Pharmacological activities of chemically characterized essential oils from Haplophyllum tuberculatum (Forssk.)
The present work aimed at characterizing the phytochemical composition of Haplophyllum tuberculatum essential oil (HTEO), assessing its antifungal activity against various fungal strains, evaluating its insecticidal and repulsive properties against Callosobruchus maculatus, and determine its antioxi...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-10-01
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| Series: | Frontiers in Chemistry |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fchem.2023.1251449/full |
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| author | Abdelkrim Agour Ibrahim Mssillou Aimad Allali Mohamed Chebaibi Mohamed Chebaibi Youness El Abdali Azeddin El Barnossi Yousef A. Bin Jardan Gezahign Fentahun Wondmie Hiba-Allah Nafidi Mohammed Bourhia Amina Bari Badiaa Lyoussi Elhoussine Derwich |
| author_facet | Abdelkrim Agour Ibrahim Mssillou Aimad Allali Mohamed Chebaibi Mohamed Chebaibi Youness El Abdali Azeddin El Barnossi Yousef A. Bin Jardan Gezahign Fentahun Wondmie Hiba-Allah Nafidi Mohammed Bourhia Amina Bari Badiaa Lyoussi Elhoussine Derwich |
| author_sort | Abdelkrim Agour |
| collection | DOAJ |
| description | The present work aimed at characterizing the phytochemical composition of Haplophyllum tuberculatum essential oil (HTEO), assessing its antifungal activity against various fungal strains, evaluating its insecticidal and repulsive properties against Callosobruchus maculatus, and determine its antioxidant capacity. To this end, Gas chromatography-mass spectrometry analysis detected 34 compounds in HTEO, with β-Caryophyllene being the major constituent (36.94%). HTEO demonstrated predominantly modest antifungal effects, however, it sustains notable activity, particularly against Aspergillus flavus, with an inhibition rate of 76.50% ± 0.60%. Minimum inhibitory concentrations ranged from 20.53 ± 5.08 to 76.26 ± 5.08 mg/mL, effectively inhibiting fungal growth. Furthermore, the antifungal, and antioxidant activities of HTEO were evaluated in silico against the proteins Aspergillus flavus FAD glucose dehydrogenase, and beta-1,4-endoglucanase from Aspergillus niger, NAD(P)H Oxidase. Moreover, HTEO displayed strong insecticidal activity against C. maculatus, with contact and inhalation tests yielding LC50 values of 30.66 and 40.28 μL/100g, respectively, after 24 h of exposure. A dose of 5 μL/100g significantly reduced oviposition (48.85%) and inhibited emergence (45.15%) compared to the control group. Additionally, HTEO exhibited a high total antioxidant capacity of 758.34 mg AAE/g EO, highlighting its antioxidant potential. Insilico results showed that the antifungal activity of HTEO is mostly attributed to γ-Cadinene and p-Cymen-7-ol, while antioxidant is attributed to α-Terpinyl isobutyrate displayed. Overall, HTEO offers a sustainable and environmentally friendly alternative to synthetic products used to manage diseases. |
| first_indexed | 2024-03-11T19:42:46Z |
| format | Article |
| id | doaj.art-b2de0de3343b4718ac12d34bd4b7bfe5 |
| institution | Directory Open Access Journal |
| issn | 2296-2646 |
| language | English |
| last_indexed | 2024-03-11T19:42:46Z |
| publishDate | 2023-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Chemistry |
| spelling | doaj.art-b2de0de3343b4718ac12d34bd4b7bfe52023-10-06T06:59:29ZengFrontiers Media S.A.Frontiers in Chemistry2296-26462023-10-011110.3389/fchem.2023.12514491251449Pharmacological activities of chemically characterized essential oils from Haplophyllum tuberculatum (Forssk.)Abdelkrim Agour0Ibrahim Mssillou1Aimad Allali2Mohamed Chebaibi3Mohamed Chebaibi4Youness El Abdali5Azeddin El Barnossi6Yousef A. Bin Jardan7Gezahign Fentahun Wondmie8Hiba-Allah Nafidi9Mohammed Bourhia10Amina Bari11Badiaa Lyoussi12Elhoussine Derwich13Laboratory of Natural Substances, Pharmacology, Environment, Modeling, Health, and Quality of Life, Faculty of Sciences Dhar El Mahraz, University Sidi Mohamed Ben Abdellah, Fez, MoroccoLaboratory of Natural Substances, Pharmacology, Environment, Modeling, Health, and Quality of Life, Faculty of Sciences Dhar El Mahraz, University Sidi Mohamed Ben Abdellah, Fez, MoroccoMinistry of Health and Social Protection, Higher Institute of Nursing Professions and Health Techniques, Taza, MoroccoMinistry of Health and Social Protection, Higher Institute of Nursing Professions and Health Techniques, Fez, MoroccoBiomedical and Translational Research Laboratory, Faculty of Medicine and Pharmacy of the Fez, University of Sidi Mohamed