Surveillance and analysis of avian influenza viruses in the external environment of poultry in Anqing City， Anhui Province
ObjectiveTo determine the prevalence of avian influenza viruses in the external environment of poultry in Anqing City， Anhui Province， and provide scientific evidence for prevention and control of animal-derived influenza in humans.MethodsA total of 28 farmers’ markets/farms in 10 counties （cities，...
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| Format: | Article |
| Language: | zho |
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Shanghai Preventive Medicine Association
2023-11-01
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| Series: | Shanghai yufang yixue |
| Subjects: | |
| Online Access: | http://www.sjpm.org.cn/cn/article/doi/10.19428/j.cnki.sjpm.2023.23310 |
| _version_ | 1797356764997353472 |
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| author | WU Zhaochun HUANG Xu WANG Jinsheng QIAN Yezhong WANG Jin CHEN Jiwen LI Yuxin |
| author_facet | WU Zhaochun HUANG Xu WANG Jinsheng QIAN Yezhong WANG Jin CHEN Jiwen LI Yuxin |
| author_sort | WU Zhaochun |
| collection | DOAJ |
| description | ObjectiveTo determine the prevalence of avian influenza viruses in the external environment of poultry in Anqing City， Anhui Province， and provide scientific evidence for prevention and control of animal-derived influenza in humans.MethodsA total of 28 farmers’ markets/farms in 10 counties （cities， districts） of Anqing City， Anhui Province， were selected as surveillance sites by simple random sampling strategy. Poultry faeces and other related samples were collected for 6 consecutive weeks. Real-time fluorescence quantitative PCR was used to examine the nucleic acids of influenza A virus. Subtypes H5， H7， and H9 of avian influenza virus were further tested in the positive samples.ResultsA total of 426 specimens were collected， among which 113 tested positive with a positive rate of 26.53%. Among the positive specimens， 104 were determined to be subtype H9， accounting for 92.04%. It did not significantly differ in the positive rate between the main and non-main urban areas （χ2<0.01， P>0.05） or among the specimens collected in different weeks （χ2=7.57， P>0.05）. However， it significantly differed in the positive rate among the specimens collected in the third week and other weeks （χ2=6.89， P<0.05）. Furthermore， among the different sampling sites， farms had the highest positive rate of 46.67%. Among the specimens from different sources， the surface-coated specimens from poultry cages had the highest positive rate of 34.78%.ConclusionAvian influenza viruses are prevalent in the external environment of poultry in Anqing City. It warrants strengthening the surveillance and risk assessment to reduce the virus transmission in the external environment and risk of human infection with animal-derived influenza. |
| first_indexed | 2024-03-08T14:32:21Z |
| format | Article |
| id | doaj.art-b2e6339f4bb545b5855f919c738fafaf |
| institution | Directory Open Access Journal |
| issn | 1004-9231 |
| language | zho |
| last_indexed | 2024-03-08T14:32:21Z |
| publishDate | 2023-11-01 |
| publisher | Shanghai Preventive Medicine Association |
| record_format | Article |
| series | Shanghai yufang yixue |
| spelling | doaj.art-b2e6339f4bb545b5855f919c738fafaf2024-01-12T09:31:20ZzhoShanghai Preventive Medicine AssociationShanghai yufang yixue1004-92312023-11-0135111058106210.19428/j.cnki.sjpm.2023.233101004-9231（2023）11-1058-05Surveillance and analysis of avian influenza viruses in the external environment of poultry in Anqing City， Anhui ProvinceWU Zhaochun0HUANG Xu1WANG Jinsheng2QIAN Yezhong3WANG Jin4CHEN Jiwen5LI Yuxin6Anqing Center for Disease Control and Prevention, Anqing, Anhui 246005, ChinaAnqing Center for Disease Control and Prevention, Anqing, Anhui 246005, ChinaAnqing Center for Disease Control and Prevention, Anqing, Anhui 246005, ChinaTaihu County Center for Disease Control and Prevention, Anqing, Anhui 246400, ChinaWangjiang County Center for Disease Control and Prevention, Anqing, Anhui 246200, ChinaAnqing Center for Disease Control and Prevention, Anqing, Anhui 246005, ChinaAnqing Center for Disease Control and Prevention, Anqing, Anhui 246005, ChinaObjectiveTo determine the prevalence of avian influenza viruses in the external environment of poultry in Anqing City， Anhui Province， and provide scientific evidence for prevention and control of animal-derived influenza in humans.MethodsA total of 28 farmers’ markets/farms in 10 counties （cities， districts） of Anqing City， Anhui Province， were selected as surveillance sites by simple random sampling strategy. Poultry faeces and other related samples were collected for 6 consecutive weeks. Real-time fluorescence quantitative PCR was used to examine the nucleic acids of influenza A virus. Subtypes H5， H7， and H9 of avian influenza virus were further tested in the positive samples.ResultsA total of 426 specimens were collected， among which 113 tested positive with a positive rate of 26.53%. Among the positive specimens， 104 were determined to be subtype H9， accounting for 92.04%. It did not significantly differ in the positive rate between the main and non-main urban areas （χ2<0.01， P>0.05） or among the specimens collected in different weeks （χ2=7.57， P>0.05）. However， it significantly differed in the positive rate among the specimens collected in the third week and other weeks （χ2=6.89， P<0.05）. Furthermore， among the different sampling sites， farms had the highest positive rate of 46.67%. Among the specimens from different sources， the surface-coated specimens from poultry cages had the highest positive rate of 34.78%.ConclusionAvian influenza viruses are prevalent in the external environment of poultry in Anqing City. It warrants strengthening the surveillance and risk assessment to reduce the virus transmission in the external environment and risk of human infection with animal-derived influenza.http://www.sjpm.org.cn/cn/article/doi/10.19428/j.cnki.sjpm.2023.23310external environmentavian influenza virussurveillance |
| spellingShingle | WU Zhaochun HUANG Xu WANG Jinsheng QIAN Yezhong WANG Jin CHEN Jiwen LI Yuxin Surveillance and analysis of avian influenza viruses in the external environment of poultry in Anqing City， Anhui Province Shanghai yufang yixue external environment avian influenza virus surveillance |
| title | Surveillance and analysis of avian influenza viruses in the external environment of poultry in Anqing City， Anhui Province |
| title_full | Surveillance and analysis of avian influenza viruses in the external environment of poultry in Anqing City， Anhui Province |
| title_fullStr | Surveillance and analysis of avian influenza viruses in the external environment of poultry in Anqing City， Anhui Province |
| title_full_unstemmed | Surveillance and analysis of avian influenza viruses in the external environment of poultry in Anqing City， Anhui Province |
| title_short | Surveillance and analysis of avian influenza viruses in the external environment of poultry in Anqing City， Anhui Province |
| title_sort | surveillance and analysis of avian influenza viruses in the external environment of poultry in anqing city anhui province |
| topic | external environment avian influenza virus surveillance |
| url | http://www.sjpm.org.cn/cn/article/doi/10.19428/j.cnki.sjpm.2023.23310 |
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