Functional Characterization of the Photosynthetic Machinery in <i>Smicronix</i> Galls on the Parasitic Plant <i>Cuscuta campestris</i> by JIP-Test
Members of the genus <i>Cuscuta</i> are generally considered to be non-photosynthetic, stem-holoparasitic flowering plants. Under certain circumstances, at least some members of the genus are capable of limited photosynthesis. The galls of the <i>Smicronyx</i> weevils formed...
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2021-06-01
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| author | Lyuben Zagorchev Alexandra Atanasova Ivanela Albanova Anelia Traianova Petko Mladenov Margarita Kouzmanova Vasilij Goltsev Hazem M. Kalaji Denitsa Teofanova |
| author_facet | Lyuben Zagorchev Alexandra Atanasova Ivanela Albanova Anelia Traianova Petko Mladenov Margarita Kouzmanova Vasilij Goltsev Hazem M. Kalaji Denitsa Teofanova |
| author_sort | Lyuben Zagorchev |
| collection | DOAJ |
| description | Members of the genus <i>Cuscuta</i> are generally considered to be non-photosynthetic, stem-holoparasitic flowering plants. Under certain circumstances, at least some members of the genus are capable of limited photosynthesis. The galls of the <i>Smicronyx</i> weevils formed on <i>Cuscuta campestris</i> are particularly rich in chlorophylls compared to the stem of the parasitic plant. In the present study, we aimed to characterize the photosynthetic activity in the inner and outer gall cortices in comparison to the non-photosynthetic stems and a reference plant (<i>Arabidopsis thaliana</i>). The recorded prompt chlorophyll fluorescence transients were analyzed using JIP test. Detailed analysis of the chlorophyll fluorescence confirmed the presence of actively functioning photosynthetic machinery, especially in the inner cortex of the galls. This photosynthesis, induced by the insect larvae, did not reach the levels of the photosynthetic activity in <i>Arabidopsis thaliana</i> plants. Thylakoid protein complexes were identified by separation with two-dimensional Blue Native/SDS PAGE. It appeared that some of the complexes presented in <i>A. thaliana</i> are missing in <i>C. campestris.</i> We hypothesize that the insect-triggered transition from non-photosynthetic to photosynthetic tissue in the gall is driven by the increased requirements for nutrients related to the larval nutrition. |
| first_indexed | 2024-03-10T10:41:16Z |
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| issn | 2073-4409 |
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| spelling | doaj.art-b2f0e60811ce4842949cca13aa4615a12023-11-21T22:56:32ZengMDPI AGCells2073-44092021-06-01106139910.3390/cells10061399Functional Characterization of the Photosynthetic Machinery in <i>Smicronix</i> Galls on the Parasitic Plant <i>Cuscuta campestris</i> by JIP-TestLyuben Zagorchev0Alexandra Atanasova1Ivanela Albanova2Anelia Traianova3Petko Mladenov4Margarita Kouzmanova5Vasilij Goltsev6Hazem M. Kalaji7Denitsa Teofanova8Faculty of Biology, Sofia University “St. Kliment Ohridski”, 8 Dragan Tsankov Blvd., 1164 Sofia, BulgariaFaculty of Biology, Sofia University “St. Kliment Ohridski”, 8 Dragan Tsankov Blvd., 1164 Sofia, BulgariaFaculty of Biology, Sofia University “St. Kliment Ohridski”, 8 Dragan Tsankov Blvd., 1164 Sofia, BulgariaFaculty of Biology, Sofia University “St. Kliment Ohridski”, 8 Dragan Tsankov Blvd., 1164 Sofia, BulgariaAgroBioInstitute, Agricultural Academy, 8 Dragan Tsankov Blvd., 1164 Sofia, BulgariaFaculty of Biology, Sofia University “St. Kliment Ohridski”, 8 Dragan Tsankov Blvd., 1164 Sofia, BulgariaFaculty of Biology, Sofia University “St. Kliment Ohridski”, 8 Dragan Tsankov Blvd., 1164 Sofia, BulgariaInstitute of Biology, Warsaw University of Life Sciences–SGGW, 02–787 Warsaw, PolandFaculty of Biology, Sofia University “St. Kliment Ohridski”, 8 Dragan Tsankov Blvd., 1164 Sofia, BulgariaMembers of the genus <i>Cuscuta</i> are generally considered to be non-photosynthetic, stem-holoparasitic flowering plants. Under certain circumstances, at least some members of the genus are capable of limited photosynthesis. The galls of the <i>Smicronyx</i> weevils formed on <i>Cuscuta campestris</i> are particularly rich in chlorophylls compared to the stem of the parasitic plant. In the present study, we aimed to characterize the photosynthetic activity in the inner and outer gall cortices in comparison to the non-photosynthetic stems and a reference plant (<i>Arabidopsis thaliana</i>). The recorded prompt chlorophyll fluorescence transients were analyzed using JIP test. Detailed analysis of the chlorophyll fluorescence confirmed the presence of actively functioning photosynthetic machinery, especially in the inner cortex of the galls. This photosynthesis, induced by the insect larvae, did not reach the levels of the photosynthetic activity in <i>Arabidopsis thaliana</i> plants. Thylakoid protein complexes were identified by separation with two-dimensional Blue Native/SDS PAGE. It appeared that some of the complexes presented in <i>A. thaliana</i> are missing in <i>C. campestris.</i> We hypothesize that the insect-triggered transition from non-photosynthetic to photosynthetic tissue in the gall is driven by the increased requirements for nutrients related to the larval nutrition.https://www.mdpi.com/2073-4409/10/6/1399chlorophyll fluorescenceinsect gallsJIP-testparasitic plants |
| spellingShingle | Lyuben Zagorchev Alexandra Atanasova Ivanela Albanova Anelia Traianova Petko Mladenov Margarita Kouzmanova Vasilij Goltsev Hazem M. Kalaji Denitsa Teofanova Functional Characterization of the Photosynthetic Machinery in <i>Smicronix</i> Galls on the Parasitic Plant <i>Cuscuta campestris</i> by JIP-Test Cells chlorophyll fluorescence insect galls JIP-test parasitic plants |
| title | Functional Characterization of the Photosynthetic Machinery in <i>Smicronix</i> Galls on the Parasitic Plant <i>Cuscuta campestris</i> by JIP-Test |
| title_full | Functional Characterization of the Photosynthetic Machinery in <i>Smicronix</i> Galls on the Parasitic Plant <i>Cuscuta campestris</i> by JIP-Test |
| title_fullStr | Functional Characterization of the Photosynthetic Machinery in <i>Smicronix</i> Galls on the Parasitic Plant <i>Cuscuta campestris</i> by JIP-Test |
| title_full_unstemmed | Functional Characterization of the Photosynthetic Machinery in <i>Smicronix</i> Galls on the Parasitic Plant <i>Cuscuta campestris</i> by JIP-Test |
| title_short | Functional Characterization of the Photosynthetic Machinery in <i>Smicronix</i> Galls on the Parasitic Plant <i>Cuscuta campestris</i> by JIP-Test |
| title_sort | functional characterization of the photosynthetic machinery in i smicronix i galls on the parasitic plant i cuscuta campestris i by jip test |
| topic | chlorophyll fluorescence insect galls JIP-test parasitic plants |
| url | https://www.mdpi.com/2073-4409/10/6/1399 |
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