Cytotoxic Effects of Digoxin on Mesenchymal Stem Cells: An in Vitro Study
Introduction: Cardiac glycosides such as digoxin or digitoxin are the natural products that are traditionally used to increase cardiac contractile force in patients with heart failure and cardiac arrhythmias. It has been shown that digoxin can directly inhibit the cell proliferation and lead to cell...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Negah Institute for Scientific Communication
2014-11-01
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| Series: | Anatomical Sciences Journal |
| Subjects: | |
| Online Access: | http://anatomyjournal.ir/article-1-105-en.html |
| Summary: | Introduction: Cardiac glycosides such as digoxin or digitoxin are the natural products that are
traditionally used to increase cardiac contractile force in patients with heart failure and cardiac
arrhythmias. It has been shown that digoxin can directly inhibit the cell proliferation and lead
to cell apoptosis. Present study was conducted to analyze the effect of digoxin in the cohorts of
mesenchymal stem cells(MSCs) -based therapy. Methods: MSCs were cultured and treated with different concentrations (0.1, 0.5, 1, 5, 7, 10, 15,
20, 30 and 40 μM) of digoxin for 6, 12, 24 and 48 hours. MTT assay was performed to study cell
viability and proliferation. Results: After 24 and 48 hours, cell viability was significantly decreased in 20, 30 and 40 μM
concentrations (P<0.05), but significant decline in cell viability was observed only in 40 μM
after 12 hours (P0.05). Conclusion: It is suggested that digoxin may lead to decline in cell survival ability and increase
in cell apoptosis in a dose-time dependent pattern. It is recommended to consider application of
glycosoids in concurrence with stem cell therapy. |
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| ISSN: | 2322-3626 2322-3626 |