Ben Abdellah, Fez, MoroccoLaboratory of Biotechnology, Environment, Agrifood, and Health, Faculty of Sciences Dhar El Mahraz, University of Sidi Mohamed Ben Abdellah, Fez, MoroccoLaboratory of Biotechnology, Environment, Agrifood, and Health, Faculty of Sciences Dhar El Mahraz, University of Sidi Mohamed Ben Abdellah, Fez, MoroccoDepartment of Pharmaceutics, College of Pharmacy, King Saud University, Riyadh, Saudi ArabiaDepartment of Biology, College of Science, Bahir Dar University, Bahir Dar, EthiopiaDepartment of Food Science, Faculty of Agricultural and Food Science, Laval University, Quebec City, QC, CanadaDepartment of Chemistry and Biochemistry, Faculty of Medicine and Pharmacy, Ibn Zohr University, Laayoune, MoroccoLaboratory of Biotechnology, Environment, Agrifood, and Health, Faculty of Sciences Dhar El Mahraz, University of Sidi Mohamed Ben Abdellah, Fez, MoroccoLaboratory of Natural Substances, Pharmacology, Environment, Modeling, Health, and Quality of Life, Faculty of Sciences Dhar El Mahraz, University Sidi Mohamed Ben Abdellah, Fez, MoroccoLaboratory of Natural Substances, Pharmacology, Environment, Modeling, Health, and Quality of Life, Faculty of Sciences Dhar El Mahraz, University Sidi Mohamed Ben Abdellah, Fez, MoroccoThe present work aimed at characterizing the phytochemical composition of Haplophyllum tuberculatum essential oil (HTEO), assessing its antifungal activity against various fungal strains, evaluating its insecticidal and repulsive properties against Callosobruchus maculatus, and determine its antioxidant capacity. To this end, Gas chromatography-mass spectrometry analysis detected 34 compounds in HTEO, with β-Caryophyllene being the major constituent (36.94%). HTEO demonstrated predominantly modest antifungal effects, however, it sustains notable activity, particularly against Aspergillus flavus, with an inhibition rate of 76.50% ± 0.60%. Minimum inhibitory concentrations ranged from 20.53 ± 5.08 to 76.26 ± 5.08 mg/mL, effectively inhibiting fungal growth. Furthermore, the antifungal, and antioxidant activities of HTEO were evaluated in silico against the proteins Aspergillus flavus FAD glucose dehydrogenase, and beta-1,4-endoglucanase from Aspergillus niger, NAD(P)H Oxidase. Moreover, HTEO displayed strong insecticidal activity against C. maculatus, with contact and inhalation tests yielding LC50 values of 30.66 and 40.28 μL/100g, respectively, after 24 h of exposure. A dose of 5 μL/100g significantly reduced oviposition (48.85%) and inhibited emergence (45.15%) compared to the control group. Additionally, HTEO exhibited a high total antioxidant capacity of 758.34 mg AAE/g EO, highlighting its antioxidant potential. Insilico results showed that the antifungal activity of HTEO is mostly attributed to γ-Cadinene and p-Cymen-7-ol, while antioxidant is attributed to α-Terpinyl isobutyrate displayed. Overall, HTEO offers a sustainable and environmentally friendly alternative to synthetic products used to manage diseases.https://www.frontiersin.org/articles/10.3389/fchem.2023.1251449/fullCallosobruchus maculatusHaplophyllum tuberculatumin-silicomoldstored seeds |
| spellingShingle | Abdelkrim Agour Ibrahim Mssillou Aimad Allali Mohamed Chebaibi Mohamed Chebaibi Youness El Abdali Azeddin El Barnossi Yousef A. Bin Jardan Gezahign Fentahun Wondmie Hiba-Allah Nafidi Mohammed Bourhia Amina Bari Badiaa Lyoussi Elhoussine Derwich Pharmacological activities of chemically characterized essential oils from Haplophyllum tuberculatum (Forssk.) Frontiers in Chemistry Callosobruchus maculatus Haplophyllum tuberculatum in-silico mold stored seeds |
| title | Pharmacological activities of chemically characterized essential oils from Haplophyllum tuberculatum (Forssk.) |
| title_full | Pharmacological activities of chemically characterized essential oils from Haplophyllum tuberculatum (Forssk.) |
| title_fullStr | Pharmacological activities of chemically characterized essential oils from Haplophyllum tuberculatum (Forssk.) |
| title_full_unstemmed | Pharmacological activities of chemically characterized essential oils from Haplophyllum tuberculatum (Forssk.) |
| title_short | Pharmacological activities of chemically characterized essential oils from Haplophyllum tuberculatum (Forssk.) |
| title_sort | pharmacological activities of chemically characterized essential oils from haplophyllum tuberculatum forssk |
| topic | Callosobruchus maculatus Haplophyllum tuberculatum in-silico mold stored seeds |
| url | https://www.frontiersin.org/articles/10.3389/fchem.2023.1251449/full |
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