Cytotoxic Effects of Digoxin on Mesenchymal Stem Cells: An in Vitro Study
Introduction: Cardiac glycosides such as digoxin or digitoxin are the natural products that are traditionally used to increase cardiac contractile force in patients with heart failure and cardiac arrhythmias. It has been shown that digoxin can directly inhibit the cell proliferation and lead to cell...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Negah Institute for Scientific Communication
2014-11-01
|
| Series: | Anatomical Sciences Journal |
| Subjects: | |
| Online Access: | http://anatomyjournal.ir/article-1-105-en.html |
| _version_ | 1818043295870222336 |
|---|---|
| author | Hooshang Rafighdoust Amaneh Mohammadi Roushandeh Shirin Darabi Motahareh Mortazavi Amir Rafighdoust |
| author_facet | Hooshang Rafighdoust Amaneh Mohammadi Roushandeh Shirin Darabi Motahareh Mortazavi Amir Rafighdoust |
| author_sort | Hooshang Rafighdoust |
| collection | DOAJ |
| description | Introduction: Cardiac glycosides such as digoxin or digitoxin are the natural products that are
traditionally used to increase cardiac contractile force in patients with heart failure and cardiac
arrhythmias. It has been shown that digoxin can directly inhibit the cell proliferation and lead
to cell apoptosis. Present study was conducted to analyze the effect of digoxin in the cohorts of
mesenchymal stem cells(MSCs) -based therapy. Methods: MSCs were cultured and treated with different concentrations (0.1, 0.5, 1, 5, 7, 10, 15,
20, 30 and 40 μM) of digoxin for 6, 12, 24 and 48 hours. MTT assay was performed to study cell
viability and proliferation. Results: After 24 and 48 hours, cell viability was significantly decreased in 20, 30 and 40 μM
concentrations (P<0.05), but significant decline in cell viability was observed only in 40 μM
after 12 hours (P0.05). Conclusion: It is suggested that digoxin may lead to decline in cell survival ability and increase
in cell apoptosis in a dose-time dependent pattern. It is recommended to consider application of
glycosoids in concurrence with stem cell therapy. |
| first_indexed | 2024-12-10T08:59:57Z |
| format | Article |
| id | doaj.art-b2d5280b6eae4b2a867cc8350d942eac |
| institution | Directory Open Access Journal |
| issn | 2322-3626 2322-3626 |
| language | English |
| last_indexed | 2024-12-10T08:59:57Z |
| publishDate | 2014-11-01 |
| publisher | Negah Institute for Scientific Communication |
| record_format | Article |
| series | Anatomical Sciences Journal |
| spelling | doaj.art-b2d5280b6eae4b2a867cc8350d942eac2022-12-22T01:55:19ZengNegah Institute for Scientific CommunicationAnatomical Sciences Journal2322-36262322-36262014-11-01114183188Cytotoxic Effects of Digoxin on Mesenchymal Stem Cells: An in Vitro StudyHooshang Rafighdoust0Amaneh Mohammadi Roushandeh1Shirin Darabi2Motahareh Mortazavi3Amir Rafighdoust4 Organization Organization Department of Anatomical Sciences, School of Medicine, Zahehan University of Medical Sciences, Zahedan, Iran. Organization Organization Introduction: Cardiac glycosides such as digoxin or digitoxin are the natural products that are traditionally used to increase cardiac contractile force in patients with heart failure and cardiac arrhythmias. It has been shown that digoxin can directly inhibit the cell proliferation and lead to cell apoptosis. Present study was conducted to analyze the effect of digoxin in the cohorts of mesenchymal stem cells(MSCs) -based therapy. Methods: MSCs were cultured and treated with different concentrations (0.1, 0.5, 1, 5, 7, 10, 15, 20, 30 and 40 μM) of digoxin for 6, 12, 24 and 48 hours. MTT assay was performed to study cell viability and proliferation. Results: After 24 and 48 hours, cell viability was significantly decreased in 20, 30 and 40 μM concentrations (P<0.05), but significant decline in cell viability was observed only in 40 μM after 12 hours (P0.05). Conclusion: It is suggested that digoxin may lead to decline in cell survival ability and increase in cell apoptosis in a dose-time dependent pattern. It is recommended to consider application of glycosoids in concurrence with stem cell therapy.http://anatomyjournal.ir/article-1-105-en.htmlmesenchymal stem celldigoxinmttcell proliferation |
| spellingShingle | Hooshang Rafighdoust Amaneh Mohammadi Roushandeh Shirin Darabi Motahareh Mortazavi Amir Rafighdoust Cytotoxic Effects of Digoxin on Mesenchymal Stem Cells: An in Vitro Study Anatomical Sciences Journal mesenchymal stem cell digoxin mtt cell proliferation |
| title | Cytotoxic Effects of Digoxin on Mesenchymal Stem Cells: An in Vitro Study |
| title_full | Cytotoxic Effects of Digoxin on Mesenchymal Stem Cells: An in Vitro Study |
| title_fullStr | Cytotoxic Effects of Digoxin on Mesenchymal Stem Cells: An in Vitro Study |
| title_full_unstemmed | Cytotoxic Effects of Digoxin on Mesenchymal Stem Cells: An in Vitro Study |
| title_short | Cytotoxic Effects of Digoxin on Mesenchymal Stem Cells: An in Vitro Study |
| title_sort | cytotoxic effects of digoxin on mesenchymal stem cells an in vitro study |
| topic | mesenchymal stem cell digoxin mtt cell proliferation |
| url | http://anatomyjournal.ir/article-1-105-en.html |
| work_keys_str_mv | AT hooshangrafighdoust cytotoxiceffectsofdigoxinonmesenchymalstemcellsaninvitrostudy AT amanehmohammadiroushandeh cytotoxiceffectsofdigoxinonmesenchymalstemcellsaninvitrostudy AT shirindarabi cytotoxiceffectsofdigoxinonmesenchymalstemcellsaninvitrostudy AT motaharehmortazavi cytotoxiceffectsofdigoxinonmesenchymalstemcellsaninvitrostudy AT amirrafighdoust cytotoxiceffectsofdigoxinonmesenchymalstemcellsaninvitrostudy |