Impact of Peels Extracts from an Italian Ancient Tomato Variety Grown under Drought Stress Conditions on Vascular Related Dysfunction

Background: Tomato by-products contain a great variety of biologically active substances and represent a significant source of natural antioxidant supplements of the human diet. The aim of the work was to compare the antioxidant properties of a by-product from an ancient Tuscan tomato variety, Rosso...

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Main Authors: Maria Michela Cesare, Francesca Felice, Veronica Conti, Luca Cerri, Ylenia Zambito, Marco Romi, Giampiero Cai, Claudio Cantini, Rossella Di Stefano
Format: Article
Language:English
Published: MDPI AG 2021-07-01
Series:Molecules
Subjects:
Online Access:https://www.mdpi.com/1420-3049/26/14/4289
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author Maria Michela Cesare
Francesca Felice
Veronica Conti
Luca Cerri
Ylenia Zambito
Marco Romi
Giampiero Cai
Claudio Cantini
Rossella Di Stefano
author_facet Maria Michela Cesare
Francesca Felice
Veronica Conti
Luca Cerri
Ylenia Zambito
Marco Romi
Giampiero Cai
Claudio Cantini
Rossella Di Stefano
author_sort Maria Michela Cesare
collection DOAJ
description Background: Tomato by-products contain a great variety of biologically active substances and represent a significant source of natural antioxidant supplements of the human diet. The aim of the work was to compare the antioxidant properties of a by-product from an ancient Tuscan tomato variety, Rosso di Pitigliano (RED), obtained by growing plants in normal conditions (-Ctr) or in drought stress conditions (-Ds) for their beneficial effects on vascular related dysfunction. Methods: The antioxidant activity and total polyphenol content (TPC) were measured. The identification of bioactive compounds of tomato peel was performed by HPLC. HUVEC were pre-treated with different TPC of RED-Ctr or RED-Ds, then stressed with H<sub>2</sub>O<sub>2</sub>. Cell viability, ROS production and CAT, SOD and GPx activities were evaluated. Permeation of antioxidant molecules contained in RED across excised rat intestine was also studied. Results: RED-Ds tomato peel extract possessed higher TPC than compared to RED-Ctr (361.32 ± 7.204 mg vs. 152.46 ± 1.568 mg GAE/100 g fresh weight). All extracts were non-cytotoxic. Two hour pre-treatment with 5 µg GAE/mL from RED-Ctr or RED-Ds showed protection from H<sub>2</sub>O<sub>2</sub>-induced oxidative stress and significantly reduced ROS production raising SOD and CAT activity (* <i>p</i> < 0.05 and ** <i>p</i> < 0.005 vs. H<sub>2</sub>O<sub>2</sub>, respectively). The permeation of antioxidant molecules contained in RED-Ctr or RED-Ds across excised rat intestine was high with non-significant difference between the two RED types (41.9 ± 9.6% vs. 26.6 ± 7.8%). Conclusions: RED-Ds tomato peel extract represents a good source of bioactive molecules, which protects HUVECs from oxidative stress at low concentration.
