Effect of Inoculum to Substrate Ratio on the Methane Potential of Microcrystalline Cellulose Production Wastewater

The methane potential and influence of the inoculum to substrate ratio of wastewater originating from the production of microcrystalline cellulose (MCC) were studied. Laboratory experiments were carried out in a continuously stirred batch multi-reactor at mesophilic temperature (37 °C). Inoculum to...

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Main Authors: Lourdes Maria Rodriguez-Chiang, Olli Pekka Dahl
Format: Article
Language:English
Published: North Carolina State University 2014-12-01
Series:BioResources
Subjects:
Online Access:http://ojs.cnr.ncsu.edu/index.php/BioRes/article/view/BioRes_10_1_898_Rodriguez_Chiang_Inoculum_Methane_Potential_Wastewater
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author Lourdes Maria Rodriguez-Chiang
Olli Pekka Dahl
author_facet Lourdes Maria Rodriguez-Chiang
Olli Pekka Dahl
author_sort Lourdes Maria Rodriguez-Chiang
collection DOAJ
description The methane potential and influence of the inoculum to substrate ratio of wastewater originating from the production of microcrystalline cellulose (MCC) were studied. Laboratory experiments were carried out in a continuously stirred batch multi-reactor at mesophilic temperature (37 °C). Inoculum to substrate ratios (ISRs) of 2.0, 1.0, 0.8, and 0.5 based on volatile solids (VS) were evaluated. The results demonstrate the suitability of MCC wastewater at ISRs of 2.0, 1.0, and 0.8 with ultimate methane potentials of 333, 297, and 325 mL CH4 per gram of volatile solids added, respectively, which correspond to anaerobic degradabilities of 91.4, 81.7, and 89.3%, respectively, compared to the theoretical potential. The inoculum to substrate ratio of 2.0 provided a faster methane production rate and a kinetic constant of 0.24 d-1, reaching its ultimate yield at day 8 of incubation. The lowest ISR of 0.5 showed the occurrence of process inhibition due to accumulation of acids. Energy estimation suggests that considering the volume and VS of wastewater produced in a MCC mill, a total energy amount of 44,105 GJ/year can be produced, which can be used to replace 29.4% of the natural gas demand.
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spelling doaj.art-b2e7040906c44380833a1e1044b6974e2022-12-21T18:22:54ZengNorth Carolina State UniversityBioResources1930-21261930-21262014-12-0110189891110.15376/biores.10.1.898-911Effect of Inoculum to Substrate Ratio on the Methane Potential of Microcrystalline Cellulose Production WastewaterLourdes Maria Rodriguez-Chiang0Olli Pekka Dahl1Aalto University; FinlandAalto University; FinlandThe methane potential and influence of the inoculum to substrate ratio of wastewater originating from the production of microcrystalline cellulose (MCC) were studied. Laboratory experiments were carried out in a continuously stirred batch multi-reactor at mesophilic temperature (37 °C). Inoculum to substrate ratios (ISRs) of 2.0, 1.0, 0.8, and 0.5 based on volatile solids (VS) were evaluated. The results demonstrate the suitability of MCC wastewater at ISRs of 2.0, 1.0, and 0.8 with ultimate methane potentials of 333, 297, and 325 mL CH4 per gram of volatile solids added, respectively, which correspond to anaerobic degradabilities of 91.4, 81.7, and 89.3%, respectively, compared to the theoretical potential. The inoculum to substrate ratio of 2.0 provided a faster methane production rate and a kinetic constant of 0.24 d-1, reaching its ultimate yield at day 8 of incubation. The lowest ISR of 0.5 showed the occurrence of process inhibition due to accumulation of acids. Energy estimation suggests that considering the volume and VS of wastewater produced in a MCC mill, a total energy amount of 44,105 GJ/year can be produced, which can be used to replace 29.4% of the natural gas demand.http://ojs.cnr.ncsu.edu/index.php/BioRes/article/view/BioRes_10_1_898_Rodriguez_Chiang_Inoculum_Methane_Potential_WastewaterBiogasAnaerobic digestionWastewaterBiochemical methane potentialInoculum to substrate ratioMicrocrystalline cellulose
spellingShingle Lourdes Maria Rodriguez-Chiang
Olli Pekka Dahl
Effect of Inoculum to Substrate Ratio on the Methane Potential of Microcrystalline Cellulose Production Wastewater
BioResources
Biogas
Anaerobic digestion
Wastewater
Biochemical methane potential
Inoculum to substrate ratio
Microcrystalline cellulose
title Effect of Inoculum to Substrate Ratio on the Methane Potential of Microcrystalline Cellulose Production Wastewater
title_full Effect of Inoculum to Substrate Ratio on the Methane Potential of Microcrystalline Cellulose Production Wastewater
title_fullStr Effect of Inoculum to Substrate Ratio on the Methane Potential of Microcrystalline Cellulose Production Wastewater
title_full_unstemmed Effect of Inoculum to Substrate Ratio on the Methane Potential of Microcrystalline Cellulose Production Wastewater
title_short Effect of Inoculum to Substrate Ratio on the Methane Potential of Microcrystalline Cellulose Production Wastewater
title_sort effect of inoculum to substrate ratio on the methane potential of microcrystalline cellulose production wastewater
topic Biogas
Anaerobic digestion
Wastewater
Biochemical methane potential
Inoculum to substrate ratio
Microcrystalline cellulose
url http://ojs.cnr.ncsu.edu/index.php/BioRes/article/view/BioRes_10_1_898_Rodriguez_Chiang_Inoculum_Methane_Potential_Wastewater
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AT ollipekkadahl effectofinoculumtosubstrateratioonthemethanepotentialofmicrocrystallinecelluloseproductionwastewater