Expression of the <i>ripAA</i> Gene in the Soilborne <i>Pseudomonas mosselii</i> Can Promote the Control Efficacy against Tobacco Bacterial Wilt
The environmental bacterium <i>Pseudomonas mosselii</i> produces antagonistic secondary metabolites with inhibitory effects on multiple plant pathogens, including <i>Ralstonia solanacearum,</i> the causal agent of bacterial wilt. In this study, an engineered <i>P. mosse...
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MDPI AG
2022-08-01
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| author | Tao Zhuo Shiting Chen Dandan Wang Xiaojing Fan Xiaofeng Zhang Huasong Zou |
| author_facet | Tao Zhuo Shiting Chen Dandan Wang Xiaojing Fan Xiaofeng Zhang Huasong Zou |
| author_sort | Tao Zhuo |
| collection | DOAJ |
| description | The environmental bacterium <i>Pseudomonas mosselii</i> produces antagonistic secondary metabolites with inhibitory effects on multiple plant pathogens, including <i>Ralstonia solanacearum,</i> the causal agent of bacterial wilt. In this study, an engineered <i>P. mosselii</i> strain was generated to express <i>R. solanacearum ripAA</i>, which determines the incompatible interactions with tobacco plants. The <i>ripAA</i> gene, together with its native promoter, was integrated into the <i>P. mosselii</i> chromosome. The resulting strain showed no difference in antimicrobial activity against <i>R. solanacearum</i>. Promoter-LacZ fusion and RT-PCR experiments demonstrated that the <i>ripAA</i> gene was transcribed in culture media. Compared with that of the wild type, the engineered strain reduced the disease index by 9.1% for bacterial wilt on tobacco plants. A transcriptome analysis was performed to identify differentially expressed genes in tobacco plants, and the results revealed that ethylene- and jasmonate-dependent defense signaling pathways were induced. These data demonstrates that the engineered <i>P. mosselii</i> expressing <i>ripAA</i> can improve biological control against tobacco bacterial wilt by the activation of host defense responses. |
| first_indexed | 2024-03-09T10:00:41Z |
| format | Article |
| id | doaj.art-b2eeefb9cc4d4ecea72a12b4484ac2de |
| institution | Directory Open Access Journal |
| issn | 2079-7737 |
| language | English |
| last_indexed | 2024-03-09T10:00:41Z |
| publishDate | 2022-08-01 |
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| spelling | doaj.art-b2eeefb9cc4d4ecea72a12b4484ac2de2023-12-01T23:26:14ZengMDPI AGBiology2079-77372022-08-01118117010.3390/biology11081170Expression of the <i>ripAA</i> Gene in the Soilborne <i>Pseudomonas mosselii</i> Can Promote the Control Efficacy against Tobacco Bacterial WiltTao Zhuo0Shiting Chen1Dandan Wang2Xiaojing Fan3Xiaofeng Zhang4Huasong Zou5State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaState Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaState Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaState Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaState Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaState Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaThe environmental bacterium <i>Pseudomonas mosselii</i> produces antagonistic secondary metabolites with inhibitory effects on multiple plant pathogens, including <i>Ralstonia solanacearum,</i> the causal agent of bacterial wilt. In this study, an engineered <i>P. mosselii</i> strain was generated to express <i>R. solanacearum ripAA</i>, which determines the incompatible interactions with tobacco plants. The <i>ripAA</i> gene, together with its native promoter, was integrated into the <i>P. mosselii</i> chromosome. The resulting strain showed no difference in antimicrobial activity against <i>R. solanacearum</i>. Promoter-LacZ fusion and RT-PCR experiments demonstrated that the <i>ripAA</i> gene was transcribed in culture media. Compared with that of the wild type, the engineered strain reduced the disease index by 9.1% for bacterial wilt on tobacco plants. A transcriptome analysis was performed to identify differentially expressed genes in tobacco plants, and the results revealed that ethylene- and jasmonate-dependent defense signaling pathways were induced. These data demonstrates that the engineered <i>P. mosselii</i> expressing <i>ripAA</i> can improve biological control against tobacco bacterial wilt by the activation of host defense responses.https://www.mdpi.com/2079-7737/11/8/1170<i>Pseudomonas mosselii</i><i>ripAA</i>bacterial wiltdefense signaling pathwaycontrol efficacy |
| spellingShingle | Tao Zhuo Shiting Chen Dandan Wang Xiaojing Fan Xiaofeng Zhang Huasong Zou Expression of the <i>ripAA</i> Gene in the Soilborne <i>Pseudomonas mosselii</i> Can Promote the Control Efficacy against Tobacco Bacterial Wilt Biology <i>Pseudomonas mosselii</i> <i>ripAA</i> bacterial wilt defense signaling pathway control efficacy |
| title | Expression of the <i>ripAA</i> Gene in the Soilborne <i>Pseudomonas mosselii</i> Can Promote the Control Efficacy against Tobacco Bacterial Wilt |
| title_full | Expression of the <i>ripAA</i> Gene in the Soilborne <i>Pseudomonas mosselii</i> Can Promote the Control Efficacy against Tobacco Bacterial Wilt |
| title_fullStr | Expression of the <i>ripAA</i> Gene in the Soilborne <i>Pseudomonas mosselii</i> Can Promote the Control Efficacy against Tobacco Bacterial Wilt |
| title_full_unstemmed | Expression of the <i>ripAA</i> Gene in the Soilborne <i>Pseudomonas mosselii</i> Can Promote the Control Efficacy against Tobacco Bacterial Wilt |
| title_short | Expression of the <i>ripAA</i> Gene in the Soilborne <i>Pseudomonas mosselii</i> Can Promote the Control Efficacy against Tobacco Bacterial Wilt |
| title_sort | expression of the i ripaa i gene in the soilborne i pseudomonas mosselii i can promote the control efficacy against tobacco bacterial wilt |
| topic | <i>Pseudomonas mosselii</i> <i>ripAA</i> bacterial wilt defense signaling pathway control efficacy |
| url | https://www.mdpi.com/2079-7737/11/8/1170 |
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