Doxycycline increases neurogenesis and reduces microglia in the adult hippocampus.
Adult hippocampal neurogenesis results in the continuous formation of new neurons and is a process of brain plasticity involved in learning and memory. Although inducible-reversible transgenic mouse models are increasingly being used to investigate adult neurogenesis, transgene control requires the...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2013-07-01
|
| Series: | Frontiers in Neuroscience |
| Subjects: | |
| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fnins.2013.00131/full |
| _version_ | 1818905702530285568 |
|---|---|
| author | Sebastien eSultan Elias eGebara Nicolas eToni |
| author_facet | Sebastien eSultan Elias eGebara Nicolas eToni |
| author_sort | Sebastien eSultan |
| collection | DOAJ |
| description | Adult hippocampal neurogenesis results in the continuous formation of new neurons and is a process of brain plasticity involved in learning and memory. Although inducible-reversible transgenic mouse models are increasingly being used to investigate adult neurogenesis, transgene control requires the administration of an activator, doxycycline, with unknown effects on adult neurogenesis. Here, we tested the effect of doxycycline administration on adult neurogenesis in vivo. We found that 4 weeks of doxycycline treatment at doses commonly used for gene expression control, resulted in increased neurogenesis. Furthermore, the dendrites of new neurons displayed increased spine density. Concomitantly, Iba1-expressing microglia was reduced by doxycycline treatment. These results indicate that doxycycline treatment may interfere with parameters of relevance for the use of inducible transgenic mice in studies of adult neurogenesis or brain inflammation. |
| first_indexed | 2024-12-19T21:27:32Z |
| format | Article |
| id | doaj.art-b2db7ee4970a47059be869c054e73f27 |
| institution | Directory Open Access Journal |
| issn | 1662-453X |
| language | English |
| last_indexed | 2024-12-19T21:27:32Z |
| publishDate | 2013-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Neuroscience |
| spelling | doaj.art-b2db7ee4970a47059be869c054e73f272022-12-21T20:05:05ZengFrontiers Media S.A.Frontiers in Neuroscience1662-453X2013-07-01710.3389/fnins.2013.0013156863Doxycycline increases neurogenesis and reduces microglia in the adult hippocampus.Sebastien eSultan0Elias eGebara1Nicolas eToni2University of LausanneUniversity of LausanneUniversity of LausanneAdult hippocampal neurogenesis results in the continuous formation of new neurons and is a process of brain plasticity involved in learning and memory. Although inducible-reversible transgenic mouse models are increasingly being used to investigate adult neurogenesis, transgene control requires the administration of an activator, doxycycline, with unknown effects on adult neurogenesis. Here, we tested the effect of doxycycline administration on adult neurogenesis in vivo. We found that 4 weeks of doxycycline treatment at doses commonly used for gene expression control, resulted in increased neurogenesis. Furthermore, the dendrites of new neurons displayed increased spine density. Concomitantly, Iba1-expressing microglia was reduced by doxycycline treatment. These results indicate that doxycycline treatment may interfere with parameters of relevance for the use of inducible transgenic mice in studies of adult neurogenesis or brain inflammation.http://journal.frontiersin.org/Journal/10.3389/fnins.2013.00131/fullDentate GyrusDoxycyclineGene Expression RegulationHippocampusTetracyclineadult neurogenesis |
| spellingShingle | Sebastien eSultan Elias eGebara Nicolas eToni Doxycycline increases neurogenesis and reduces microglia in the adult hippocampus. Frontiers in Neuroscience Dentate Gyrus Doxycycline Gene Expression Regulation Hippocampus Tetracycline adult neurogenesis |
| title | Doxycycline increases neurogenesis and reduces microglia in the adult hippocampus. |
| title_full | Doxycycline increases neurogenesis and reduces microglia in the adult hippocampus. |
| title_fullStr | Doxycycline increases neurogenesis and reduces microglia in the adult hippocampus. |
| title_full_unstemmed | Doxycycline increases neurogenesis and reduces microglia in the adult hippocampus. |
| title_short | Doxycycline increases neurogenesis and reduces microglia in the adult hippocampus. |
| title_sort | doxycycline increases neurogenesis and reduces microglia in the adult hippocampus |
| topic | Dentate Gyrus Doxycycline Gene Expression Regulation Hippocampus Tetracycline adult neurogenesis |
| url | http://journal.frontiersin.org/Journal/10.3389/fnins.2013.00131/full |
| work_keys_str_mv | AT sebastienesultan doxycyclineincreasesneurogenesisandreducesmicrogliaintheadulthippocampus AT eliasegebara doxycyclineincreasesneurogenesisandreducesmicrogliaintheadulthippocampus AT nicolasetoni doxycyclineincreasesneurogenesisandreducesmicrogliaintheadulthippocampus |