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spelling doaj.art-b2e13e72277548f6b43355718fc5e3dc2023-11-22T04:31:30ZengMDPI AGMolecules1420-30492021-07-012614428910.3390/molecules26144289Impact of Peels Extracts from an Italian Ancient Tomato Variety Grown under Drought Stress Conditions on Vascular Related DysfunctionMaria Michela Cesare0Francesca Felice1Veronica Conti2Luca Cerri3Ylenia Zambito4Marco Romi5Giampiero Cai6Claudio Cantini7Rossella Di Stefano8Cardiovascular Research Laboratory, Department of Surgical, Medical and Molecular Pathology and Critical Care Medicine, University of Pisa, 56100 Pisa, ItalyCardiovascular Research Laboratory, Department of Surgical, Medical and Molecular Pathology and Critical Care Medicine, University of Pisa, 56100 Pisa, ItalyDepartment of Life Sciences, University of Siena, Via P.A. Mattioli 4, 53100 Siena, ItalyDepartment of Life Sciences, University of Siena, Via P.A. Mattioli 4, 53100 Siena, ItalyDepartment of Pharmacy, University of Pisa, 56126 Pisa, ItalyDepartment of Life Sciences, University of Siena, Via P.A. Mattioli 4, 53100 Siena, ItalyDepartment of Life Sciences, University of Siena, Via P.A. Mattioli 4, 53100 Siena, ItalyCNR-IBE (Consiglio Nazionale delle Ricerche-Istituto per la Bioeconomia), 58022 Follonica, ItalyCardiovascular Research Laboratory, Department of Surgical, Medical and Molecular Pathology and Critical Care Medicine, University of Pisa, 56100 Pisa, ItalyBackground: Tomato by-products contain a great variety of biologically active substances and represent a significant source of natural antioxidant supplements of the human diet. The aim of the work was to compare the antioxidant properties of a by-product from an ancient Tuscan tomato variety, Rosso di Pitigliano (RED), obtained by growing plants in normal conditions (-Ctr) or in drought stress conditions (-Ds) for their beneficial effects on vascular related dysfunction. Methods: The antioxidant activity and total polyphenol content (TPC) were measured. The identification of bioactive compounds of tomato peel was performed by HPLC. HUVEC were pre-treated with different TPC of RED-Ctr or RED-Ds, then stressed with H<sub>2</sub>O<sub>2</sub>. Cell viability, ROS production and CAT, SOD and GPx activities were evaluated. Permeation of antioxidant molecules contained in RED across excised rat intestine was also studied. Results: RED-Ds tomato peel extract possessed higher TPC than compared to RED-Ctr (361.32 ± 7.204 mg vs. 152.46 ± 1.568 mg GAE/100 g fresh weight). All extracts were non-cytotoxic. Two hour pre-treatment with 5 µg GAE/mL from RED-Ctr or RED-Ds showed protection from H<sub>2</sub>O<sub>2</sub>-induced oxidative stress and significantly reduced ROS production raising SOD and CAT activity (* <i>p</i> < 0.05 and ** <i>p</i> < 0.005 vs. H<sub>2</sub>O<sub>2</sub>, respectively). The permeation of antioxidant molecules contained in RED-Ctr or RED-Ds across excised rat intestine was high with non-significant difference between the two RED types (41.9 ± 9.6% vs. 26.6 ± 7.8%). Conclusions: RED-Ds tomato peel extract represents a good source of bioactive molecules, which protects HUVECs from oxidative stress at low concentration.https://www.mdpi.com/1420-3049/26/14/4289tomato peel extracttomato by-productsoxidative stressdrought stresspolyphenolshuman vascular endothelial cells
spellingShingle Maria Michela Cesare
Francesca Felice
Veronica Conti
Luca Cerri
Ylenia Zambito
Marco Romi
Giampiero Cai
Claudio Cantini
Rossella Di Stefano
Impact of Peels Extracts from an Italian Ancient Tomato Variety Grown under Drought Stress Conditions on Vascular Related Dysfunction
Molecules
tomato peel extract
tomato by-products
oxidative stress
drought stress
polyphenols
human vascular endothelial cells
title Impact of Peels Extracts from an Italian Ancient Tomato Variety Grown under Drought Stress Conditions on Vascular Related Dysfunction
title_full Impact of Peels Extracts from an Italian Ancient Tomato Variety Grown under Drought Stress Conditions on Vascular Related Dysfunction
title_fullStr Impact of Peels Extracts from an Italian Ancient Tomato Variety Grown under Drought Stress Conditions on Vascular Related Dysfunction
title_full_unstemmed Impact of Peels Extracts from an Italian Ancient Tomato Variety Grown under Drought Stress Conditions on Vascular Related Dysfunction
title_short Impact of Peels Extracts from an Italian Ancient Tomato Variety Grown under Drought Stress Conditions on Vascular Related Dysfunction
title_sort impact of peels extracts from an italian ancient tomato variety grown under drought stress conditions on vascular related dysfunction
topic tomato peel extract
tomato by-products
oxidative stress
drought stress
polyphenols
human vascular endothelial cells
url https://www.mdpi.com/1420-3049/26/14/4289
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