignin content was expected to benefit the plant performance against Cd exposure. To date, however, the underlying mechanisms of lignin regulating foliar Cd absorption in rice (Oryza sativa L.) and its effect on grain yield remains unclear. In present study, the effect and mechanism of rice in response to leaf Cd exposure were investigated using 113Cd stable isotope and a lignin-increased rice mutant. The highest Cd uptake efficiency and uptake amount was observed in wild type (WT) plant grown in the maturity period, which were 3-fold higher than in mutant plant. Compared to WT, the mutant exhibited 14.75% and 25.43% higher contents in G- and S-unit of lignin monomers. Lignin biosynthesis and polymerization related genes (OsPAL/OsCOMT/Os4CL3/OsLAC5/OsLAC15) were significantly up-regulated in mutants. In addition, the enzyme activities involved in the above process were also significantly increased by 1.24–1.49-fold. The increased Cd retention in cell wall and decreased gene expression levels of OsNRAMP5, OsHMA3 and OsIRT1 in mutant indicated that lignin effectively inhibited Cd transportion in plant tissues. Moreover, the antioxidant capacity and photosynthesis efficiency in mutant plant were obviously improved, leading to higher Cd tolerance and increased grain yield. Our results revealed the molecular and physiological mechanisms of enhanced lignin regulating foliar Cd absorption and yield in rice, and provided the valuable rice genotype to ensure food safety.http://www.sciencedirect.com/science/article/pii/S0147651322013215Heavy metalOryza sativaFood safetyStable isotope tracerGenetic engineering |
| spellingShingle | Qin Dong Qi Tao Bing Li Rong Huang Qiang Xu Huanxiu Li Jie Shen Xi Chen Qiquan Li Xiaoyan Tang František Kačík Ján Kováč Jaroslav Ďurkovič Yingjie Wu Changquan Wang The mechanism of enhanced lignin regulating foliar Cd absorption and yield in rice (Oryza sativa L.) Ecotoxicology and Environmental Safety Heavy metal Oryza sativa Food safety Stable isotope tracer Genetic engineering |
| title | The mechanism of enhanced lignin regulating foliar Cd absorption and yield in rice (Oryza sativa L.) |
| title_full | The mechanism of enhanced lignin regulating foliar Cd absorption and yield in rice (Oryza sativa L.) |
| title_fullStr | The mechanism of enhanced lignin regulating foliar Cd absorption and yield in rice (Oryza sativa L.) |
| title_full_unstemmed | The mechanism of enhanced lignin regulating foliar Cd absorption and yield in rice (Oryza sativa L.) |
| title_short | The mechanism of enhanced lignin regulating foliar Cd absorption and yield in rice (Oryza sativa L.) |
| title_sort | mechanism of enhanced lignin regulating foliar cd absorption and yield in rice oryza sativa l |
| topic | Heavy metal Oryza sativa Food safety Stable isotope tracer Genetic engineering |
| url | http://www.sciencedirect.com/science/article/pii/S0147651322013215 |
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