Biallelic and gene-wide genomic substitution for endogenous intron and retroelement mutagenesis in human cells
Functional annotation of the vast noncoding landscape of the diploid human genome still remains a major challenge of genomic research. Here the authors present a scarless, biallelic, and 100 kb-scale mutagenesis in human cells that uncovers functional significances of endogenous introns and retrotra...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2022-07-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-022-31982-1 |
| _version_ | 1811287395716300800 |
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| author | Tomoyuki Ohno Taichi Akase Shunya Kono Hikaru Kurasawa Takuto Takashima Shinya Kaneko Yasunori Aizawa |
| author_facet | Tomoyuki Ohno Taichi Akase Shunya Kono Hikaru Kurasawa Takuto Takashima Shinya Kaneko Yasunori Aizawa |
| author_sort | Tomoyuki Ohno |
| collection | DOAJ |
| description | Functional annotation of the vast noncoding landscape of the diploid human genome still remains a major challenge of genomic research. Here the authors present a scarless, biallelic, and 100 kb-scale mutagenesis in human cells that uncovers functional significances of endogenous introns and retrotransposons in the chromatin context. |
| first_indexed | 2024-04-13T03:17:50Z |
| format | Article |
| id | doaj.art-b2df2d24b88b42f5be9068f87324eaa8 |
| institution | Directory Open Access Journal |
| issn | 2041-1723 |
| language | English |
| last_indexed | 2024-04-13T03:17:50Z |
| publishDate | 2022-07-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj.art-b2df2d24b88b42f5be9068f87324eaa82022-12-22T03:04:52ZengNature PortfolioNature Communications2041-17232022-07-0113111310.1038/s41467-022-31982-1Biallelic and gene-wide genomic substitution for endogenous intron and retroelement mutagenesis in human cellsTomoyuki Ohno0Taichi Akase1Shunya Kono2Hikaru Kurasawa3Takuto Takashima4Shinya Kaneko5Yasunori Aizawa6School of Life Science and Technology, Tokyo Institute of TechnologySchool of Life Science and Technology, Tokyo Institute of TechnologySchool of Life Science and Technology, Tokyo Institute of TechnologySchool of Life Science and Technology, Tokyo Institute of TechnologySchool of Life Science and Technology, Tokyo Institute of TechnologySchool of Life Science and Technology, Tokyo Institute of TechnologySchool of Life Science and Technology, Tokyo Institute of TechnologyFunctional annotation of the vast noncoding landscape of the diploid human genome still remains a major challenge of genomic research. Here the authors present a scarless, biallelic, and 100 kb-scale mutagenesis in human cells that uncovers functional significances of endogenous introns and retrotransposons in the chromatin context.https://doi.org/10.1038/s41467-022-31982-1 |
| spellingShingle | Tomoyuki Ohno Taichi Akase Shunya Kono Hikaru Kurasawa Takuto Takashima Shinya Kaneko Yasunori Aizawa Biallelic and gene-wide genomic substitution for endogenous intron and retroelement mutagenesis in human cells Nature Communications |
| title | Biallelic and gene-wide genomic substitution for endogenous intron and retroelement mutagenesis in human cells |
| title_full | Biallelic and gene-wide genomic substitution for endogenous intron and retroelement mutagenesis in human cells |
| title_fullStr | Biallelic and gene-wide genomic substitution for endogenous intron and retroelement mutagenesis in human cells |
| title_full_unstemmed | Biallelic and gene-wide genomic substitution for endogenous intron and retroelement mutagenesis in human cells |
| title_short | Biallelic and gene-wide genomic substitution for endogenous intron and retroelement mutagenesis in human cells |
| title_sort | biallelic and gene wide genomic substitution for endogenous intron and retroelement mutagenesis in human cells |
| url | https://doi.org/10.1038/s41467-022-31982-1 |
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