Transmucosal triglyceride transport rates in proximal and distal rat intestine in vivo
Transmucosal transport rates for triolein in proximal and distal intestine were compared in unanesthetized rats. Emulsified [1-14C] triolein together with bile and pancreatic juice from donor rats was infused for 6 hr into either the duodenum or the midpoint of the small intestine at such a rate tha...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
1975-07-01
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| Series: | Journal of Lipid Research |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520367110 |
| _version_ | 1818942519566663680 |
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| author | A.L. Wu S.B. Clark P.R. Holt |
| author_facet | A.L. Wu S.B. Clark P.R. Holt |
| author_sort | A.L. Wu |
| collection | DOAJ |
| description | Transmucosal transport rates for triolein in proximal and distal intestine were compared in unanesthetized rats. Emulsified [1-14C] triolein together with bile and pancreatic juice from donor rats was infused for 6 hr into either the duodenum or the midpoint of the small intestine at such a rate that absorption was essentially complete in both regions of the intestine. Lymph was collected from the thoracic duct during triolein infusion and for an additional 6-hr period. The decrease in the rate of lymphatic output of labeled fat was found to follow a simple exponential function in all animals. This rate of decrease (decay rate) was used to calculate the half-times of lipid turnover through the intestinal wall and the fractional output rates. Distal intestine transported lipid 40% more slowly than proximal intestine, and the difference was associated with a greater accumulation of triglyceride in the distal intestinal wall. Chylomicron synthesis and/or release is the rate-limiting step for distal lymphatic fat transport in vivo, whereas fat uptake from the lumen is rate limiting for proximal intestine. |
| first_indexed | 2024-12-20T07:12:43Z |
| format | Article |
| id | doaj.art-b2dd49ef331145cca152c384b7216cce |
| institution | Directory Open Access Journal |
| issn | 0022-2275 |
| language | English |
| last_indexed | 2024-12-20T07:12:43Z |
| publishDate | 1975-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Lipid Research |
| spelling | doaj.art-b2dd49ef331145cca152c384b7216cce2022-12-21T19:48:51ZengElsevierJournal of Lipid Research0022-22751975-07-01164251257Transmucosal triglyceride transport rates in proximal and distal rat intestine in vivoA.L. WuS.B. ClarkP.R. HoltTransmucosal transport rates for triolein in proximal and distal intestine were compared in unanesthetized rats. Emulsified [1-14C] triolein together with bile and pancreatic juice from donor rats was infused for 6 hr into either the duodenum or the midpoint of the small intestine at such a rate that absorption was essentially complete in both regions of the intestine. Lymph was collected from the thoracic duct during triolein infusion and for an additional 6-hr period. The decrease in the rate of lymphatic output of labeled fat was found to follow a simple exponential function in all animals. This rate of decrease (decay rate) was used to calculate the half-times of lipid turnover through the intestinal wall and the fractional output rates. Distal intestine transported lipid 40% more slowly than proximal intestine, and the difference was associated with a greater accumulation of triglyceride in the distal intestinal wall. Chylomicron synthesis and/or release is the rate-limiting step for distal lymphatic fat transport in vivo, whereas fat uptake from the lumen is rate limiting for proximal intestine.http://www.sciencedirect.com/science/article/pii/S0022227520367110 |
| spellingShingle | A.L. Wu S.B. Clark P.R. Holt Transmucosal triglyceride transport rates in proximal and distal rat intestine in vivo Journal of Lipid Research |
| title | Transmucosal triglyceride transport rates in proximal and distal rat intestine in vivo |
| title_full | Transmucosal triglyceride transport rates in proximal and distal rat intestine in vivo |
| title_fullStr | Transmucosal triglyceride transport rates in proximal and distal rat intestine in vivo |
| title_full_unstemmed | Transmucosal triglyceride transport rates in proximal and distal rat intestine in vivo |
| title_short | Transmucosal triglyceride transport rates in proximal and distal rat intestine in vivo |
| title_sort | transmucosal triglyceride transport rates in proximal and distal rat intestine in vivo |
| url | http://www.sciencedirect.com/science/article/pii/S0022227520367110 |
| work_keys_str_mv | AT alwu transmucosaltriglyceridetransportratesinproximalanddistalratintestineinvivo AT sbclark transmucosaltriglyceridetransportratesinproximalanddistalratintestineinvivo AT prholt transmucosaltriglyceridetransportratesinproximalanddistalratintestineinvivo |