Application of RNA-targeted CRISPR/CasRx system in mouse spermatogonia cell line GC1-spg

Application of RNA-targeted CRISPR/CasRx system in mouse spermatogonia cell line GC1-spg

Objective To investigate the application of CRISPR/CasRx mediated gene knockdown at the RNA level in mouse spermatogonia cell line GC1-spg. Methods The EF1a core promoter. CasRx.SV40.U6.DRs expression plasmid (CasRx-gRNA) and three fluorescent protein of EF1a.EGFP, EF1a.mCherry, EF1a.tdToamto expr...

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Bibliographic Details
Main Author:	LI Meng-zhen, LIU Jun, ZOU Ding-feng, MIAO Shi-ying, WANG Lin-fang, SONG Wei, LI Kai
Format:	Article
Language:	zho
Published:	Institute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College. 2021-05-01
Series:	Jichu yixue yu linchuang
Subjects:	crispr/casrx\|rna\|knockdown\|gc1-spg
Online Access:	http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/a210104.pdf

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